Method for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

An ultra-high-efficiency liquid phase and steroid hormone technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited clinical application, complicated operation, high cost, etc., and achieve the effect of good selectivity, high sensitivity, and low cost

Active Publication Date: 2020-07-10
NANJING QLIFE MEDICAL TESTING LAB CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many problems in the detection of steroid hormones by the current LC-MS / MS technology. Solid-phase extraction or derivatization methods are mostly used for pretreatment, which is complicated to operate and high in cost, and its clinical application is relatively limited.

Method used

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  • Method for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Method for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Method for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

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Embodiment 1

[0097] 1. Experimental materials and instruments

[0098] 1. Materials

[0099] The samples came from serum samples collected from the outpatient clinic of Wuhan Asia Heart Hospital in March 2018.

[0100] (1) Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; ultrapure water instrument (ELGALabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glass instruments, graduated cylinders, etc.

[0101] (2) Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); HPLC grade methyl tert-butyl ether (Fisher, USA); chromatographic column Kinetex XB-C18 (3.0×50mm, 2.6 μm) (Phenomenex, USA).

[0102] (3) Standard products: P4, 17α-OHP4, CORT, T, DHT, AD, E1 and F were purchased from Dr. Eh...

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Abstract

The invention relates to a method for detecting 12 steroid hormones in serum by using an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The method comprises the following steps of: mixing a serum sample with a mixed internal standard solution of all to-be-detected substances, and carrying out one-step liquid-liquid extraction to obtain a to-be-detected solution;adding electrolyte ammonium fluoride into a mobile phase, so that the ionization efficiency of certain target compounds can be effectively improved; and simultaneously detecting the 12 hormones by adopting a mass spectrum positive-negative switching scanning mode. The method has the advantages of multiple detection types, high sensitivity, high specificity, low cost and simple pretreatment process, does not need solid phase extraction or derivation, can complete separation and detection of 12 hormones within 5.0 min, and provides a reliable detection method for the health assessment of clinical endocrine diseases.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a method for detecting 12 steroid hormones in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Steroid hormones are a class of fat-soluble small molecule hormones, also known as steroid hormones. They are produced by cholesterol through a series of enzyme-catalyzed metabolism, and play an important role in regulating the body's metabolism, immune regulation, sexual function regulation, growth and development, etc. Steroid hormones can be divided into progestins, mineralocorticoids, glucocorticoids, estrogens, and androgens according to their physiological functions. Progesterone, corticosterone, and aldosterone are mineralocorticoids; 17α-hydroxyprogesterone and cortisol are glucocorticoids; estrone and estradiol are estrogens; dehydroepiandrosterone sulfate, dehydroepiandrosterone, testosterone, B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/50G01N30/72
CPCG01N30/02G01N30/50G01N30/72G01N2030/045
Inventor 成晓亮李美娟
Owner NANJING QLIFE MEDICAL TESTING LAB CO LTD
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