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Induction medium for cultivating and breeding Allium fistulosum, and induction culture method

A technology of induction medium and culture method, which is applied in the field of induction medium for cultivation of scallion flowers and induction culture of scallion anthers, which can solve instability, low anther callus induction rate, and slow progress in the research of scallion anther culture, etc. problem, to achieve good induction effect and improve the effect of callus induction rate

Inactive Publication Date: 2020-09-15
富阿丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on anther culture of scallion has been slow, and there are few reports on the breeding of scallion using this technology. The main reason is that the induction rate of anther callus is low and unstable, which directly limits the application of the technology of anther culture in scallion in breeding.

Method used

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  • Induction medium for cultivating and breeding Allium fistulosum, and induction culture method
  • Induction medium for cultivating and breeding Allium fistulosum, and induction culture method
  • Induction medium for cultivating and breeding Allium fistulosum, and induction culture method

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Experimental program
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Effect test

Embodiment 1

[0025] The experimental materials were Yishui scallion and Zhangqiu sycamore, the seeds were purchased from the market and planted in the field.

[0026] Carry out scallion anther induction culture according to the following methods:

[0027] 1) Material collection and pretreatment: collect unopened or newly opened scallion flower buds in the morning in fine weather, take them to the laboratory in an ice box, and select flower buds whose pollen development period is the single-nucleus marginalization stage under a microscope, moisten them with water, and put them into the mat In the petri dish with test paper, pretreat at 4°C for 2-3 days. The specific method of microscopic examination is: peel off the anthers from the flower buds, place them on a glass slide, add a small drop of carbo fuchsin staining solution, crush the anthers with tweezers, squeeze out the small spores, remove the residue, and then Cover the slides and place them under a microscope to observe the developm...

Embodiment 2

[0045] The influence of embodiment 2 different induction mediums on the callus induction rate of scallion anthers

[0046] Induction medium of the present invention: KNO 3 1600mg / L, CaCl 2 2H 2 O 325mg / L, (NH 4 ) 2 SO 4 140mg / L, NaH 2 PO 4 ·H 2 O 115mg / L, MgSO 4 ·7H 2 O 175mg / L, MnSO 4 4H 2 O 5.5mg / L, ZnSO 4 ·7H 2 O 2.0mg / L, H 3 BO 3 6.0mg / L, KI 1.5mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L, Fe-EDDHA 28.3mg / L, corn silk extract 40ml / L, hydrolyzed casein 135mg / L, inositol 105mg / L, sodium pyruvate 18.4mg / L, fumaric acid 22.5mg / L, day Particic acid 3.0mg / L, glycine 2.0mg / L, sodium humate 10mg / L, riboflavin 0.3mg / L, vitamin B1 0.4mg / L, vitamin B6 0.6mg / L, D-biotin 0.9mg / L, choline 2.8mg / L, salicylic acid 0.1mg / L, sodium butyrate 10mmol / L, paclobutrazol 0.5mg / L, KT 0.5mg / L, 6-BA 1.0mg / L, p-hydroxybenzoic acid 1.0 g / L, sucrose 30g / L, maltose 30g / L, plant gel 7g / L, pH value 5.8.

[0047] Control induction medium:...

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Abstract

The invention provides an induction medium for cultivating and breeding Allium fistulosum. The formula of the culture medium comprises KNO3, CaCl2. 2H2O, (NH4) 2SO4, NaH2PO4.H2O, MgSO4. 7H2O, MnSO4. 4H2O, ZnSO4. 7H2O, H3BO3, KI, Na2MoO4. 2H2O, CuSO4. 5H2O, CoCl2. 6H2O, Fe-EDDHA, a corn stigma extracting solution, casein hydrolysate, inositol, sodium pyruvate, fumaric acid, aspartic acid, glycine,sodium humate, riboflavin, vitamin B1, vitamin B6, D-biotin, choline, salicylic acid, sodium butyrate, paclobutrazol, KT, 6-BA, p-hydroxybenzoic acid, sucrose, maltose and phytagel. The invention further provides an induction culture method of the allium fistulosum medicine, the allium fistulosum medicine is inoculated to the induction culture medium for culture after being uniform magnetic fieldtreatment, the callus induction efficiency of the allium fistulosum medicine can be remarkably improved, and establishment of an allium fistulosum medicine cultivating and breeding system is promoted.

Description

technical field [0001] The invention belongs to the high-tech field of vegetable cultivation, and in particular relates to an induction medium for cultivating green onion flower seeds and a method for inducing cultivation of green onion anthers. Background technique [0002] Scallions (scientific name: Allium. fistulosum L. var. gigantum Makino ) is a variant of the onion species of the genus Allium in the family Liliaceae. The leaves are cylindrical and hollow, and the stem of the leaf sheath is included as a pseudostem. It is a biennial or triennial herb and is a widely cultivated vegetable in my country. According to the literature, the origin of onion is Siberia, Northwest and Northeast China, and Central Asia. China has a long history of cultivating scallions. Due to its strong adaptability and wide cultivation area, a large number of local varieties have been formed. Scallion is a condiment that people often eat in four seasons, and it is also a nutritious vegetable....

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00
Inventor 富阿丽
Owner 富阿丽
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