Application of icariin in preparation of medicine for preventing and treating Alzheimer's disease

A technology for Alzheimer's disease and icariin, applied in the field of medicine, can solve problems such as the incomplete understanding of the molecular mechanism of the nervous system, and achieve the effects of reducing deposition, increasing the ratio, and inhibiting neuron apoptosis

Inactive Publication Date: 2020-08-18
ZUNYI NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exact molecular mechanism of icariin on the nervous system is still not fully understood

Method used

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  • Application of icariin in preparation of medicine for preventing and treating Alzheimer's disease
  • Application of icariin in preparation of medicine for preventing and treating Alzheimer's disease
  • Application of icariin in preparation of medicine for preventing and treating Alzheimer's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1Morris water maze experiment

[0031] The Morris water maze test was used to evaluate the spatial learning and memory abilities of the mice. The Morris water maze consists of a black circular pool (120 cm in diameter and 50 cm in depth) divided into four quadrants. The water temperature was maintained at 23±1°C and made opaque by adding titanium dioxide. The camera is placed about 2m above the center of the pool to automatically collect images of animal swimming trajectories, and the collected signals are directly input into the computer, which is automatically analyzed and processed by the automatic collection and analysis system that comes with the device.

[0032] The Morris water maze experiment consists of two parts, the hidden platform test and the space search test. The concealed platform test was carried out from the 18th day to the 21st day after ICA administration. A platform was placed in the third quadrant, and the animal was gently put into th...

Embodiment 2

[0033] Example 2 Hematoxylin-eosin (HE) staining

[0034] For hematoxylin-eosin (HE) staining, brain tissue was fixed in paraformaldehyde for one week, sectioned through the hippocampus and immersed in paraformaldehyde. Then, the paraffin samples were made into a series of slices with a thickness of 4 μm, and placed in a water bath to fully expand. The unfolded slices were placed at 60°C for 4 hours for fixation. Then dewax with xylene, dehydrate with graded alcohol, stain with hematoxylin for 1 minute, rinse with tap water, place in 1% hydrochloric acid alcohol differentiation solution for 5 seconds, treat with 1% ammonia water, and stain with eosin. After the sections were dehydrated with graded alcohol, they became transparent in xylene and sealed in neutral glue. Histopathological changes were observed with an Olympus microscope (BX60; Olympus, Japan), and the experiment was repeated three times.

Embodiment 3

[0035] Example 3 Immunohistochemical Analysis

[0036] Immunohistochemistry was performed according to standard procedures. Briefly, sections of the hippocampus from each mouse were sectioned in parallel, cut into 6 μm thick sections, and mounted onto silane-coated glass slides. Sections were boiled in 0.01 M citrate buffer (pH 6.0) for 10 minutes and then rinsed with PBS. Endogenous peroxidase was blocked with methanol containing 0.3% hydrogen peroxide for 20 min, blocked with 5% goat serum and 0.3% TritonX-100 in PBS, and then blocked with primary antibody Aβ1-42 (1 :200) overnight, then washed and incubated with anti-rabbit secondary antibody (1:10000) for 1 hour. Cells were observed with a confocal microscope (FV1000, Olympus, Japan). Six to 10 slices of the hippocampus of each mouse were selected for imaging and analysis.

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Abstract

The invention relates to the application of icariin in the preparation of a medicine for preventing and treating Alzheimer's disease. According to the invention, experiments such as a Morris water maze experiment, a Western blot experiment, an HE staining method and the like are carried out in an SAMP8 model mouse; it is proved that learning and memory disorder of SAMP8 mice can be reversed afterintragastric administration of ICA, icariin can down-regulate expression of BACE1 so as to reduce deposition of cytotoxic A beta 1-42. Besides, icariin can improve cognitive defects by increasing theexpression of anti-apoptotic protein Bcl-2, reducing the expression of pro-apoptotic protein Bax and remarkably increasing the ratio of Bcl-2 to Bax, so that neuronal apoptosis is inhibited, and neurons are protected. The invention provides further evidences for the clinical curative effect of icariin on Alzheimer's disease.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of icariin in the preparation of drugs for preventing and treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD), the leading cause of dementia, is a degenerative disease of the central nervous system characterized by progressive deterioration of memory and other cognitive functions. As the global population ages and the incidence of AD increases rapidly, the economic burden of dementia on social healthcare systems and families is expected to exceed the combined burden of cancer, heart disease, and stroke. Despite extensive research to understand its pathogenesis, there are no effective treatments to prevent, arrest or reverse the pathological features of AD. Therefore, finding effective drugs for treating AD is an important and urgent task worldwide. [0003] Icariin (Icariin, ICA, C 33 h 40 o 15 , molecular weight:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P25/28
CPCA61K31/7048A61P25/28
Inventor 罗勇吴杰曲璟秋冯飞李园园黄南渠巴智胜施尚鹏邓成敏田鹏吴德静罗秀梅李莹周彦均周永健
Owner ZUNYI NO 1 PEOPLES HOSPITAL
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