Urokinase adsorbent as well as preparation method and application thereof

A technology of urokinase and adsorbent, which is applied in the field of medical biology, can solve the problems of unsuitability for large-scale production, high cost, and low crude extraction yield of urokinase, and achieve low cost, low environmental protection pressure, and high crude extraction yield Effect

Active Publication Date: 2020-10-13
JIANGSU YOULIKA BIOTECHNOLOYG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above method solves a series of environmental problems caused by the collection and transportation of primary urine in the traditional method, it still has problems such as high cost, low crude extraction yield of urokinase, specific activity, etc., and is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 A kind of urokinase adsorbent and preparation method thereof

[0048]Dry the silica gel in an oven at 125°C for 4 hours, add 100g of activated silica gel into a 1L three-necked reaction flask, add 400ml of toluene, 50ml of vinylmethyldiethoxysilane, reflux for 8h, filter, extract with ethanol, wash with water, and obtain the former Body 1, add precursor 1 and 400ml water into a 1L three-necked flask, add bromine dropwise until the aqueous solution just turns red, react at room temperature (25°C) for 30 minutes, filter and wash with water to obtain precursor 2, mix precursor 2 and 400ml of water was added to a 1L three-neck flask, 20g of p-aminobenzamidine hydrochloride was added to adjust the pH to 9, and the temperature was raised to 80°C for 8 hours. Cool down to 30°C, filter, extract with ethanol, wash with water, and spin dry to obtain the urokinase adsorbent.

Embodiment 2

[0050] A kind of urokinase adsorbent and preparation method thereof

[0051] Dry the silica gel in an oven at 120°C for 10 hours, add 100g of activated silica gel into a 1L three-necked reaction flask, add 350ml of methanol, 45ml of vinyltriisopropoxysilane, reflux for 6h, filter, extract with ethanol, and wash with water to obtain the precursor 1. Put the precursor 1 and 350ml of water into a 1L three-necked flask, add bromine dropwise until the aqueous solution just turns red, react at room temperature for 30 minutes at 25°C, filter and wash to obtain the precursor 2, mix the precursor 2 and 350ml of water Add it into a 1L three-necked flask, add 10g of p-aminobenzamidine hydrochloride, adjust the pH to 9, react at 60°C for 10 hours, cool down to 30°C, filter, extract with ethanol, wash with water, and dry to obtain the urokinase adsorbent .

Embodiment 3

[0053] A kind of urokinase adsorbent and preparation method thereof

[0054] Dry the silica gel in an oven at 160°C for 3 hours, add 100g of activated silica gel into a 1L three-necked reaction flask, add 450ml of ethanol, 55ml of vinyltriacetoxysilane, reflux for 10h, filter, extract with ethanol, and wash with water to obtain the precursor 1, Add precursor 1 and 450ml of water into a 1L three-necked flask, add bromine dropwise until the aqueous solution just turns red, react at room temperature for 30 minutes at 25°C, filter and wash with water to obtain precursor 2, add precursor 2 and 450ml of water to Add 30g of p-aminobenzamidine hydrochloride to a 1L three-necked flask, adjust the pH to 10, react at 90°C for 4 hours, cool down to 30°C, filter, extract with ethanol, wash with water, and dry to obtain the urokinase adsorbent.

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PUM

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Abstract

The invention discloses a urokinase adsorbent as well as a preparation method and application thereof. The preparation method comprises the steps of S1, activating silica gel at high temperature to obtain activated silica gel; S2, reacting the activated silica gel obtained in the step S1 with a coupling agent in an organic solvent, filtering, extracting with ethanol, and washing with water to obtain a precursor 1; S3, reacting the precursor 1 obtained in the step S2 with bromine, filtering, and washing with water to obtain a precursor 2; and S4, adding water and p-aminobenzamidine hydrochloride into the precursor 2 obtained in the step S3, adjusting the pH value, reacting, cooling, filtering, extracting with ethanol, washing with water, and spin-drying to obtain the product. The urokinaseadsorbent prepared by the method and the method for directly adsorbing urokinase from human urine by using the urokinase adsorbent are provided. The urokinase adsorbent prepared in the invention has the advantages of strong specificity, wide application range, low cost, realization of direct adsorption of urokinase from human urine, high crude extraction yield and high specific activity of urokinase, and realization of large-scale production of urokinase crude products.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and in particular relates to an adsorbent capable of directly adsorbing urokinase from human urine, a preparation method and application thereof. Background technique [0002] Urokinase is a serine protease produced by human renal tubular epithelial cells. It is a basic protein with an isoelectric point around pH 8.7. It is white non-crystalline powder, easily soluble in water. Dilute solution is unstable and must be used fresh, and must not be diluted with acidic solution. The freeze-dried state is stable for several years. Urokinase is a highly specific proteolytic enzyme. The activity on synthetic substrates is similar to that of trypsin and plasmin, and it also has the activity of esterase. Non-antigenic, does not cause antibodies in the body. The half-life in vivo is (14±6)min. [0003] Urokinase is a thrombolytic drug, which can activate plasminogen into active plasmin, and plasmi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/30C12N9/72
CPCB01J20/103B01J20/22C12N9/6462C12Y304/21031Y02A50/30
Inventor 顾京李争刘艳红丁晶黄俊杰熊心磊
Owner JIANGSU YOULIKA BIOTECHNOLOYG CO LTD
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