Cotton breeding method utilizing exogenous self-incompatibility

A cotton-friendly technology, applied in chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of difficult control of application concentration and application time, unstable effect, cumbersome operation, etc.

Active Publication Date: 2020-10-23
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for hybrid seed production of cotton mainly include methods such as artificial emasculation, chemical detasseling and male sterile lines, but these methods all have one or more problems such as time-consuming, labor-intensive, unfriendly environment, unstable effect, etc., making Large-scale application is difficult
The main reason is that: during the artificial detasseling of seeds, due to the cumbersome operation, the labor cost of cotton hybrids is too high, which seriously limits the popularization and application of hybrid cotton; and the chemical detasseling method has unstable effec

Method used

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  • Cotton breeding method utilizing exogenous self-incompatibility
  • Cotton breeding method utilizing exogenous self-incompatibility
  • Cotton breeding method utilizing exogenous self-incompatibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] First of all, it needs to be explained that the main technical idea of ​​this application is to drive the exogenous PrSI stigma-determining gene by constructing a cotton expression vector and using the cotton stigma and pollen tube specific promoter PrsS1 and pollen-determining genes PrpS1 to express. After transforming the expression vector into recipient cotton and obtaining stable homozygous transgenic lines Gh-PrpS and Gh-PrsS, combined with the upland cotton standard line TM-1, reciprocal crossing was carried out by pairwise pairing, and the development of pollen tubes was observed And the statistics of the number of bells, and the actual verification of self-incompatibility.

[0049] In the process of exogenous gene expression, a cotton stigma and pollen tube specific promoter needs to be used, that is, the upstream about 2000 bp sequence of the cotton stigma and pollen tube specific expression gene. Therefore, in this embodiment, a brief introduction to the p...

Embodiment 2

[0063] On the basis of Example 1, the inventors used the plasmid pBI121 as the backbone vector (a commonly used publicly available plasmid), and the exogenous poppy self-incompatibility determining gene pair as the target gene, combined with the obtained in Example 1 Two expression vectors were constructed: the cotton stigma-specific promoter Ghstp was fused with poppy poppy self-incompatibility stigma-determining gene PrsS to construct the stigma-incompatibility expression vector Ghstp-PrsS-pBI121; the pollen tube-specific promoter Ghpop and Pollen incompatibility expression vector Ghpop-PrpS-pBI121 was constructed by fusing the pollen tube determinant gene PrpS of poppy self-incompatibility. The specific construction process is briefly introduced as follows.

[0064] (1) Preparation of poppy self-incompatibility-determining gene pairs

[0065]The PrsS1 (full length 420 bp) and PrpS1 (full length 579 bp) used in this application can be obtained by existing methods such as PC...

Embodiment 3

[0087] On the basis of Example 2, the inventors used the Agrobacterium-mediated transformation technology to further convert the cotton stigma-specific expression vector Ghstp-PrsS1-pBI121 and pollen-incompatible expression vector Ghpop-PrpS-pBI121 in Example 2 into Agrobacterium-mediated The transfer technology was introduced into the recipient cotton Z24, and two groups of stable homozygous self-incompatibility stigma-determining gene and pollen-determining gene transgenic lines Gh-PrpS and Gh were obtained through PCR detection of the target gene and detection of self-fertilization offspring segregation. -PrsS. The specific experimental process is briefly introduced as follows.

[0088] (1) Preparation of transgenic seedlings

[0089] Refer to the existing technology (the specific process is as follows figure 2 shown), cotton seeds (Z24) were germinated and cultured aseptic seedlings, and then the hypocotyls were cut into small sections and then transformed with the stig...

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Abstract

The invention belongs to the technical field of cotton breeding, and particularly relates to a patent application of a cotton breeding method utilizing exogenous self-incompatibility. According to theinvention, an exogenous corn poppy self-incompatibility gene pair PrsS/PrpS is utilized and is introduced into cotton in combination with a genetic engineering technology. The invention proves that:a corn poppy self-incompatible system is introduced into cotton, the cotton self-incompatibility phenotype can be realized, which indicates that the self-incompatibility can be successfully realized in the cotton (the self-incompatibility of the cotton can be realized by taking a transgenic strain Gh-PrsS as a male parent and Gh-PrpS as a female parent, cotton bolls cannot be normally generated, namely, the conversion of the cotton from self-incompatibility to self-incompatibility can be realized). By utilizing the method, different cotton self-incompatible new materials can be successfully created, so that the labor cost is greatly saved, the purity and the efficiency of seed production are improved, the genotype limitation of the materials can be broken through, and the strong-advantagehybrid cotton cultivation of efficient seed production is realized.

Description

technical field [0001] This application belongs to the technical field of cotton breeding, and specifically relates to a patent application for a cotton breeding method utilizing foreign incompatibility. Background technique [0002] Cotton is an important economic crop in my country, and its fiber is an indispensable raw material for my country's textile industry. Cotton is a self-compatible crop, and it mostly self-pollinates without external intervention. From the perspective of cotton breeding, the use of heterosis can greatly increase cotton fiber yield, improve fiber quality, and enhance plant resistance to diseases and insect pests. [0003] At present, the methods for hybrid seed production of cotton mainly include methods such as artificial emasculation, chemical detasseling and male sterile lines, but these methods all have one or more problems such as time-consuming, labor-intensive, unfriendly environment, unstable effect, etc., making Large-scale application i...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12N15/82C12N15/66C12N15/29A01H5/00A01H6/60
CPCC07K14/415C12N15/8231C12N15/8233C12N15/8287C12N15/66
Inventor 李志芳江静王灵璞王鹏宇王朋宝李成程祥飞胡佳敏郑炬瑞迈克·斯科特·杜邦
Owner HENAN UNIVERSITY
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