Method for purifying ARA crude oil
A purification method and crude oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fat oil/fat production, etc., can solve the problems of high deodorization temperature, polluted environment, long time of high temperature treatment, etc.
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Embodiment 1
[0124]The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to ...
Embodiment 2
[0130] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...
Embodiment 3
[0136] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...
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