Unlock instant, AI-driven research and patent intelligence for your innovation.

Time-resolved fluorescence immunoassay kit for detecting malachite green

A time-resolved fluorescence and malachite green technology, applied in biological testing, fluorescence/phosphorescence, analytical materials, etc., can solve the problems of complex sample pretreatment, high detection cost, and expensive instruments, and achieve simple operation, wide application, and specificity strong effect

Inactive Publication Date: 2020-07-03
ZHENJIANG XIANCHUANG BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the accuracy of these methods is high, the instruments are expensive, the sample pretreatment is complicated, time-consuming, and the detection cost is high.
At present, there are no patents and literature reports on the time-based fluorescent immunoassay for the detection of malachite green malachite green

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1. Preparation of immunogen and coating agent

[0023] Synthesis of the immunogen (malachite green-BSA) of the present invention: Accurately weigh 324mg of malachite green and dissolve in 2mL N,N-dimethylformamide, add γ-aminobutyric acid solution drop by drop under stirring, and stir for 3 Hours, adjust the pH of the reaction solution to about 10. The precipitate was removed by centrifugation. Add the above reaction dropwise to the BSA solution (320mg BSA dissolved in 5mL physiological saline), then add 23mg of N-hydroxysuccinimide (NHS), 45.4mg of N,N-dicyclohexylcarbodiimide (DCC), React overnight at 4°C, remove the precipitate by centrifugation, take the supernatant and dialyze it with phosphate buffered solution (PBS) for 3 days, change the dialysate every 6 hours, freeze-dry the obtained product, and store it at -20°C for later use;

[0024] Synthesis of coating source (malachite green-OVA): In the above reaction, after replacing BSA with OVA, the reaction conju...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a time-resolved fluorescence immunoassay kit for detecting malachite green. The time-resolved fluorescence immunoassay detection kit for detecting malachite green is composed of a porous coating plate, a buffer solution, a malachite green standard substance, a malachite green antibody freeze-dried product, a europium-labeled goat anti-mouse antibody, a washing solution andan enhancing solution. A detection method of the time-resolved fluorescence immunoassay kit for detecting malachite green comprises the following steps: (1) preparing immunogen; (2) preparing a coating antigen; (3) preparing a monoclonal antibody; and (4) pretreating and detecting a sample. The kit and the method are short in detection time, high in average recovery rate, simple in sample pretreatment, wide in application and low in detection cost, can be operated and detected on site, and have the advantages of high detection specificity, small intra-batch and inter-batch difference, high sensitivity, simplicity and quickness in operation, particular suitability for detection of large-batch samples and the like.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a time-resolved fluorescent immunoassay kit for detecting malachite green malachite green. Background technique [0002] Malachite Green (MG) is a triphenylmethane industrial dye, commonly used in ceramics, textiles, leather, food colorants and cell chemical staining agents. It can also be used as an insect repellant, insecticide, and preservative. It is a class with strong antibacterial effect among medicinal dyes, and is used to prevent and treat saprolegniasis, gill mold and melon worm disease of various aquatic animals. Wait. In recent years, it has been found that malachite green (MG), especially its metabolite colorless malachite green (LMG), has obvious accumulation residues in aquatic animals, and its chemical functional group, triphenylmethane, is a kind of carcinogen. The immune system and reproductive system are greatly affected after the aquatic produ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64G01N33/543G01N33/577G01N33/68
CPCG01N21/6408G01N21/643G01N33/543G01N33/577G01N33/68
Inventor 杜霞洪霞秦悦
Owner ZHENJIANG XIANCHUANG BIOTECH CO LTD