Cotton breeding method utilizing exogenous self-incompatibility

Abstract

The invention belongs to the technical field of cotton breeding, and particularly relates to a patent application of a cotton breeding method utilizing exogenous self-incompatibility. According to theinvention, an exogenous corn poppy self-incompatibility gene pair PrsS / PrpS is utilized and is introduced into cotton in combination with a genetic engineering technology. The invention proves that:a corn poppy self-incompatible system is introduced into cotton, the cotton self-incompatibility phenotype can be realized, which indicates that the self-incompatibility can be successfully realized in the cotton (the self-incompatibility of the cotton can be realized by taking a transgenic strain Gh-PrsS as a male parent and Gh-PrpS as a female parent, cotton bolls cannot be normally generated, namely, the conversion of the cotton from self-incompatibility to self-incompatibility can be realized). By utilizing the method, different cotton self-incompatible new materials can be successfully created, so that the labor cost is greatly saved, the purity and the efficiency of seed production are improved, the genotype limitation of the materials can be broken through, and the strong-advantagehybrid cotton cultivation of efficient seed production is realized.

Description

technical field [0001] This application belongs to the technical field of cotton breeding, and specifically relates to a patent application for a cotton breeding method utilizing foreign incompatibility. Background technique [0002] Cotton is an important economic crop in my country, and its fiber is an indispensable raw material for my country's textile industry. Cotton is a self-compatible crop, and it mostly self-pollinates without external intervention. From the perspective of cotton breeding, the use of heterosis can greatly increase cotton fiber yield, improve fiber quality, and enhance plant resistance to diseases and insect pests. [0003] At present, the methods for hybrid seed production of cotton mainly include methods such as artificial emasculation, chemical detasseling and male sterile lines, but these methods all have one or more problems such as time-consuming, labor-intensive, unfriendly environment, unstable effect, etc., making Large-scale application i...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for hybrid seed production of cotton mainly include methods such as artificial emasculation, chemical detasseling and male sterile lines, but these methods all have one or more problems such as time-consuming, labor-intensive, unfriendly environment, unstable effect, etc., making Large-scale application is difficult

The main reason is that: during the artificial detasseling of seeds, due to the cumbersome operation, the labor cost of cotton hybrids is too high, which seriously limits the popularization and application of hybrid cotton; and the chemical detasseling method has unstable effects, low application concentration and high application concentration. Time is difficult to control and environmental pollution and other issues; when using male sterile lines for breeding, whether it is the "two-line method" of nuclear male sterility or the "three-line method" of cytoplasmic sterility, it is difficult to select strong Drawbacks of a combination of strengths

Therefore, the high cost of artificial seed production, the unstable fertility of male sterile lines, the narrow source of sterile genes and the poor resilience of restorer lines are the key problems that limit the wide application of cotton hybrids.

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