Rhizoma bletillae small molecule characteristic fingerprint spectrum analysis method
A characteristic fingerprint and spectrum technology, applied in the field of pharmaceutical analysis, can solve problems such as different liquid phases and no standard products
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0134] Embodiment 1: white and test sample solution preparation
[0135] (1) Selection of different extraction solvents
[0136] ①Preparation of test sample solution: Accurately weigh three parts of Baiji sample, pulverize, pass through a No. 4 sieve to obtain 1.00g of Baiji powder, put it in a 100mL conical flask with a stopper, add pure water, 75% methanol, Ethanol 25mL, ultrasonic at room temperature for 30min, ultrasonic power 280W, frequency 40KHz, let cool, shake well, filter with 0.45μm microporous membrane to obtain the standard sample solution.
[0137] ②HPLC analysis conditions: C18 chromatographic column (250mm*4.6mm, 5μm); mobile phase adopts binary gradient elution system, phase A: 0.2% formic acid, phase B: acetonitrile; detection wavelength 280nm; column temperature 35℃ ; The elution gradient is shown in Table 1, the flow rate is 1.0 mL / min, and the injection volume is 10 μL.
[0138] Table 1 White and elution gradient
[0139]
[0140] ③Fingerprint establ...
Embodiment 2
[0169] Embodiment 2: white and chromatographic conditions are determined
[0170] (1) Selection of different column temperatures
[0171] ①Preparation of test sample solution: Accurately weigh the white powder, crush it, pass through a No. 4 sieve to obtain 1.00 g of white powder, put it in a 100 mL conical flask with a stopper, add 25 mL of 75% methanol, and ultrasonicate for 30 minutes at room temperature. Ultrasonic power 280W, frequency 40KHz, let cool, shake well, filter with 0.45μm microporous membrane to obtain standard sample solution.
[0172] ② HPLC analysis conditions: compare the influence of different column temperatures of 25° C., 35° C., and 45° C. on the separation performance, and other conditions are the same as in Example 1 (1) and ②.
[0173] ③Fingerprint establishment and result analysis: The higher the temperature, the better the chromatographic peaks of Baiji's fingerprint, but considering that higher column temperature will affect the life of the colum...
Embodiment 3
[0196] Embodiment 3: Determination of white and fingerprint spectrum methodology
[0197] (1) Determination of precision of white and fingerprint spectrum
[0198] ①Preparation of test sample solution: Accurately weigh the white powder, crush it, pass through a No. 4 sieve to obtain 1.00 g of white powder, put it in a 100 mL conical flask with a stopper, add 25 mL of 75% methanol, and ultrasonicate for 30 minutes at room temperature. Ultrasonic power 280W, frequency 40KHz, let cool, shake well, filter with 0.45μm microporous membrane to obtain standard sample solution.
[0199] ②HPLC analysis conditions: Agilent 1200LC HPLC; Stamsil-BpC18 column (250mm*4.6mm, 5μm); mobile phase adopts binary gradient elution system, phase A: 0.2% formic acid water, phase B : Acetonitrile; detection wavelength 280nm; column temperature 35°C; elution gradient as shown in Table 1, flow rate 1.0mL / min, injection volume 10μL.
[0200] 3. Get the same batch of Baiji sample, after preparing it acco...
PUM
Property | Measurement | Unit |
---|---|---|
wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com