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Application of nanoparticles as tumor microenvironment responsive drug or imaging agent

A tumor microenvironment and nanoparticle technology, which is applied in the application field of nanoparticles as tumor microenvironment responsive drugs or imaging agents, can solve the problem of inability to realize real-time monitoring of chemotherapy drugs, inability to realize fixed-point release of chemotherapy drugs, and not to mention chemotherapy drugs. Control of release rate and other issues to prevent premature inactivation, increase cycle time, and reduce toxicity

Inactive Publication Date: 2020-08-14
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the solutions in the prior art have the following defects: after the nanoparticles in the technical solution enter the biological tissue, real-time monitoring of the tissue distribution and aggregation of the chemotherapy drugs cannot be realized, and the fixed-point release of the chemotherapy drugs at the tumor tissue cannot be realized. , not to mention the control of the release rate of chemotherapy drugs

Method used

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  • Application of nanoparticles as tumor microenvironment responsive drug or imaging agent
  • Application of nanoparticles as tumor microenvironment responsive drug or imaging agent
  • Application of nanoparticles as tumor microenvironment responsive drug or imaging agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the preparation of nanoparticle

[0063] 10 mg of DOX·HCl (dobiraxin hydrochloride) was dissolved in 10 ml of DMSO (dimethyl sulfoxide) solution. Then stir moderately at room temperature, add 1ml of trimethylamine, remove hydrochloric acid to convert hydrophilic DOX·HCl into hydrophobic DOX nanoparticles, and obtain a mixture after uniform stirring. Then 100 μl of the prepared mixture (DOX / DMSO solution neutralized by trimethylamine) was dripped into 980 μl of high-purity water under vigorous stirring (stirring at a speed of 700 rpm), and the dropping rate of high-purity water was 800 μl / min to obtain a dispersion. Then 10 μl of Ta (tannic acid) solution (40 mg / ml) and 10 μl of FeCl 3 ·6H 2 O solution (20 mg / ml) was sequentially added to the above dispersion, then sonicated for 50 s, and neutralized with 1 μM NaOH solution. The resulting product was centrifuged with deionized water to remove excess TA and FeCl 3 . The nanoparticles were redispersed in...

Embodiment 2

[0066] 10 mg of DOX·HCl (dobiraxin hydrochloride) was dissolved in 10 ml of DMSO (dimethyl sulfoxide) solution. After moderate stirring at room temperature, 1 ml of trimethylamine was added, and hydrochloric acid was removed to convert hydrophilic DOX·HCl into hydrophobic DOX nanoparticles. Then, 100 μl of the prepared DOX / DMSO solution was dripped into 980 μl of high-purity water under vigorous stirring (stirring at a speed of 1000 rpm), and the dropping speed of the high-purity water was 1000 μl / min to obtain a dispersion. Then 100 μl of Ta (tannic acid) solution (40 mg / ml) and 100 μl of FeCl 3 ·6H 2 O solution (20 mg / ml) was sequentially added to the above dispersion, then sonicated for 50 s, and neutralized with 1 μM NaOH solution. The resulting product was centrifuged with deionized water to remove excess TA and FeCl 3 . The nanoparticles were redispersed in high-purity water to obtain DFTNPs.

Embodiment 3

[0068] 10 mg of DOX·HCl (dobiraxin hydrochloride) was dissolved in 10 ml of DMSO (dimethyl sulfoxide) solution. After moderate stirring at room temperature, 1 ml of trimethylamine was added, and hydrochloric acid was removed to convert hydrophilic DOX·HCl into hydrophobic DOX nanoparticles. Then, 100 μl of the prepared DOX / DMSO solution was dripped into 980 μl of high-purity water under vigorous stirring (stirring at a speed of 300 rpm), and the dropping rate of the high-purity water was 100 μl / min to obtain a dispersion. Then 50 μl of Ta (tannic acid) solution (40 mg / ml) and 50 μl of FeCl 3 ·6H 2 O solution (20 mg / ml) was sequentially added to the above dispersion, then sonicated for 50 s, and neutralized with 1 μM NaOH solution. The resulting product was centrifuged with deionized water to remove excess TA and FeCl 3 . The nanoparticles were redispersed in high-purity water to obtain DFTNPs.

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Abstract

The invention belongs to the field of biological medicines, and relates to application of nanoparticles, in particular to application of the nanoparticles as tumor microenvironment responsive drugs orimaging agents. The nanoparticle comprises a chelate shell formed by tannic acid and ferric ions, and chemotherapeutic drugs are entrapped in the chelate shell. In a tumor microenvironment, the nanoparticle can slowly release a chemotherapeutic drug entrapped in the nanoparticle and slowly release iron ions in the nanoparticle to enhance the nuclear magnetic resonance imaging effect. The technical scheme can be applied to medical practice of tumor imaging and tumor treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of a nanoparticle, in particular to the application of a nanoparticle as a tumor microenvironment-responsive drug or an imaging agent. Background technique [0002] Chemotherapy is a classic and effective tumor treatment technology, which has been widely used in clinic. However, chemotherapeutic drugs lack targeting, and chemotherapeutic drugs are easily swallowed and absorbed in non-lesional areas; and some chemotherapeutic drugs have a short half-life in vivo, and are easy to be inactivated during in vivo transport; in addition, the sudden release of chemotherapeutic drugs will also increase its systemic toxicity. The above shortcomings hinder the clinical application of chemotherapy. Therefore, more and more researchers and clinicians are devoting themselves to the study of encapsulating chemotherapy drugs into nanoparticle drug delivery systems. Rationally designed n...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/22A61K47/02A61K31/704A61K41/00A61P35/00A61K49/10A61K49/22A61K49/18A61K49/00
CPCA61K9/5115A61K9/5123A61K9/5192A61K31/704A61K41/0052A61K49/00A61K49/0002A61K49/10A61K49/1845A61K49/225A61P35/00A61K2300/00
Inventor 任建丽周志益罗远利乔斌王志刚冉海涛李攀郝兰曹阳
Owner CHONGQING MEDICAL UNIVERSITY
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