A method for improving the tolerance of probiotics to ciprofloxacin

A technology of ciprofloxacin and probiotics, which is applied in the biological field, can solve the problems of antibiotic abuse and achieve the effects of rapid coating, good biocompatibility, and good defense and protection

Active Publication Date: 2022-03-18
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, overuse of antibiotics can lead to serious problems

Method used

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  • A method for improving the tolerance of probiotics to ciprofloxacin
  • A method for improving the tolerance of probiotics to ciprofloxacin
  • A method for improving the tolerance of probiotics to ciprofloxacin

Examples

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Embodiment 1

[0044] A method for improving the tolerance of probiotics to ciprofloxacin, specifically carried out according to the following steps:

[0045] 1) Preparation of carboxymethyl chitosan: Disperse 50 mg of chitosan in 5 mL of isopropanol to obtain a chitosan dispersion (insoluble); use 1 mL of deionized water to dissolve 100 mg of sodium hydroxide, add chitosan dispersion Afterwards, alkalized for 30 min; dissolved 100 mg of chloroacetic acid in 1 mL of isopropanol, then added dropwise into the alkalized chitosan solution at a rate of 50 μL / min, reacted at 30 °C for 3 h, and terminated by adding 10% hydrochloric acid. Reaction, cleaning, vacuum drying, and finally carboxymethyl chitosan;

[0046] 2) Preparation of carboxydextran: Dissolve 100 mg of dextran in 10 mL of dimethyl sulfoxide to prepare a dextran solution, then add 300 mg of succinic anhydride and 5 mg of catalyst 4-dimethylaminopyridine to the dextran solution, Stir magnetically in an oil bath at 40°C for 10 hours, ...

Embodiment 2

[0050] The difference between this example and Example 1 is: the ratio of chitosan mass and isopropanol volume in step 1) is 100 mg: 10 mL; the ratio of sodium hydroxide to chitosan mass concentration is 1:6 ; The ratio of the mass of chloroacetic acid to the volume of isopropanol is 150mg: 10mL; others are the same as in Example 1.

Embodiment 3

[0052] The difference between this example and Example 1 or 2 is that: the volume ratio of the mass of dextran described in step 2) to dimethyl sulfoxide is 200 mg:15 mL; the mass ratio of the mass of succinic anhydride to dimethyl sulfoxide The volume ratio of sulfone is 380mg:15mL; the mass ratio of the dextran to 4-dimethylaminopyridine is 1:0.05; the others are the same as those in Example 1 or 2.

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Abstract

The invention discloses a method for improving the tolerance of probiotics to ciprofloxacin. By constructing an artificial cell wall of two-component polysaccharides based on the surface of probiotics, the protective coating and controllable detachment of yeast cells are carried out. Go, improve the ability of probiotics such as Saccharomyces cerevisiae to tolerate excessive antibiotics. The present invention takes Saccharomyces cerevisiae probiotics as basic microorganisms, prepares carboxymethyl chitosan (CMCS) and carboxy dextran (DEX-COOH), and coats them on the cell surface to form a two-component polysaccharide artificial cell wall. The specific degradation effect of chitosanase is used to achieve the degradation of the constructed artificial cell wall and realize its "wearing-unloading" process. This method allows the microorganisms to be fully protected, not only maintaining the high activity of Saccharomyces cerevisiae probiotics, but also improving their ability to tolerate excessive antibiotics.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the tolerance of probiotics to ciprofloxacin. 【Background technique】 [0002] In recent years, antibiotics, as irreplaceable functional feed additives in aquaculture, are widely used in aquaculture to alleviate infectious diseases, improve growth performance and enhance immunity. The over-reliance on antibiotics in the aquaculture industry has led to the increasingly serious problem of antibiotic abuse, and further endangered human health and water environment pollution. The aquaculture industry is facing huge challenges, and there is an urgent need to develop natural functional feed additives such as probiotics. As a beneficial active microorganism, probiotics can endow the host with various beneficial effects such as inhibiting bacterial diseases, improving the body's immune response, and promoting water material circulation, and are favored in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/865
CPCC12N1/04
Inventor 尹艳镇胡汉娇梁兴唐张霞汪双双郑韵英张瑞瑞
Owner BEIBU GULF UNIV
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