Bio-friendly antibacterial coating capable of effectively fixing cationic antibacterial agent and resisting bacterial biofilm

An antibacterial coating and cation technology, applied in the field of medical materials, can solve the problems of biofilm pollution and poor biocompatibility, and achieve the effects of simple preparation method, poor biocompatibility improvement, and long-term high-efficiency effects.

Active Publication Date: 2020-10-16
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings that the surface based on cationic antibacterial agent is easily polluted by biofilm and poor biocompatibility, and provide a kind of antibacterial agent that is easy to prepare, biologically safe, does not cause bacterial drug resistance, and can stably adhere to various medical antimicrobial agents. Multifunctional coatings on material surfaces

Method used

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  • Bio-friendly antibacterial coating capable of effectively fixing cationic antibacterial agent and resisting bacterial biofilm
  • Bio-friendly antibacterial coating capable of effectively fixing cationic antibacterial agent and resisting bacterial biofilm
  • Bio-friendly antibacterial coating capable of effectively fixing cationic antibacterial agent and resisting bacterial biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] After mixing 10mL of 10mg / mL bovine serum albumin aqueous solution and 10mL of 0.4g / mL polylysine aqueous solution evenly, add 10mL of 14mg / mL tris (2-carboxyethyl) phosphine aqueous solution with pH of 4.5 (its pH is determined by NaOH adjustment), mixed uniformly to obtain an impregnating liquid, the concentration of bovine serum albumin in the obtained impregnating liquid was 3.33mg / mL, the concentration of polylysine was 0.13g / mL, and the concentration of three (2-carboxyethyl) phosphine It was 4.67mg / mL. Soak a silica gel sheet with a length and width of 1.8 cm in the obtained immersion solution at 30°C for 2 hours, then wash it with deionized water and dry it to obtain a silica gel modified with a phase-transition bovine serum albumin antibacterial coating immobilized with polylysine piece.

Embodiment 2

[0030]After mixing 10mL of 10mg / mL bovine serum albumin aqueous solution and 10mL of 0.4g / mL polylysine aqueous solution evenly, add 10mL of 14mg / mL tris (2-carboxyethyl) phosphine aqueous solution with pH of 4.5 (its pH is determined by NaOH adjustment), mixed uniformly to obtain an impregnating liquid, the concentration of bovine serum albumin in the obtained impregnating liquid was 3.33mg / mL, the concentration of polylysine was 0.13g / mL, and the concentration of three (2-carboxyethyl) phosphine It was 4.67mg / mL. Soak a silica gel sheet with a length and a width of 1.8 cm in the obtained immersion solution at 30°C for 2 hours, then wash it with deionized water, dry it, and soak it in the above immersion solution again, repeating 4 times to obtain a polylysine-immobilized Phase-transition bovine serum albumin antimicrobial coating modified silica gel sheets.

Embodiment 3

[0034] After mixing 10mL of 15mg / mL bovine serum albumin aqueous solution and 10mL of 0.4g / mL polylysine aqueous solution evenly, add 10mL of 10mg / mL aqueous solution of β-mercaptoethanol with a pH of 5.0 (the pH of which is adjusted by NaOH), and mix Uniformly, obtain soaking liquid, the concentration of bovine serum albumin in the gained soaking liquid is 3mg / mL, the concentration of polylysine is 0.13g / mL, the concentration of β-mercaptoethanol is 3.33mg / mL. Soak the polyurethane in the obtained impregnating solution at 30°C for 2 hours, then wash it with deionized water, dry it, soak it again in the above impregnating solution, and repeat 4 times to obtain a phase-transition bovine serum albumin antibacterial coating with polylysine fixed. Coated polyurethane.

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Abstract

The invention discloses a bio-friendly antibacterial coating capable of effectively fixing a cationic antibacterial agent and resisting a bacterial biofilm. The coating can be formed on the surface ofa to-be-modified substrate by placing the to-be-modified substrate in impregnation liquid, and the impregnation liquid is formed by mixing protein, the cationic antibacterial agent and a reducing agent; negative charges, which are presented in an environment with an isoelectric point larger than that of natural proteins such as serum albumin, fibrinogen, alpha-lactalbumin and the like by the natural proteins, can be mutually neutralized with positive charges of the cationic antibacterial agent, thereby obtaining the coating with a surface close to electric neutrality, protein and bacterial debris adsorption can be effectively avoided, the long-term efficient antibacterial and anti-biofilm effects are maintained, and meanwhile, the defect of poor biocompatibility of the bactericidal surface of a traditional cationic antibacterial agent is overcome. The coating disclosed by the invention is simple and convenient in preparation method, biologically safe and universally applicable to thesurfaces of various medical devices, the bactericidal effect of the coating is achieved without depending on antibiotics, and bacterial drug resistance caused by improper use of antibiotics can be effectively avoided.

Description

technical field [0001] The invention belongs to the technical field of medical materials, in particular to a bio-friendly antibacterial coating capable of fixing cationic antibacterial agents and resisting bacterial biofilms. Background technique [0002] With the development of a variety of medical implantable devices, implant-related infections will not only cause local tissue inflammation, but also shorten the service life of implants and even cause serious complications such as sepsis, causing a significant economic burden on patients and society . In recent years, although a variety of antibacterial coatings based on antibiotics or nanosilver have shown good bactericidal effects, their practical value has been limited due to the problem of bacterial resistance caused by these surfaces and the pollution caused to the environment. The long-term excessive and inappropriate use of antibiotics by humans has dramatically accelerated the occurrence of drug-resistant mutations...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/08A61L31/02A61L31/06A61L31/14A61L31/16
CPCA61L31/10A61L31/08A61L31/028A61L31/026A61L31/022A61L31/06A61L31/14A61L31/16A61L2300/404A61L2300/252A61L2300/254A61L2300/208A61L2300/206A61L2400/18A61L2420/02A61L2420/06C08L89/00C08L77/04C08L75/04C08L61/16
Inventor 杨鹏田娟华胡心怡
Owner SHAANXI NORMAL UNIV
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