Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v protein antibody

An African swine fever virus and double-antigen sandwich technology is applied in the field of veterinary biological products to achieve the effects of small variation coefficient, high detection sensitivity and high precision

Pending Publication Date: 2020-08-14
广州敏捷生物技术有限公司
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no commercial vaccine for the disease. The prevention and control of the epidemic mainly relies on measures such as strengthening the biosecurity management of farms, restricting the cross-regional flow of pigs and pig products, and culling all pigs in the epidemic site and in the epidemic area.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v protein antibody
  • Double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v protein antibody
  • Double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v protein antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v antibody provided by the present invention, see figure 1 , including a box body A, the box body A is equipped with a CD2v antibody immunofluorescence chromatography detection card B and a serum diluent bottle C with a serum diluent, a serum bottle D with a standard positive serum, and a standard positive serum bottle D with a standard Serum bottle E of negative serum; the CD2v antibody immunofluorescence chromatography detection card B includes a PVC bottom plate 1, and the PVC bottom plate 1 is provided with an absorption pad 2, a reaction membrane 3, a binding pad 4 and The sample pad 5, the binding pad 4 is loaded with fluorescently labeled CD2v protein and fluorescently labeled goat anti-chicken IgY, the reaction membrane 3 is provided with a detection line T and a quality control line C, the detection Line T is coated with recombinant CD2v protein, and the q...

Embodiment 2

[0046] The preparation method of the above-mentioned kit includes the following steps: flatly lay the PVC bottom plate 1 with NC film on the work surface, paste the binding pad 4, the sample pad 5 and the absorption pad 2 respectively, and cut them into 4.0mm wide strips with a strip cutter. For test strips, put each test strip into a plastic card case, place each reagent card in an aluminum film bag, add 1 pack of 1g desiccant, heat seal the seal, and it is an immunofluorescence chromatography test card.

[0047] Specifically:

[0048] 1) The coated film is made by the following method:

[0049] Dilute CD2v protein and IgY with 3% sucrose-containing PB (0.01M PH7.4) to a concentration of 1 mg / ml as the test line and quality control line solutions, draw a line on the nitrocellulose membrane, and use a marker pen at one end of the membrane Mark the quality control line and detection line. The distance between the quality control line and the detection line is 5 mm, the distanc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a double-antigen sandwich immunofluorescence chromatography kit for detecting an African swine fever virus CD2v protein antibody. The invention aims to provide a double-antigensandwich method immunofluorescence chromatography detection reagent for detecting the African swine fever virus CD2v protein antibody, which is used for screening a large number of African swine fever virus antibodies and identifying infection of African swine fever wild strains in CD2v gene deletion strain vaccine immune pigs in future. According to the technical scheme, the kit comprises a kitbody, and a CD2v protein antibody immunofluorescence chromatography detection card, a serum diluent bottle filled with a serum diluent, a standard positive serum bottle filled with standard positive serum and a standard negative serum bottle filled with standard negative serum are arranged in the kit body; the CD2v protein antibody immunofluorescence chromatography detection card comprises a PVC bottom plate, an absorption pad, a reaction film, a conjugate pad and a sample pad are sequentially arranged on the PVC bottom plate from left to right, the conjugate pad is loaded with fluorescently-labeled CD2v protein and fluorescently-labeled goat anti-chicken IgY, the reaction film is provided with a detection line T and a quality control line C, the detection line T is coated with recombinantCD2v protein, and the quality control line C is coated with goat anti-chicken IgY immunoglobulin.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a double-antigen sandwich immunofluorescence chromatography kit for detecting African swine fever virus CD2v protein antibody. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, febrile, highly contagious infectious disease of pigs caused by African swine fever virus (ASFV) infecting domestic pigs and wild boars. The main features are waste, skin cyanosis, visceral parenchymal organ hemorrhage, and respiratory system and nervous system dysfunction. The disease has a very short course and a high mortality rate. [0003] There are many genotypes of African swine fever virus, and 24 genotypes are currently known. So far, there is no commercial vaccine for the disease. The prevention and control of the epidemic mainly relies on measures such as strengthening the biosecurity management of farms, restricting th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558
CPCG01N33/6854G01N33/582G01N33/585G01N33/56983G01N33/558G01N2469/20G01N2333/01
Inventor 汤永平蒋智勇羊晓珊梁展鹏刘卫李闯
Owner 广州敏捷生物技术有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com