Unlock instant, AI-driven research and patent intelligence for your innovation.

Ensifer sesbaniae and application thereof in bioelectricity generation

A sword fungus and biological technology, applied in the field of environmental microorganisms and bioenergy, can solve the problems that have not been reported in the research, and achieve the effect of broad application prospects and strong electricity production activity

Active Publication Date: 2020-10-02
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently for Ensifer There are also many studies on the degradation of environmental pollutants, but no research reports on its use in the field of bioelectricity have been seen.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ensifer sesbaniae and application thereof in bioelectricity generation
  • Ensifer sesbaniae and application thereof in bioelectricity generation
  • Ensifer sesbaniae and application thereof in bioelectricity generation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Sword fungus ( Ensifer sesbaniae ) Screening isolation of Y5:

[0030] (1) Prepare the inoculum: River sediments were collected from black and odorous water bodies in Wenta Park, Ronggui, Foshan, Guangdong, and stored in a cold room (5-8°C). Take an appropriate amount of sediment samples to sieve (80 mesh), stir well and set aside (uniformly mixed sediment).

[0031] (2) The structure of the sediment microbial fuel cell device and the current collection system: the volume of the sediment microbial fuel cell is 1 L, and it is a single-chamber microbial fuel cell without a proton exchange membrane. Both cathode and anode are made of carbon felt (5×5 cm), and the electrodes are connected by titanium wire. The anode was connected to the titanium wire and placed at the bottom of the battery, 600 mL of uniformly mixed sediment was added, and then 300 mL of tap water was slowly added as the overlying water. After the cathode is connected to the titanium wire, it floats in ...

Embodiment 2

[0039] This embodiment discloses sword bacteria ( Ensifer sesbaniae ) Extracellular electron transport properties of Y5. As described in Example 1, sword bacteria ( Ensifer sesbaniae ) Y5 was inoculated into the anode compartment of the dual-compartment microbial fuel cell, and the cell was run until the voltage peaked and remained stable. Then, cyclic voltammetry scanning analysis was performed on the anode chamber of the battery. The potentiostat used CHI 700E, the Ag / AgCl electrode was used as the reference electrode, and the cathode was used as the counter electrode. Scanning voltage -0.6-0.2V, scanning speed is 10 mV / s, the results are as follows image 3 shown.

[0040] According to the existing literature reports, there are three main modes of electron transfer in electrogenic microorganisms: the extracellular transfer pathway of cytochrome on the outer membrane, the extracellular electron transfer pathway of nanowires on the outer membrane of the cell, and the ele...

Embodiment 3

[0042] This embodiment discloses sword bacteria ( Ensifer sesbaniae ) Y5 utilizes the electricity production characteristics of different carbon sources. The test method followed step 5 described in Example 1. The experimental results showed that the sword bacteria ( Ensifer sesbaniae ) Y5 can use carbon sources such as sodium formate, sodium acetate, sodium lactate and glucose to generate electricity, but the effects of electricity generation are not the same. Among them, when sodium acetate is used for electricity generation, the effect of electricity generation is the best ( Figure 4 ). What this embodiment discloses is sword fungus ( Ensifer sesbaniae ) The electricity production characteristics of Y5 in a dual-chamber microbial fuel cell, aiming to reveal that the bacteria can use a variety of carbon sources for electricity production. The dual-chamber microbial fuel cell and process used are not optimized. There have been many reports in the literature that the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Ensifer sesbaniae and an application thereof in bioelectricity generation. The Ensifer sesbaniae Y5 is preserved in the Guangdong Microbiological Culture Collection Center (GDMCC), wherein the preservation number is GDMCC No: 60957, and the preservation date is January 14, 2020. The Ensifer sesbaniae Y5 disclosed by the invention is screened and separated from black and odorous water body sediments, and the strain has relatively strong electricity generation capability, can grow in a relatively wide pH (5.5-9.5) and temperature (4-45 DEG C) range, can utilize multiple carbon sources to generate electricity, and a bright application prospect in the fields of environmental governance and energy production is achieved.

Description

technical field [0001] The invention relates to the technical fields of environmental microbes and bioenergy, in particular to a sword fungus with electricity production ability derived from black and odorous water sediments and its application in bioelectricity production. Background technique [0002] Electrogenic microorganisms (also known as extracellular electrogenic bacteria, anode respiration bacteria) refer to a type of microorganisms with extracellular electron transfer function that can transfer electrons generated by metabolism to extracellular electron acceptors, mostly anaerobic microorganisms or facultative anaerobic microorganisms. Electrogenic microorganisms are widely distributed in anaerobic or anoxic environments such as soil, wastewater, lake, ocean and river sediments. [0003] Microbial fuel cell is a bioelectrochemical reaction device that has developed rapidly in recent years. It integrates traditional biodegradation and electrochemical technology, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCC12N1/20H01M8/16C12R2001/01C12N1/205Y02E60/50Y02P70/50
Inventor 许玫英宋建华杨永刚
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY