Quetiapine artificial hapten, artificial antigen and preparation method and application thereof

An artificial hapten, artificial antigen technology, applied in chemical instruments and methods, animal/human peptides, animal/human proteins, etc., can solve the problems of artificial hapten and artificial antigen without quetiapine

Active Publication Date: 2020-10-23
HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there are no relevant reports on quetiapine artificial haptens and artificial antigens

Method used

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  • Quetiapine artificial hapten, artificial antigen and preparation method and application thereof
  • Quetiapine artificial hapten, artificial antigen and preparation method and application thereof
  • Quetiapine artificial hapten, artificial antigen and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation method of this implementation a kind of quetiapine artificial antigen (reaction course is as follows: figure 1 ), including the following steps:

[0050] (1) Preparation of artificial hapten:

[0051]① Weigh 200mg (0.45mmol) of quetiapine fumarate into a 50ml conical centrifuge tube, add 20ml of purified water to dissolve, and then add concentrated ammonia water to adjust the pH of the solution to 9. At this time, a large amount of white precipitate appears. Extracted with 20ml of dichloromethane each time, a total of three extractions, collected the organic phase, dried over anhydrous magnesium sulfate, filtered, and evaporated to dryness under reduced pressure to obtain 170mg (0.44mmol) of pale yellow oily substance A;

[0052] The light yellow oily product A was detected by TLC, and the chromatographic solution was dichloromethane: 95% ethanol: 1,4-dioxane: ammonia water=10:8:1:1 (v / v), product Rf= 0.8;

[0053] ②Put 170mg (0.44mmol) of light yello...

Embodiment 2

[0125] The performance measurement of embodiment 2 quetiapine artificial antigen

[0126] (1) Identification of quetiapine artificial antigen:

[0127] Molar absorption coefficient ε: quetiapine artificial hapten solutions with concentrations of 0 μg / ml, 5 μg / ml, 10 μg / ml, 20 μg / ml, 30 μg / ml, and 40 μg / ml were prepared in PBS buffer, and the quetiapine The maximum absorption wavelength of the thiopine hapten is 263nm, the absorbance value is measured at 263nm, and parallel samples are made for each concentration. The formula for calculating the molar absorptivity (ie, the molar absorptivity) is: ε=absorbance value / molar concentration.

[0128] Determination of conjugate protein concentration: Prepare the concentration of 0μg / ml, 10μg / ml, 20μg / ml, 30μg / ml, 40μg / ml, 60μg / ml, 80μg / ml, 100μg / ml, 120μg / ml in PBS buffer Add 3ml of Coomassie Brilliant Blue staining solution to 1ml of bovine gamma globulin solution, mix immediately, warm in a 30°C water bath for 5 minutes, make para...

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PUM

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Abstract

The invention discloses a quetiapine artificial hapten, an artificial antigen as well as a preparation method and application thereof. The molecular structural formula of the quetiapine artificial hapten is shown as a formula (I), and the molecular structural formula of the quetiapine artificial antigen is shown as a formula (II). According to the quetiapine artificial hapten, the characteristic structure of quetiapine is reserved to the maximum extent, and the quetiapine artificial hapten has an active group capable of being coupled with carrier protein and can be used as an antigen determinant; the further prepared quetiapine artificial antigen can be immunized to obtain an anti-quetiapine antibody with high affinity, high sensitivity and strong specificity, the titer of immune ascites obtained by immunizing Balb/c mice reaches up to 1: 78000, and the quetiapine artificial antigen can be used for rapid and accurate immunodetection and immunoassay of quetiapine.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a quetiapine artificial hapten, an artificial antigen, a preparation method and application thereof. Background technique [0002] Quetiapine is an atypical antipsychotic drug that can be used for the maintenance treatment of schizophrenia, acute mania, bipolar depression and bipolar disorder. With its wide application in clinic, the understanding of its adverse reactions is getting deeper and deeper. [0003] According to the investigation report, taking quetiapine will also be accompanied by certain adverse phenomena, common adverse reactions are dizziness, drowsiness, orthostatic hypotension, palpitations, dry mouth, loss of appetite and constipation. It can also cause elevated prolactin levels, weight gain, abdominal pain, asymptomatic elevated transaminases, elevated blood total cholesterol and triglycerides, excitement and insomnia. [0004] At...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D281/16C07K14/47C07K16/44G01N33/53
CPCC07D281/16C07K14/4717C07K16/44G01N33/5308
Inventor 王镇邵越水
Owner HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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