Method for improving verticillium dahliae resistance of plant by utilizing AtALA7 gene
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for Verticillium dahliae and transgenic plants is applied in the field of improving plant resistance to Verticillium dahliae, and can solve problems such as difficulty in achieving broad-spectrum effects, verticillium wilt damage and the like
Active Publication Date: 2020-10-09
SOUTHWEST UNIVERSITY
View PDF5 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Traditional control techniques and the cultivation of new varieties resistant to Verticillium wilt have effects in one place and one place in the prevention and control of Verticillium wilt, and Verticillium wilt rapidly differentiates. It is difficult to achieve broad-spectrum effects through conventional control measures. Verticillium wilt The threat of wilt is still serious
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0073] The present invention will be described in further detail below in conjunction with the accompanying drawings, but the following description does not limit the present invention.
[0074] The pharmaceutical reagents in the implementation examples of the present invention are domestic conventional chemical reagents that are not specified, and all materials and methods that are not specified are referred to "Molecular Cloning Experiment Guide" (Sambrook and Russell, 2001).
[0075] 1. Proposal of the core strategy of the invention technology
[0076] 1.1 Obtaining homozygous mutants of Arabidopsis thaliana with deletion of AtALA7 gene
[0077] 1.1.1 Arabidopsis mutant acquisition and planting
[0078] Arabidopsis mutants were purchased from the Arabidopsis Biological Resource Center (ABRC) T-DNA insertion mutants ( Figure 4 A-C), number: ala7(SALK_125598).
[0079] Arabidopsis seed culture method: divide the seeds into 1.5mL centrifuge tubes, oscillate repeatedly with...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a method for improving verticillium dahliae resistance of plants. An AtALA7 gene is integrated into a target plant to obtain a transgenic plant, and the AtALA7 gene is expressed in the plant, so that the resistance of the plant to verticillium dahliae is improved, and the nucleotide sequence of the AtALA7 gene is as shown in SEQ ID NO.12. According to the invention, the resistance of arabidopsis thaliana, tobacco and cotton to verticillium wilt bacteria is improved by utilizing a transport degradation mechanism of plants to pathogenic toxins. Therefore, the invention can be widely applied to improving the resistance of different pathogenic bacteria and toxins thereof, and has wide applicability.
Description
technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, it relates to a method for increasing plant resistance to Verticillium dahliae. Background technique [0002] Verticillium wilt is a widespread worldwide disease, spreading across dozens of countries such as the United States, China, India, and Brazil (Watkins, 1981; Bell, 1992). Verticillium dahliae has a wide range of hosts, as many as 660 species of plants, involving Solanaceae, Rosaceae, Fabaceae, Cruciferae, etc., including 184 species of crops, 323 species of ornamental plants, and 153 species of weeds (Chen Xusheng, 1998); Crops such as potato, eggplant, tomato, pepper, watermelon, peanut, tobacco (Bhat and Subbarao, 1999), woody plants such as zinc tree, olive and other plants can be infected by Verticillium wilt (Mercado et al., 2002), And can transfect each other and aggravate the damage, the global annual loss caused by Verticillium wilt reaches billions of doll...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more