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Method for improving verticillium dahliae resistance of plant by utilizing AtALA7 gene

A technology for Verticillium dahliae and transgenic plants is applied in the field of improving plant resistance to Verticillium dahliae, and can solve problems such as difficulty in achieving broad-spectrum effects, verticillium wilt damage and the like

Active Publication Date: 2020-10-09
SOUTHWEST UNIVERSITY
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Problems solved by technology

Traditional control techniques and the cultivation of new varieties resistant to Verticillium wilt have effects in one place and one place in the prevention and control of Verticillium wilt, and Verticillium wilt rapidly differentiates. It is difficult to achieve broad-spectrum effects through conventional control measures. Verticillium wilt The threat of wilt is still serious

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  • Method for improving verticillium dahliae resistance of plant by utilizing AtALA7 gene
  • Method for improving verticillium dahliae resistance of plant by utilizing AtALA7 gene
  • Method for improving verticillium dahliae resistance of plant by utilizing AtALA7 gene

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Embodiment Construction

[0073] The present invention will be described in further detail below in conjunction with the accompanying drawings, but the following description does not limit the present invention.

[0074] The pharmaceutical reagents in the implementation examples of the present invention are domestic conventional chemical reagents that are not specified, and all materials and methods that are not specified are referred to "Molecular Cloning Experiment Guide" (Sambrook and Russell, 2001).

[0075] 1. Proposal of the core strategy of the invention technology

[0076] 1.1 Obtaining homozygous mutants of Arabidopsis thaliana with deletion of AtALA7 gene

[0077] 1.1.1 Arabidopsis mutant acquisition and planting

[0078] Arabidopsis mutants were purchased from the Arabidopsis Biological Resource Center (ABRC) T-DNA insertion mutants ( Figure 4 A-C), number: ala7(SALK_125598).

[0079] Arabidopsis seed culture method: divide the seeds into 1.5mL centrifuge tubes, oscillate repeatedly with...

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Abstract

The invention relates to a method for improving verticillium dahliae resistance of plants. An AtALA7 gene is integrated into a target plant to obtain a transgenic plant, and the AtALA7 gene is expressed in the plant, so that the resistance of the plant to verticillium dahliae is improved, and the nucleotide sequence of the AtALA7 gene is as shown in SEQ ID NO.12. According to the invention, the resistance of arabidopsis thaliana, tobacco and cotton to verticillium wilt bacteria is improved by utilizing a transport degradation mechanism of plants to pathogenic toxins. Therefore, the invention can be widely applied to improving the resistance of different pathogenic bacteria and toxins thereof, and has wide applicability.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, it relates to a method for increasing plant resistance to Verticillium dahliae. Background technique [0002] Verticillium wilt is a widespread worldwide disease, spreading across dozens of countries such as the United States, China, India, and Brazil (Watkins, 1981; Bell, 1992). Verticillium dahliae has a wide range of hosts, as many as 660 species of plants, involving Solanaceae, Rosaceae, Fabaceae, Cruciferae, etc., including 184 species of crops, 323 species of ornamental plants, and 153 species of weeds (Chen Xusheng, 1998); Crops such as potato, eggplant, tomato, pepper, watermelon, peanut, tobacco (Bhat and Subbarao, 1999), woody plants such as zinc tree, olive and other plants can be infected by Verticillium wilt (Mercado et al., 2002), And can transfect each other and aggravate the damage, the global annual loss caused by Verticillium wilt reaches billions of doll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/55C12N15/65A01H5/00A01H6/20A01H6/82A01H6/60
CPCC12N9/14C12N15/8282C12N15/65C12Y306/03
Inventor 裴炎王凡龙侯磊李先碧苏梅卓静欣李玉杰陈杨宋水清
Owner SOUTHWEST UNIVERSITY
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