Low-temperature preservation process for Taxus chinensis var

Abstract

The invention discloses a low-temperature preservation process of Taxus chinensis var. The low-temperature preservation process includes the basic steps that a superior tree is selected for collectingseeds, arils of the collected seeds are washed, then the seeds are exposed to the sun for 2-3 days, stored in a bag for storage, the temperature is 4-5 DEG C, the seeds are place for 5-7 months, after natural temperature recovery, the dormancy is broken, after mechanically removing of hulls is carried out, soaking is carried out in distilled water for 24 hours, changing of water is carried out for several times until the inner coats of the seeds are removed, then the processed seeds are placed under sterile conditions and placed in 1% potassium permanganate solution for soaking and disinfection for 30 minutes, then rinsing with sterile water is carried out, the processed seeds are transferred to a freeze pipe, after 10% DMSO cryoprotectant is added, the freeze pipe is sealed and then placed in an ice bath, soaking treatment is carried out under 250W ultrasonic conditions for 10-15 minutes, then the processed pipe is put in a cold storage at -16-(-18) DEG C to pre-froze for 75min, andthen the processed pipe is put in liquid nitrogen for freezer storage after treatment is completed. Compared with traditional seed preservation methods, the process can further enhance seed vigor, andgreatly increase the amount of TTC reduction, the preservation time is longer, the cost is lower, and the economic and social benefits are better.

Description

technical field [0001] The invention belongs to the field of yew cultivation and propagation, and in particular relates to a low-temperature preservation process of southern yew seeds. Background technique [0002] Taxus, a plant of the genus Cedar, is recognized as an endangered natural and rare anti-cancer plant in the world. It is also a first-class national protected plant in my country. There are only 11 species in the world, mainly distributed in East Asia, North America and China. There are 4 species in my country. and 1 variant, both have been confirmed to contain the new anticancer drug paclitaxel and its analogues, so the medicinal, biological and economic value is great, but in the past due to its unique material advantages, the wild yew was deforestation The population has suffered a severe blow. In addition, under natural conditions, the growth rate of yew is slow and the regeneration ability is poor, which makes large-scale natural raw material forest bases even ...

AI Technical Summary

Problems solved by technology

[0002] Taxus, a plant of the genus Cedar, is recognized as an endangered natural and rare anti-cancer plant in the world. It is also a first-class national protected plant in my country. There are only 11 species in the world, mainly distributed in East Asia, North America and China. There are 4 species in my country. and 1 variant, both have been confirmed to contain the new anticancer drug paclitaxel and its analogues, so the medicinal, biological and economic value is great, but in the past due to its unique material advantages, the wild yew was deforestation The population has suffered a severe blow. In addition, under natural conditions, the growth rate of yew is slow and the regeneration ability is poor, which makes large-scale natural raw material forest bases even rarer. Moreover, the dormancy period of yew seeds is long, and the germination rate and emergence rate are low. Difficulty in afforestation and introduction