Method for determining content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos by LC-MS/MS

A technology of flavonoid aglycone and honeysuckle, which is applied in the direction of measuring devices, disease resistance to vector-borne diseases, instruments, etc., can solve the problem of low recovery rate of extraction of flavonoid aglycone

Active Publication Date: 2020-08-07
杭州海关技术中心
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Problems solved by technology

[0008] In order to solve the technical problem of low recovery rate of flavonoid aglycone extracted by hydrochloric acid hydrolysis, the purpose of this application is to provide a method for LC-MS / MS determination of various flavonoid aglycone content in Flos Lonicerae and / or Flos Lonicerae, the method adopts T-butyl-1,4-phenol was added to the sample as an antioxidant during extraction, and the study found that the loss rate of each aglycon during hydrolysis was less than 20%

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  • Method for determining content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos by LC-MS/MS
  • Method for determining content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos by LC-MS/MS
  • Method for determining content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos by LC-MS/MS

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Embodiment Construction

[0055] 1 Experimental part

[0056] 1.1 Instruments, reagents and materials

[0057] API 4000 type triple quadrupole tandem mass spectrometer (ESI with electrospray ion source, AB company, the United States), 1100 type liquid chromatograph (Agilent company, the United States), Milli-Q Synergy 185 ultrapure water device (Millipore company, The U.S.), IKA MS3 Basic vortexer (IKA company, Germany), 24-hole solid phase extraction device (Supelco company, U.S.), Heraeus Multifuge X1R desktop centrifuge (Thermo company, U.S.), G&G JJ500 electronic balance ( Shuangjie company product, China), Mettler AE260 (Mettler Toledo company product, Switzerland).

[0058] Methanol (chromatographically pure, TEDIA Company), formic acid (mass spectrometry grade, Scharlau Company), acetonitrile (chromatographically pure, Scharlau Company), ethanol (analytical pure, Anhui Ante Food Co., Ltd.), hydrochloric acid (superior alcohol, Yonghua Chemical Technology (Jiangsu) Co., Ltd.), T-butyl-1,4-phenol ...

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Abstract

The invention relates to a method for simultaneously determining the content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos, in particular to a method for hydrolyzing flavonoidglycosides and determining the content of various flavonoid aglycones in lonicerae flos and/or lonicerae flos through solid-phase extraction purification and LC-MS/MS. The method comprises the stepsof extraction, purification and determination, wherein a hydrochloric acid ethanol solution hydrolysis extraction method is adopted for extraction, the multiple flavonoid aglycones are quercetin, luteolin, kaempferol, apigenin and baicalein, and T-butyl-1, 4-phenol is added into a sample to serve as an antioxidant during extraction. According to the method, T-butyl-1, 4-phenol is added into a sample as an antioxidant during extraction, and research finds that the loss rate of each aglycone in the hydrolysis process is less than 20%. The method has the characteristics of simplicity, convenience, rapidness, low resource consumption and low detection cost.

Description

technical field [0001] The invention relates to a method for simultaneously determining the content of multiple flavonoid aglycones in Flos Lonicerae and / or Flos Lonicerae, in particular to a hydrolyzed flavonoid glycoside, which is purified by solid phase extraction, and determined by LC-MS / MS for Flos Lonicerae and / or Flos Lonicerae Methods for the content of various flavonoid aglycones in Background technique [0002] Mountain silver flower is Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq, Lonicera confusa DC. or Lonicera fullvotomentosa Hsu et S.C.Cheng. Flower, honeysuckle is the dry flower bud or the first flower of Lonicera japonica Thunb. Mountain silver flower and honeysuckle need to be harvested before the flowers bloom in early summer. They have the effect of clearing away heat and detoxifying, and evacuating wind-heat. Fever. Studies have also found that it has antibacterial, antiviral, antioxidative and antiatherosclerotic effects. [0003] Ori...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/02G01N30/72G01N30/86
CPCG01N30/06G01N30/14G01N30/02G01N30/72G01N30/86G01N2030/067G01N2030/062G01N2030/047G01N2030/146Y02A50/30
Inventor 侯建波谢文史颖珠姚滨滨陆顺祝泽龙张雅琴
Owner 杭州海关技术中心
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