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Method for purifying ARA crude oil

A purification method and crude oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fat oil/fat production, etc., can solve the problems of high deodorization temperature, polluted environment, long time of high temperature treatment, etc.

Pending Publication Date: 2020-08-21
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are inevitably some problems in this process technology, such as: in order to meet the requirement of low acid value in alkali refining, excessive alkali is usually added, and some triglycerides will inevitably be saponified; high COD wastewater produced by alkali refining will pollute the environment; Alkali refining requires high temperature treatment for a long time, which will easily cause the peroxide value and anisidine value of the product to increase; high deodorization temperature and long time will easily produce trans fatty acids and other disadvantages

Method used

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  • Method for purifying ARA crude oil
  • Method for purifying ARA crude oil
  • Method for purifying ARA crude oil

Examples

Experimental program
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Effect test

Embodiment 1

[0124]The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to ...

Embodiment 2

[0130] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...

Embodiment 3

[0136] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...

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Abstract

The invention belongs to the field of fermentation engineering, and relates to a method for purifying ARA crude oil, in particular to a method for purifying ARA crude oil, which comprises the steps ofhydration, decolorization and molecular distillation of ARA crude oil. The method for preparing ARA has the advantages of high yield and high purity, and is beneficial to large-scale industrial production of ARA.

Description

[0001] The present invention is a divisional application of the parent application with the application number 201710097797.5. The application date of the parent application is February 23, 2017, and the title of the invention is "A method for producing ARA by microbial fermentation". technical field [0002] The invention belongs to the field of fermentation engineering, and relates to a method for producing ARA by microbial fermentation, in particular to a method for producing mixed oil containing arachidonic acid (ARA) by industrialized fermentation of Mortierella alpina strains. Background technique [0003] ARA, also known as arachidonic acid, full name eicosadonic acid (cis-5,8,11,14-arachidonic acid, ARA), is a polyunsaturated fatty acid of the omega-6 series. ARA is an important essential fatty acid for the human body, and it is also a polyunsaturated fatty acid with the highest content and the widest distribution in the human body. ARA plays an important role as a p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/14C11B1/02C11B1/06C11B3/12C11B3/10C11B3/00C12R1/645C12P7/6427
CPCC11B1/025C11B1/06C11B3/001C11B3/008C11B3/10C11B3/12C12P7/6427C11B3/16
Inventor 吴轶陈必钦王炳荣王跃飞蒋四富徐鲁明
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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