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Bacillus PL-2 and application thereof to aquiculture

A technology of PL-2 and Bacillus, applied in the field of microorganisms, can solve problems such as imbalance of water quality factors, pollution of aquaculture water, secondary pollution, etc., and achieve the effects of improving growth conditions, increasing survival rates, and reducing control costs

Active Publication Date: 2020-10-02
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current aquaculture models are traditional aquaculture models that use multiple varieties, high density, and large amounts of fertilization and feeding to obtain high yields. Factors such as human activities, secretions and excrements of the aquaculture objects themselves, excess bait, and drug residues are extremely important. It is easy to lead to the pollution of aquaculture water and the imbalance of water quality factors, so that the content of ammonia nitrogen, phosphorus, nitrite, hydrogen sulfide and other substances in the water will increase rapidly, which will cause disease and even mass death of aquaculture animals
[0003] At present, feed drug additives mainly based on antibiotics, hormones, and preservatives have brought great benefits to aquaculture production. Feed containing these feed drug additives can achieve the purpose of controlling the occurrence of some diseases and promoting the growth of farmed animals. As a result, problems such as drug residues, secondary pollution, and aquatic product quality and safety have become more and more serious.

Method used

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  • Bacillus PL-2 and application thereof to aquiculture
  • Bacillus PL-2 and application thereof to aquiculture
  • Bacillus PL-2 and application thereof to aquiculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The method utilizing bacillus PL-2 to prepare microbial inoculum specifically comprises the following steps:

[0075] (1) Streak-inoculate Bacillus PL-2 on the solid medium of beef extract peptone, and culture it upside down at 30°C for 24 hours to obtain a single colony. The formula of the solid medium of beef extract peptone is: 3.0g of beef extract, peptone 10.0g, NaCl5.0g, agar 15g, water 1000ml;

[0076] (2) Pick a single colony from beef extract peptone solid medium and inoculate it into LB liquid medium, activate and cultivate at 35°C and 150rpm for 10 hours to obtain seed solution;

[0077] (3) Inoculate the obtained seed liquid into the fermentation medium at an inoculation ratio of 3%, and amplify the culture at 30°C and 200rpm for 20 hours to obtain a fermentation liquid. The formula of the fermentation medium is: NH 4 Cl 1g, CH 3 COONa 3g, MgCl 2 0.2g, CaCl 2 0.2g, KH 2 PO 4 0.6g, NaCl 1g, yeast extract 2g, glucose 2g, water 1000ml;

[0078] (4) Centr...

Embodiment 2

[0081] The method utilizing bacillus PL-2 to prepare microbial inoculum specifically comprises the following steps:

[0082] (1) Streak-inoculate Bacillus PL-2 on the solid medium of beef extract peptone, and culture it upside down at 30°C for 24 hours to obtain a single colony. The formula of the solid medium of beef extract peptone is: 3.0g of beef extract, peptone 10.0g, NaCl5.0g, agar 15g, water 1000ml;

[0083] (2) Pick a single colony from beef extract peptone solid medium and inoculate it into LB liquid medium, activate and cultivate at 35°C and 150rpm for 10 hours to obtain seed solution;

[0084] (3) Inoculate the obtained seed liquid into the fermentation medium at an inoculation ratio of 3%, and amplify the culture at 30°C and 200rpm for 20 hours to obtain a fermentation liquid. The formula of the fermentation medium is: NH 4 Cl 1g, CH 3 COONa 3g, MgCl 2 0.2g, CaCl 2 0.2g, KH 2 PO 4 0.6g, NaCl 1g, yeast extract 2g, glucose 2g, water 1000ml;

[0085] (4) Centr...

Embodiment 3

[0088] The method utilizing bacillus PL-2 to prepare microbial inoculum specifically comprises the following steps:

[0089] (1) Streak-inoculate Bacillus PL-2 on the solid medium of beef extract peptone, and culture it upside down at 30°C for 24 hours to obtain a single colony. The formula of the solid medium of beef extract peptone is: 3.0g of beef extract, peptone 10.0g, NaCl5.0g, agar 15g, water 1000ml;

[0090] (2) Pick a single colony from beef extract peptone solid medium and inoculate it into LB liquid medium, activate and cultivate at 35°C and 150rpm for 10 hours to obtain seed solution;

[0091] (3) Inoculate the obtained seed solution into the fermentation medium at an inoculation ratio of 5%, and amplify the culture at 30°C and 200rpm for 20 hours to obtain a fermentation solution. The formula of the fermentation medium is: NH 4 Cl 1g, CH 3 COONa 3g, MgCl 2 0.2g, CaCl 2 0.2g, KH 2 PO 4 0.6g, NaCl 1g, yeast extract 2g, glucose 2g, water 1000ml;

[0092] (4) C...

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Abstract

The invention discloses bacillus PL-2 and an application thereof to aquiculture. The preservation No. of the bacillus PL-2 is CCTCC NO:M 2020049, and the bacillus PL-2 is separated and purified from deposit samples from a breeding pond in South China Sea New District of Weihai City, and the bacillus PL-2 has favorable degradation effects on ammonia nitrogen, hydrogen sulphide and organophosphor. Fermentation products have favorable inhibition effects on vibrio alginolyticus and aeromonas hydrophila, can be applied to a breeding water area in a microorganism microbial inoculum manner, are a biological control means, and have the characteristics of being environmental-friendly and pollution-free compared with chemical control, and being lower in control cost and higher in benefits compared with physical control.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a bacillus PL-2 and its application in aquaculture, and belongs to the technical field of microorganisms. Background technique [0002] Water is the environment that aquatic animals must rely on for their survival and growth. The quality of water is the key to the success of aquaculture, especially intensive farming. Most of the current aquaculture models are traditional aquaculture models that use multiple varieties, high density, and large amounts of fertilization and feeding to obtain high yields. Factors such as human activities, secretions and excrements of the aquaculture objects themselves, excess bait, and drug residues are extremely important. It is easy to lead to the pollution of aquaculture water and the imbalance of water quality factors, so that the content of ammonia nitrogen, phosphorus, nitrite, hydrogen sulfide and other substances in the water will incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/20
CPCC02F3/34C02F2103/20C12N1/20C12N1/205C12R2001/07
Inventor 彭健方素云胡晓珂闵军
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI