Fluorescent probe kit for detecting total mercury and methyl mercury in water and detection method

A technology of fluorescent probes and detection methods, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problem of fewer fluorescent probes, and achieve the effect of strong affinity

Inactive Publication Date: 2020-08-14
DATANG ENVIRONMENT IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few fluorescent probes that can effectively detect and distinguish mercury ions from methylmercury

Method used

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  • Fluorescent probe kit for detecting total mercury and methyl mercury in water and detection method
  • Fluorescent probe kit for detecting total mercury and methyl mercury in water and detection method
  • Fluorescent probe kit for detecting total mercury and methyl mercury in water and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] S11. Use a pipette gun to draw 2.5mL of acetonitrile-HEPES buffer solution and 2.5mL of standard water sample BY-1 into the cuvette, mix well and irradiate with an ultraviolet lamp with a wavelength of 365nm in a dark place to obtain a reference;

[0051] S12. Add 0.1mL of probe solution to the above reference, mix well and irradiate again in the dark with a UV lamp with a wavelength of 365nm. It can be observed that there is blue fluorescence visible to the naked eye in the cuvette;

[0052] S13. Continue to add 4 μL of DNA solution to the reference in step S12. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence in the cuvette disappears, and the solution appears similar to the reference. traits.

[0053] It can be seen from the above test phenomena that the standard water sample BY-1 only contains mercury ions.

Embodiment 2

[0055] S21. Use a pipette gun to draw 2.0mL of acetonitrile-HEPES buffer solution and 3.0mL of standard water sample BY-2 into the cuvette, mix well and irradiate in the dark with an ultraviolet lamp with a wavelength of 365nm to obtain a reference;

[0056] S22. Add 0.1 mL of probe solution to the above reference, mix well and irradiate again in a dark place with an ultraviolet lamp with a wavelength of 365 nm, and it can be observed that there is blue fluorescence visible to the naked eye in the cuvette;

[0057] S23. Continue to add 5 μL of DNA solution to the reference in step S22. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence in the cuvette does not disappear, and there is no change in intensity.

[0058] It can be seen from the above test phenomena that the standard water sample BY-2 only contains methylmercury.

Embodiment 3

[0060] S31. Use a pipette gun to draw 3.0 mL of acetonitrile-HEPES buffer solution and 2.0 mL of standard water sample BY-3 into the cuvette, mix well and irradiate in the dark with an ultraviolet lamp with a wavelength of 365 nm to obtain a reference;

[0061] S32. Add 0.1 mL of the probe solution to the above reference, mix well and irradiate again in the dark with an ultraviolet lamp with a wavelength of 365 nm, and it can be observed that there is blue fluorescence visible to the naked eye in the cuvette;

[0062] S33. Continue to add 5 μL of DNA solution to the reference in step S32. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence intensity in the cuvette decreases, but it is not completely quenched. , indicating that the standard water sample BY-3 contains mercury ions and some methylmercury. In order to ensure that the fluorescence of mercury ions can be completely quenche...

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Abstract

The invention relates to the technical field of total mercury and methyl mercury detection, in particular to a fluorescent probe kit for detecting total mercury and methyl mercury in water and a detection method. The fluorescent probe kit comprises a buffer solution reagent, a probe solution and a DNA reagent. The buffer solution is an acetonitrile-HEPES buffer solution, the concentration of the buffer solution is 8-12 mM, and the pH value of the buffer solution is 6-8; a pyridine structure, four naphthalimide fluorophores and an oxygen-containing long-chain structure are contained in a probemolecule; the base sequence of the DNA is 5-TTGTTTGTTGGCCCCCCTTCTTTCTT-3'. Free rotation of four naphthalimide fluorophores in the probe can annihilate excitation energy in a non-radiative manner, sothat the probe cannot be excited to emit strong fluorescence in a solution; when mercury ions or methyl mercury exists in the solution, mercury ions or methyl mercury can be coordinated and combined with the probe to limit the rotation of a naphthalimide group, a probe solution can be excited by ultraviolet light to generate strong fluorescence, and DNA can be used as a mercury ion masking agent for qualitative detection of methyl mercury when the mercury ions and the methyl mercury exist at the same time.

Description

technical field [0001] The invention relates to the technical field of total mercury and methylmercury detection, in particular to a fluorescent probe kit and a detection method for detecting total mercury and methylmercury in water. Background technique [0002] During the mining, smelting and processing of heavy metals, various heavy metals such as lead, mercury, cadmium and cobalt enter the water body and cause serious environmental pollution. Among them, the pollution caused by mercury is the most common one. Mercury in the water environment can accumulate in algae and sediment, be absorbed by fish and shellfish, and enrich through the food chain, thereby posing a risk of harming human health. The toxicity of mercury in water depends not only on its concentration, but also on its valence and form. Among them, the toxicity of methylmercury is much greater than that of mercury ions, and mercury ions can also be converted into methylmercury under certain conditions. Compar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06
CPCC09K11/06C09K2211/1029G01N21/643G01N2021/6432
Inventor 杨言刘海洋白玉勇谷小兵李飞麻晓越荆亚超高阳彭思伟
Owner DATANG ENVIRONMENT IND GRP
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