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A culture medium for human amniotic mesenchymal stem cells

A technology of high-quality stem cells and medium, applied in the field of stem cell culture, can solve the problems of reducing the number of cells, cytotoxicity, and high cost, and achieve the effect of increasing the number of cells cultured, enhancing the proliferation ability, and reducing the cost of preparation

Active Publication Date: 2021-07-13
爱存细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current choice for cell culture is serum medium. Although serum contains rich and comprehensive nutrients necessary for cell growth, it can provide hormones, growth factors and some low molecular weight nutrients to maintain exponential growth of cells. However, this medium The serum in the blood sometimes contains cytostatic factors and toxic factors, which are potentially toxic, and the cost of serum acquisition is high and the price is relatively expensive
[0005] Based on this, Chinese patent CN101914490B discloses a serum-free medium for human amniotic mesenchymal stem cells and a culture method thereof. The medium does not contain animal serum, and human serum albumin, human transferrin, Formed by human insulin and sodium selenite, although it is not subject to ethical restrictions and heterogeneous immunogenicity, a variety of proteins are added to this medium, which is easy to produce toxicity to cells and reduce the number of cells, and there are many types of proteins added. Increased the production cost of the culture medium is high
[0006] In summary, the medium in the prior art generally has the disadvantages of small number of cell cultures, high cost, and easy toxicity to cells

Method used

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  • A culture medium for human amniotic mesenchymal stem cells

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Effect test

Embodiment 1

[0029] Embodiment 1 A kind of human amniotic mesenchymal stem cell culture medium

[0030] The human amniotic mesenchymal stem cell medium includes advanced RPMI-1640 basal medium, cell growth factors, Ultroser G serum substitute, L-glutamine and honeysuckle powder; the advanced RPMI-1640 basal medium The addition amount is 20g / L, the addition amount of described cell growth factor is 1 μ g / L, the addition amount of described Ultroser G serum substitute is 40 mg / L, the addition amount of described L-glutamine is 3 μ g / L, The added amount of the honeysuckle powder is 2 μg / L; the cell growth factor is composed of PDGF, VEGF and EGF in a mass ratio of 1:2:9; the preparation process of the honeysuckle powder is as follows: take the dried honeysuckle, weigh Measure 1g, grind it with a grinder, and pass it through a 300-mesh sieve.

[0031] The preparation method of the human amniotic mesenchymal stem cell culture medium comprises the following steps:

[0032] S1. Take 400mL of pu...

Embodiment 2

[0035] Embodiment 2 A kind of culture medium for human amniotic mesenchymal stem cells

[0036] The human amniotic mesenchymal stem cell medium includes advanced RPMI-1640 basal medium, cell mitogens, Ultroser G serum substitute, L-glutamine and honeysuckle powder; the advanced RPMI-1640 basal medium The addition amount of said cell growth factor is 30g / L, the addition amount of said cell growth factor is 3μg / L, the addition amount of said Ultroser G serum substitute is 60mg / L, and the addition amount of said L-glutamine is 5μg / L , the addition of the honeysuckle powder is 4 μg / L; the cell growth factor is composed of PDGF, VEGF and EGF in a mass ratio of 3:5:11; the preparation process of the honeysuckle powder is: take the dried honeysuckle, Weigh 1g, grind it with a grinder, and pass it through a 300-mesh sieve.

[0037] The preparation method of the human amniotic mesenchymal stem cell culture medium comprises the following steps:

[0038] S1. Take 400mL of purified wate...

Embodiment 3

[0041] Embodiment 3 A kind of culture medium for human amniotic mesenchymal stem cells

[0042] The human amniotic mesenchymal stem cell medium includes advanced RPMI-1640 basal medium, cell mitogens, Ultroser G serum substitute, L-glutamine and honeysuckle powder; the advanced RPMI-1640 basal medium The addition amount of said cell growth factor is 25g / L, the addition amount of said cell growth factor is 2μg / L, the addition amount of said Ultroser G serum substitute is 50mg / L, and the addition amount of said L-glutamine is 4μg / L , the addition of the honeysuckle powder is 3 μg / L; the cell growth factor is composed of PDGF, VEGF and EGF in a mass ratio of 2:3:10; the preparation process of the honeysuckle powder is: take the dried honeysuckle, Weigh 1g, grind it with a grinder, and pass it through a 300-mesh sieve.

[0043] The preparation method of the human amniotic mesenchymal stem cell culture medium comprises the following steps:

[0044] S1. Take 400mL of purified wate...

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Abstract

The invention belongs to the field of stem cell culture, and in particular relates to a human amniotic mesenchymal stem cell culture medium. The human amniotic mesenchymal stem cell culture medium provided by the present invention includes advanced RPMI-1640 basal medium, cell growth factors, Ultroser G serum substitute, L-glutamine and honeysuckle powder. The culture medium for human amniotic mesenchymal stem cells provided by the present invention can promote cell division and cultivate more cells within a certain period of time without adding serum, thereby reducing the toxic effect of serum on cells and serum-derived pollution.

Description

technical field [0001] The invention belongs to the field of stem cell culture, and in particular relates to a human amniotic mesenchymal stem cell culture medium. Background technique [0002] Stem cells are cells of origin. Simply put, it is a type of primitive undifferentiated cell with multi-directional differentiation potential and self-replication ability, and is the original cell that forms various tissues and organs of mammals. Stem cells are common in shape, usually round or oval, small in size, relatively large in nucleus, mostly euchromatin in nucleus, and have high telomerase activity. [0003] The human amniotic membrane is located in the innermost part of the placenta, mainly composed of epithelial cells in the ectoderm and mesenchymal cells in the mesoderm. It does not contain blood vessels and has relatively simple cell components. It becomes waste after the fetus is delivered. Mesenchymal stem cells have many advantages, such as abundance, no need for inva...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/32C12N2500/76C12N2500/80C12N2501/11C12N2501/135C12N2501/165
Inventor 张印
Owner 爱存细胞生物科技有限公司