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Preparation method and application of grateloupia filicina polysaccharide-nano-selenium

A centipede algae polysaccharide and nano-selenium technology, which is applied in the application, skin care preparations, and polysaccharide/gum-containing food ingredients, etc. The effect of high bioactivity and bioavailability

Pending Publication Date: 2020-07-14
GUANGDONG PHARMA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At the same time, oxidative stress often causes elevated levels of free radicals in the body, which can have deleterious effects on proteins, lipids, and DNA

Method used

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  • Preparation method and application of grateloupia filicina polysaccharide-nano-selenium
  • Preparation method and application of grateloupia filicina polysaccharide-nano-selenium
  • Preparation method and application of grateloupia filicina polysaccharide-nano-selenium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The synthesis of embodiment 1 centipede algae polysaccharide and the mensuration of extraction rate

[0051] 1. Preparation of Glucose Standard Curve

[0052] 1.1 Preparation of glucose standards with different concentrations

[0053] Accurately weigh 0.025g glucose standard. After adding an appropriate amount of deionized water to dissolve, transfer to a 50mL volumetric flask and dilute to the mark to obtain a 0.500mg / mL glucose reference solution. Precisely draw 2.00, 3.00, 4.00, 5.00, 6.00mL of 0.500mg / mL glucose standard solution into a 25mL volumetric flask to obtain 0.04, 0.06, 0.08, 0.10, 0.12mg / mL glucose solution.

[0054] 1.2 Phenol-sulfuric acid colorimetric method

[0055] Draw 1.0 mL of the above-mentioned solutions of different concentrations into a test tube, slowly add 1.0 mL of 5% phenol solution in an ice-water bath, and shake well. Quickly add 5 mL of concentrated sulfuric acid solution to the test tube, shake for 5 min, and place in a boiling wat...

Embodiment 2

[0098] Example 2 Preparation and Characterization of Centipede Algae Polysaccharide-Nano Selenium

[0099] 1. Solution preparation

[0100] 1.1 0.1M sodium selenite solution

[0101] Accurately weigh 1.729 g of sodium selenite, use deionized water as solvent, dissolve it in a beaker with an appropriate amount of deionized water, transfer it to a 100 mL volumetric flask, and add deionized water to make it up to the mark.

[0102] 1.2 0.1M ascorbic acid solution

[0103] Accurately weigh 0.440 g of ascorbic acid, use deionized water as solvent, dissolve in a beaker with an appropriate amount of deionized water, transfer it to a 25mL volumetric flask, and add deionized water to make it up to the mark.

[0104] 1.3 0.5% polyvinyl alcohol solution

[0105]Precisely weigh 0.500g of polyvinyl alcohol, use deionized water as the solvent, dissolve it in a beaker with an appropriate amount of deionized water, transfer it to a 100mL volumetric flask, and add deionized water to make it...

Embodiment 3

[0118] Embodiment 3 centipede algal polysaccharide, nano-selenium, centipede algal polysaccharide-nanometer selenium antioxidant test

[0119] 3.1 DPPH free radical scavenging experiment

[0120] 3.1.1 Preparation of solution

[0121] (1) Accurately weigh 0.0025g of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), dissolve it with a small amount of 70% ethanol, transfer it to a 50ml brown volumetric flask, and dilute to volume with 70% ethanol to obtain 0.05mg / mLDPPH test solution, stored in the refrigerator, ready for immediate use;

[0122] (2) Take the above-mentioned centipede algae crude polysaccharide solution, nano-selenium solution and polysaccharide-nano-selenium solution, and prepare solutions with concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg / mL, respectively.

[0123] 3.1.2 Determination of clearance rate

[0124] Accurately draw 5.00mL of sample solution, add 5.00mL of DPPH solution (0.05mg / mL), shake fully, and stand in the dark for 30min. Measure the absorba...

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Abstract

The invention discloses a preparation method and application of grateloupia filicina polysaccharide-nano-selenium and belongs to the technical field of functionalized nano-selenium. According to the method, a grateloupia filicina polysaccharide solution and a sodium selenite solution are mixed, an ascorbic acid solution is dropwise added to the mixed solution, and ultrasonic reaction is conductedto obtain the nano-selenium. The selenium is prepared to be at a nano level, and the obtained nano-selenium has very high bioactivity and bioavailability. The nano-selenium has lower toxicity than traditional selenium products, and a new idea and new development are provided for developing selenium supplement products. Polysaccharide and the nano-selenium are combined, drug administration advantages of a nano-carrier are reserved, and template activity is also reserved. The polysaccharide-nano-selenium becomes a new method for preparing food or medical nano-selenium.

Description

technical field [0001] The invention relates to the technical field of functionalized nano-selenium, in particular to a preparation method and application of centipede algal polysaccharide-nano-selenium. Background technique [0002] Centipede algae (Grateloupia filicina C.Ag.) belongs to Rhodophyta, Euerythrophyta, Cryptocystaceae, Hymenaceae, and Centipede algae. The algae are mostly red or purple, flaky, soft or slightly hard , growing in clusters, mostly on rocky reefs or in bogs near the tide line. Seaweed has specific requirements for the growth environment, and grows in marine environments such as high pressure, high salinity, low light, and low oxygen. It has been used as a marine traditional Chinese medicine since ancient times. It has the effects of clearing heat, detoxifying and deworming, and is rich in resources. It is widely distributed in the coastal areas of the country and is eaten by local people. As the main chemical component of centipede algae, polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A23L33/16C08B37/00A23L5/30A61K33/04A61K47/36A61P39/06A61K8/23A61Q19/00A61Q19/08A61K31/715
CPCA23L33/125A23L33/16C08B37/0036C08B37/0003A23L5/32A61K33/04A61K31/715A61K47/36A61P39/06A61K8/23A61Q19/00A61Q19/08A61K2800/522A61K2800/10A23V2002/00A61K2300/00A23V2250/51A23V2250/1626A23V2300/14A23V2300/48
Inventor 贲永光范晓丹曹碧琅杨月玲
Owner GUANGDONG PHARMA UNIV
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