Respiratory tract virus nucleic acid sampling test kit and application thereof

A virus nucleic acid and respiratory technology, applied in the field of biomedicine, can solve the hidden dangers of epidemic prevention and control, insufficient sample collection and other problems, and achieve the effect of reducing the discomfort of sampling, convenient sampling, and improving the detection rate of nucleic acids.

Active Publication Date: 2020-10-16
ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current nucleic acid detection rate of the new coronavirus is only 30%-50%, which means that 50-70% of the infected people have been missed. major hidden danger
Conventional nasopharyngeal swab and oropharyngeal swab sampling method, insufficient sample collection is the key factor causing missed detection

Method used

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  • Respiratory tract virus nucleic acid sampling test kit and application thereof
  • Respiratory tract virus nucleic acid sampling test kit and application thereof
  • Respiratory tract virus nucleic acid sampling test kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a sampling kit for respiratory virus nucleic acid detection, including nasal spray and sample preservation solution 1, the nasal spray comprises: 0.9% sodium chloride, 0.5% sodium pyruvate according to the composition of weight percentage , 0.01% pyruvate, 0.1% Tween 80, 0.01% glyceryl monostearate, and the balance is sterile water; the sample preservation solution-comprises according to weight percentage: 0.12% glucose, 0.02 % calcium chloride, 0.05% potassium chloride, 0.01% disodium hydrogen phosphate, 0.015% isoleucine, 0.01% leucine, 0.015% lysine hydrochloride, the balance is sterile physiological brine.

Embodiment 2

[0030] This embodiment provides a sampling kit for respiratory virus nucleic acid detection, including nasal spray and sample preservation solution 1, the nasal spray comprises: 3% sodium chloride, 0.1% sodium pyruvate according to the composition of weight percentage , 0.1% pyruvate, 0.01% Tween 80, 0.1% glyceryl monostearate, and the balance is sterile water; the sample preservation solution-comprises according to weight percentage: 0.08% glucose, 0.03 % calcium chloride, 0.03% potassium chloride, 0.015% disodium hydrogen phosphate, 0.01% isoleucine, 0.015% leucine, 0.01% lysine hydrochloride, the balance is sterile physiological brine.

Embodiment 3

[0032] This embodiment provides a sampling kit for respiratory virus nucleic acid detection, including nasal spray and sample preservation solution 1, the nasal spray comprises: 1.2% sodium chloride, 0.3% sodium pyruvate according to the composition of weight percentage , 0.05% pyruvate, 0.05% Tween 80, 0.05% glyceryl monostearate, and the balance is sterile water; the sample preservation solution-comprises according to weight percentage: 0.10% glucose, 0.03 % calcium chloride, 0.04% potassium chloride, 0.012% disodium hydrogen phosphate, 0.013% isoleucine, 0.012% leucine, 0.012% lysine hydrochloride, the balance is sterile physiological brine.

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Abstract

The invention provides a respiratory tract virus nucleic acid sampling test kit and application thereof, the test kit comprises a nasal spray and a sample preserving fluid, and the nasal spray comprises the following components by weight percentage: 0.9%-3% of sodium chloride, 0.1%-0.5% of sodium pyruvate, 0.01%-0.1% of pyruvic acid, 0.01%-0.1% of Tween 80, 0.01%-0.1% of glyceryl monostearate andthe balance of sterile water; the sample preserving fluid is prepared from the following components in percentage by weight: 0.08 to 0.12 percent of glucose, 0.02 to 0.03 percent of calcium chloride,0.03 to 0.05 percent of potassium chloride, 0.01 to 0.015 percent of disodium hydrogen phosphate, 0.01 to 0.015 percent of isoleucine, 0.01-0.015% of leucine, 0.01-0.015% of lysine hydrochloride and the balance normal saline. The method can effectively improve the nucleic acid detection rate of the respiratory tract virus.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a respiratory virus nucleic acid sampling kit and application thereof. Background technique [0002] Respiratory virus infection is the most likely way of causing large-scale public health incidents. The "COVID-19" epidemic continues to bring huge disasters to the world. According to the "Diagnosis and Treatment Plan for Novel Coronavirus Pneumonia" issued by the National Health Commission, viral nucleic acid detection is the main diagnostic criterion. [0003] However, the current nucleic acid detection rate of the new coronavirus is only 30%-50%, which means that 50-70% of the infected people have been missed. major hazards. Conventional nasopharyngeal swab and oropharyngeal swab sampling methods, insufficient sample collection, is the key factor causing missed detection. Contents of the invention [0004] In view of this, it is necessary to address the pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806C12Q1/70C12N15/10C12R1/93
CPCC12N15/1003C12Q1/6806C12Q1/701C12Q2527/125
Inventor 李刚尹天武汪珂
Owner ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
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