Kit based on nano plasma resonance molecules and test method

Abstract

The invention provides a kit based on nano plasma resonance molecules and a test method. The kit based on the nano plasma resonance molecules comprises a micro-pore plate, a nano optical sensor chip and a determination reagent, wherein the nano optical sensor chip is provided with a groove corresponding to a micro-pore array of the micro-pore plate, and the nano optical sensor chip is arranged onthe micro-pore plate in a manner that the groove is accommodated in the micro-pore; the determination reagent comprises a dilution reagent and a gold particle modified polyclonal antibody reagent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit and a test method based on nano-plasmon resonance molecules. Background technique [0002] Surface plasmon resonance (SPR) biosensors have become an important tool for studying the dynamics of biomolecular interactions due to the high sensitivity of the evanescent field generated by them to changes in the local refractive index (RI). The proposal of surface plasmon resonance (SPR) imaging combines SPR technology and imaging system closely, and becomes a technology with the characteristics of label-free, high-throughput, in situ and real-time detection of molecular interactions. It can be applied to measure specificity, affinity and kinetic parameters in the process of macromolecular binding, such as protein-protein, protein-DNA, receptor drug and cell / virus-protein binding. It has been widely used in early diagnosis of diseases, drug screening and food quality cont...

AI Technical Summary

Problems solved by technology

[0004] However, the existing detection equipment has problems such as complex operation, poor stability, slow detection speed, low sensitivity and expensive equipment, which is not conducive to the popularization of detection methods

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