Microbial limit inspection method for coptis chinensis formula granules

Abstract

The invention discloses a microbial limit inspection method for coptis chinensis formula granules. The microbial limit inspection method comprises the following steps: S1, respective preparation of bacterial suspensions of staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and aspergillus niger; S2, preparation of a test solution: preparing the test solution containing 3% histidine; S3, applicability test on a counting method for the total number of aerobic bacteria; S4, applicability test on a counting method for the total number of molds and saccharomycetes; and S5, applicability test on an Escherichia coli inspection method, wherein the limit detection standard is as follows: in 1 g of a test sample, the total number of aerobic bacteria is less than or equal to 103 cfu, the total number of molds and saccharomycetes is less than or equal to 102 cfu, and Escherichia coli cannot be detected. The method can be used for limit inspection of microorganisms in the coptis chinensis formula granules, and has the advantages of effectively eliminating the antibacterial activity of a sample, simplifying the inspection process, reducing the reagent dosage and efficiently detecting the microorganisms in the coptis chinensis formula granules.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, more specifically, it relates to a microbial limit inspection method for Coptis rhizome formula granules. Background technique [0002] Coptis chinensis has the effect of clearing away heat and dampness, purging fire and detoxifying. It is often used to treat damp-heat fullness, vomiting and acid regurgitation, diarrhea, jaundice, high fever and coma, hyperactivity of heart fire, upset and insomnia, vomiting of blood heat at the nose, red eyes, toothache , diabetes, carbuncle and furuncle and other symptoms, so Coptis chinensis is made into traditional Chinese medicine preparations for the treatment of related diseases. [0003] Coptidis is often made into granules. Before entering the market, according to the relevant requirements of the "Pharmacopoeia of the People's Republic of China" announced by the State Food and Drug Administration, the microbiological limit ...

AI Technical Summary

Problems solved by technology

[0005] The above is a conventional method for checking microbial limits in medicines, but the method for checking microbial limits in Coptis Rhizoma has not been disclosed yet

However, according to the microbial limit inspection method in traditional Chinese medicine recorded in the Pharmacopoeia of the People's Republic of China, it is necessary to add an effective neutralizer during the detection process. Bacteria, so as to effectively detect the limit of microorganisms in Coptis chinensis, but the "Pharmacopoeia of the People's Republic of China" does not record the neutralizer or inactivation method that can eliminate interfering substances in Coptis chinensis, and interfering substances will directly affect the microorganisms in Coptis chinensis Limit Check Results

Therefore, according to the relevant records of the Pharmacopoeia of the People's Republic of China, the microbial limit in Coptidis Rhizoma cannot be accurately detected

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