A kind of imprinted electrochemical sensor for Listeria monocytogenes and its preparation method
A Listeria monocytogenes, electrochemical technology, applied in the field of Listeria monocytogenes imprinted electrochemical sensor and its preparation, can solve the problems of long detection time, high antibody preparation cost, and inability to batch detection, etc., to achieve Good electrical conductivity and biocompatibility, fast detection speed, and the effect of improving detection sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0065] The preparation of embodiment 1 sensor
[0066] A method for preparing Listeria monocytogenes imprinted electrochemical sensor, the preparation process is as follows figure 1 shown, including the following steps:
[0067] (1) Place the ITO electrode in 5 mM chloroauric acid solution, use cyclic voltammetry, the initial potential is -1 V, the final potential is 0.5 V, the scanning rate is 100 mV / s, and the number of scanning cycles is 15 cycles. Electrodeposition of gold nanoparticles;
[0068] (2) Wash the electrode after gold electrodeposition with deionized water, and dry it at room temperature; configure the electropolymerization solution in which the template Listeria monocytogenes and the functional monomer pyrrole are dissolved in the electrolyte potassium chloride aqueous solution (the monocytogenes in the electropolymerization solution The concentration of Listeria ATCC19115 is 1.0×10 8 CFU / mL; the concentration of pyrrole monomer is 0.2 M; the concentration...
Embodiment 2
[0073] Example 2 ITO electrode surface modification
[0074] 1. Optimization of the number of scan turns in step (1)
[0075] The number of scanning circles in step (1) was adjusted to 5-15 circles, and other parameters were kept the same as those in Example 1 to obtain an ITO electrode modified with gold nanoparticles.
[0076] figure 2 A is a graph of 15 cycles of cyclic voltammetry scan. It can be seen from the figure that a reduction peak was observed at E=0.19 V when scanning the first cycle; when scanning the second and third cycles, this reduction peak moved to the negative potential; In the number, this reduction peak continuously moves to the positive potential, this is because the gold nanoparticles deposited on the surface of the ITO electrode promote the reduction of chloroauric acid more and more easily, resulting in the gold nanoparticles obtained in the second scan cycle compared with the previous scan. The circle is easier, and the potential of deposition ...
Embodiment 3
[0091] Example 3: Characterization of Sensors
[0092] (1) Electrochemical characterization
[0093] The parameter conditions of cyclic voltammetry are as follows: the initial potential is -0.2 V, the final potential is +0.6 V, the scanning speed is 100 mV / s, and the sampling interval is 1 mV; the parameter conditions of differential pulse voltammetry are as follows: the initial potential is -0.2 V , the final potential is +0.6V, the incremental potential is 5 mV, the pulse width is 50 ms, and the pulse period is 500 ms; the parameters of the AC impedance method are as follows: the initial potential is 0.2 V, the minimum frequency is 0.1 Hz, and the maximum frequency is 10 4 Hz with an amplitude of 5mV. The potassium ferricyanide electrolyte concentration was 2.5 mM.
[0094] exist Figure 7 A and Figure 7 In B, curve a is the redox peak obtained by scanning the bare ITO electrode in potassium ferricyanide solution, and the peak current at this time is 42.15 µA (by Fi...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More