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Black phosphorus nanosheet/gold nanoparticle composite material and preparation method and application thereof

A gold nanoparticle and composite material technology, which is applied in the field of black phosphorus nanosheet/gold nanoparticle composite material and its preparation to achieve the effect of high-efficiency loading

Inactive Publication Date: 2020-08-14
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, concerns about the safety and biocompatibility of these nanomaterials remain

Method used

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  • Black phosphorus nanosheet/gold nanoparticle composite material and preparation method and application thereof
  • Black phosphorus nanosheet/gold nanoparticle composite material and preparation method and application thereof
  • Black phosphorus nanosheet/gold nanoparticle composite material and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of black phosphorus nanosheets:

[0050]Disperse 5.0 mg of black phosphorus powder in 30.0 mL of N-methylpyrrolidone solution and sonicate in an ice bath for 11 h. After centrifugation at 2,000 rpm for 5 min to remove remaining unexfoliated particles in the mixture, the supernatant was collected, then centrifuged at 10,000 rpm for 5 min, and washed twice with ultrapure water to obtain a brown suspension of black phosphorus nanosheets. Take a small amount of the solution and observe it under a scanning electron microscope. figure 1 A. figure 1 B shows the black phosphorus nanosheet structure.

Embodiment 2

[0052] Preparation of gold nanoparticles:

[0053] When 99.0 mL of ultrapure water was heated to 60 °C in the round bottom flask, 1.0 mL of 1 wt.% chloroauric acid solution was added immediately. When the mixed solution starts to boil, add 4.0 mL of trisodium citrate solution. After reacting for 12 minutes, it was naturally cooled to room temperature to obtain a gold nanoparticle dispersion. Take a small amount of the solution and observe it under a scanning electron microscope. figure 1 C. figure 1 D shows the gold nanoparticle structure.

Embodiment 3

[0055] The inhibitory ability of each component to tongue squamous cell carcinoma cell SCC-9 was detected when each composite material was used as a drug carrier:

[0056] Extract, digest and count SCC-9 cells in the logarithmic growth phase, and divide into 5×10 4 Cells / mL were seeded into 96-well plates at 100 μL per well. Five replicate wells were designed for each group. After the cells adhered, they were treated with different drug-containing media (50 μg / mL black phosphorus nanosheet dispersion (BPNSs), 50 μg / mL gold nanoparticle dispersion (AuNPs), 2 mg / mL cisplatin solution (CDDP) and ultrapure water (DDW)) for 4 hours. After removal of the drug-containing medium, 100 μL of medium (containing 10 μL of LCCK-8 reagent) was added to each well. at 37°C and 5% CO 2 After 4 hours of incubation under high temperature, analyze the optical density (OD) value at 450nm place by Microplate Reader, get as follows figure 2 , The distribution of optical density values ​​shown i...

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Abstract

The invention discloses a black phosphorus nanosheet / gold nanoparticle composite material and a preparation method and application thereof. In the black phosphorus nanosheet / gold nanoparticle composite material, the mass percentage of the black phosphorus nanosheet is greater than or equal to 16.7%, a gold nanoparticle solution is added into dispersion liquid including the black phosphorus nanosheet, and thus the black phosphorus nanosheet / gold nanoparticle composite material can be obtained through ultrasonic oscillation. The black phosphorus nanosheet / gold nanoparticle composite material provided by the invention can efficiently load cis-platinum chemotherapeutic drugs, can realize photo-thermal, photodynamic and chemotherapeutic combined treatment on oral squamous cell carcinoma, and has excellent treatment effect.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a black phosphorus nanosheet / gold nanoparticle composite material and its preparation method and application. Background technique [0002] Head and neck squamous cell carcinoma is the sixth most common cancer type in the world. Among them, oral squamous cell carcinoma (OSCC) accounts for approximately 90% of all oral cancer cases and is the most common primary tumor diagnosed in the oral cavity. Currently, the treatment of OSCC is mainly based on the stage of cancer progression. Early stage cancers (stages I and II) are essentially curable with surgery or radiation therapy. However, even combining surgery, radiotherapy, and chemotherapy, it is difficult to obtain satisfactory results in the treatment of advanced OSCC (stages III and IV). Unfortunately, a considerable proportion of OSCC patients may develop local invasion and metastasis, resulting in the 5-year survival rat...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/02A61K47/04A61K41/00A61K33/243A61P35/00
CPCA61K9/5115A61K41/0052A61K41/0057A61P35/00A61K33/243A61K2300/00
Inventor 曾俊杰唐瞻贵曾冬铭
Owner CENT SOUTH UNIV
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