Method for promoting alcohol production of biomass material

A biomass material and enzymatic hydrolysis technology, applied in the field of promoting biomass material alcohol production, can solve the problems that fermentation inhibitors cannot be effectively reduced, the enzymatic hydrolysis efficiency is not significantly improved, and the cost is high, and the sugar alcohol conversion rate can be improved, and the cost is low. , Strong anti-stress effect

Active Publication Date: 2020-07-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dosing additives (such as surfactants, etc.) in the enzymatic hydrolysis process is one of the effective ways to improve enzymatic hydrolysis efficiency and sugar alcohol conversion efficiency. Some progress has been made in this area, but the following problems still exist in the selection of additives to add: 1. The efficiency of enzymatic hydrolysis is not significantly improved; 2. The fermentation inhibitors cannot be effectively reduced; 3. The cost is high

Method used

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  • Method for promoting alcohol production of biomass material
  • Method for promoting alcohol production of biomass material
  • Method for promoting alcohol production of biomass material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Extraction of grain amaranth protein

[0051] Take the fresh leaf tissue of amaranth in the growth period, grind it with liquid nitrogen, add 0.2M NaAC (pH=4.8) as a buffer, grind until homogenized, centrifuge at 40000rpm at 4°C for 10min, measure the protein concentration in the supernatant by Brandford method, and measure the protein concentration in the supernatant. When the protein concentration in the medium is more than 10ug / ml, store it at -20°C until use.

[0052] (2) Pretreatment of biomass materials

[0053] Gas explosion pretreatment with grain amaranth as raw material of biomass material: ① cut the dried material into 7cm segments, spray distilled water to make the material humidity reach 50% (that is, the wet weight of the material is equal to twice the dry weight); Put the shortened and sprayed materials into a gas explosion reactor with a capacity of 5L, and treat them at 225°C and 2.5MPa for 8 minutes; ℃ drying to constant weight, that is, the biom...

Embodiment 2

[0059] Same as Example 1, the only difference is that the pretreatment process of the biomass material is 10% CaO treatment, and the specific process is: ① after the material is dried, it is pulverized by a powder sample machine and passed through a 40-mesh sieve; ② Weigh 0.300g of biomass powder in Add CaO powder (CaO / biomass powder = 10%, w / w) and water to a 15mL centrifuge tube, centrifuge for 2 hours, and repeat each sample three times; , neutralize at 150r / min for 2h, centrifuge, discard the supernatant; ④ add distilled water to wash, centrifuge for 5min, discard the supernatant. Repeat the washing of the residue 6 times, and check the pH at the end to ensure that the washing is neutral; the residue is finally washed with acetic acid buffer, washed once with 10mL 0.2mol / L acetic acid buffer with pH 4.8, and waits for the enzymatic hydrolysis reaction.

Embodiment 3

[0061] In this embodiment, rice is used as the biomass material raw material, and all the other steps are the same as in Example 1, except that the gas explosion time is 16 minutes.

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Abstract

The invention discloses a method for promoting alcohol production of a biomass material. The method comprises the following steps of (1) collecting the biomass material, and pretreating the biomass material to obtain a pretreated biomass material; (2) washing the pretreated biomass material with water until the biomass material is neutral, carrying out enzymolysis, and adding amaranthus hypochondriacus protein (AP) to obtain AP-added enzymatic hydrolysate; and (3) directly applying the AP-added enzymatic hydrolysate to ethanol fermentation, adding yeast, and carrying out fermentation to produce alcohol. According to the method, the enzymolysis efficiency and the sugar alcohol conversion rate can be remarkably improved, and the ethanol yield is increased.

Description

technical field [0001] The invention relates to the technical field of producing cellulose ethanol, in particular to a method for promoting alcohol production from biomass materials. Background technique [0002] The utilization of lignocellulose as bioenergy is a systematic project. In order to improve the conversion efficiency and reduce the cost, breakthroughs are needed in all technical aspects of its fermentation alcohol production, including fully improving the enzymatic hydrolysis efficiency of lignocellulose, reducing fermentation inhibitors and Increase sugar alcohol conversion efficiency, reduce pretreatment cost and reduce secondary pollution in pretreatment. Dosing additives (such as surfactants, etc.) in the enzymatic hydrolysis process is one of the effective ways to improve enzymatic hydrolysis efficiency and sugar alcohol conversion efficiency. Some progress has been made in this area, but the following problems still exist in the selection of additives to ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/645
CPCC12P7/10Y02E50/10
Inventor 彭良才王友梅王艳婷涂媛媛夏涛汤逊文丰胜求山姆徐成宝余华
Owner HUAZHONG AGRI UNIV
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