Taq DNA polymerase monoclonal antibody combination, polymerase reaction system comprising it, and application thereof

A monoclonal antibody and reaction system technology, applied in the direction of anti-enzyme immunoglobulin, microbial determination/testing, peptides, etc., can solve the problems of long-term stability of enzymes, decreased amplification ability, time-consuming and labor-consuming antibody screening, etc. , to achieve the effects of maintaining persistence, ensuring thermal stability, and protecting TaqDNA polymerase activity
CN111662386BActive Publication Date: 2021-03-30BEIJING TRANSGEN BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING TRANSGEN BIOTECH CO LTD
Publication Date
2021-03-30

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Abstract

The invention discloses a Taq DNA polymerase monoclonal antibody combination, a polymerase reaction system containing the same and application of the Taq DNA polymerase monoclonal antibody combination. The invention firstly discloses the Taq DNA polymerase monoclonal antibody combination, and the combination comprises a monoclonal antibody 4A8 and a monoclonal antibody 5H6. The invention further discloses application of the antibody combination in reducing non-specific amplification of Taq DNA polymerase and / or ensuring the thermal stability of the Taq DNA polymerase, the reaction system containing the combination, and a kit. The antibody combination and an optimized reaction solution can fully seal an active region of the Taq DNA polymerase under the condition of low temperature, effectively reduce non-specific amplification and ensure the thermal stability of the enzyme, and can still effectively maintain the durability of the enzyme activity under the condition of higher cycle number of amplification.
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Description

technical field

[0001] The present invention relates to the field of biotechnology. More specifically, it relates to a Taq DNA polymerase monoclonal antibody combination, a polymerase reaction system containing it, and applications thereof. Background technique

[0002] Polymerase chain reaction (polymerase chain reaction, PCR) technology is widely used in molecular biology experiments, a DNA molecule can be amplified 10 times in a few hours in vitro 9 times. PCR technology is similar to the natural replication process of double-stranded DNA. Under the catalysis of DNA polymerase, the mother-strand DNA is used as the template, and the specific primer is used as the starting point of extension. Through steps such as denaturation, annealing, and extension, the DNA of the parent-strand template is replicated in vitro. Complementary daughter strand DNA process. DNA polymerase is the key to DNA replication.

[0003] Taq DNA polymerase is a thermostable DNA polymerase isolated...

Claims

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