Production strain of trans-L-hydroxyproline as well as construction method and application of production strain

A technology for producing hydroxyproline and bacterial strains, which is applied in the biological field and can solve the problems of further improvement in yield, cumbersome steps, and high price
CN111484997AActive Publication Date: 2020-08-04TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2020-08-04

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Abstract

The invention discloses a construction method of a trans-L-hydroxyproline production strain. The method comprises the step of enhancing the activity of a transport protein encoded by a nucleotide sequence shown in SEQ ID NO: 1, 2 or 3 in the trans-L-hydroxyproline production strain. The invention also discloses a trans-L-hydroxyproline production strain constructed by using the method and a methodfor preparing the trans-L-hydroxyproline by using the strain. The trans-L-hydroxyproline yield of the constructed trans-L-hydroxyproline producing strain is remarkably increased, and a new thought isdeveloped for constructing the trans-L-hydroxyproline producing strain.
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Description

technical field

[0001] The present invention relates to the field of biotechnology. Specifically, the present invention relates to a trans-L-hydroxyproline producing strain, a construction method and application of the producing strain. Background technique

[0002] Trans-L-hydroxyproline is the main amino acid in collagen and widely exists in nature as a constituent of elastin. As a naturally occurring trans-L-hydroxyproline, according to the position of the hydroxyl group, it is divided into trans-4-hydroxy-L-proline and trans-3-hydroxy-L-proline. Trans-4-hydroxy-L-proline is ubiquitous in nature and is widely used in fields such as medicine, food additives, animal feed and beauty industry. Due to its high price, it is mainly used for the synthesis of carbapenems (third generation, the last line of defense of antibiotics) side chains of antibiotics. The third-generation antibiotics are a type of atypical β-lactam antibiotics with the broadest antibacterial spectrum. The...

Claims

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