A cotton breeding method utilizing exogenous incompatibility

Abstract

The present application belongs to the technical field of cotton breeding, and specifically relates to a patent application for a cotton breeding method utilizing exogenous incompatibility. The invention utilizes the exogenous poppy self-incompatibility gene pair PrsS / PrpS, and combines it with genetic engineering technology to introduce it into cotton, which proves that introducing the poppy self-incompatibility system into cotton can realize cotton self-incompatibility. Compatibility phenotype, indicating that self-incompatibility can be successfully achieved in cotton (with the transgenic line Gh-PrsS as the male parent and Gh-PrpS as the female parent, cotton bolls cannot be produced normally, and the self-incompatibility of cotton can be achieved and sexuality, that is, the transformation of cotton from self-compatibility to self-incompatibility can be achieved). Using this method, different cotton self-incompatibility new materials can be successfully created, which can greatly save labor costs, improve the purity and efficiency of seed production, and can break through the genotype limitation of materials to achieve high-efficiency seed production. Hybrid cotton cultivation .

Description

technical field [0001] The present application belongs to the technical field of cotton breeding, and specifically relates to a patent application for a cotton breeding method utilizing exogenous incompatibility. Background technique [0002] Cotton is an important economic crop in my country, and its fiber is an essential raw material for my country's textile industry. Cotton is a self-compatible crop and is mostly self-pollinated without outside intervention. From the perspective of cotton breeding, the use of heterosis can greatly increase cotton fiber yield, improve fiber quality, and enhance plant resistance to pests and diseases. [0003] At present, the methods of hybrid seed production of cotton mainly include artificial emasculation, chemical tasseling and male sterility. Large-scale application is difficult. The main reason is that the labor cost of cotton hybrids is too high due to the cumbersome operation during artificial emasculation, which seriously limits ...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for hybrid seed production of cotton mainly include methods such as artificial emasculation, chemical detasseling and male sterile lines, but these methods all have one or more problems such as time-consuming, labor-intensive, unfriendly environment, unstable effect, etc., making Large-scale application is difficult

The main reason is that: during the artificial detasseling of seeds, due to the cumbersome operation, the labor cost of cotton hybrids is too high, which seriously limits the popularization and application of hybrid cotton; and the chemical detasseling method has unstable effects, low application concentration and high application concentration. Time is difficult to control and environmental pollution and other issues; when using male sterile lines for breeding, whether it is the "two-line method" of nuclear male sterility or the "three-line method" of cytoplasmic sterility, it is difficult to select strong Drawbacks of a combination of strengths

Therefore, the high cost of artificial seed production, the unstable fertility of male sterile lines, the narrow source of sterile genes and the poor resilience of restorer lines are the key problems that limit the wide application of cotton hybrids.

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