Silk fibroin 3D printing based biological scaffold and preparation method and application thereof

Abstract

The invention belongs to the field of tissue engineering and medical materials, and relates to a silk fibroin 3D printing based biological scaffold suitable for bronchial mucosa epithelial growth anda preparation method thereof. The biological scaffold is characterized in that silk fibroin/hydroxypropyl carboxymethyl cellulose is adopted as a scaffold material, a 3D printing and freeze drying combined technology is adopted, a bronchial epithelial cell line (BEAS-2B cells) is used as seed cells, the BEAS-2B cells grow on a porous 3D printed silk fibroin/hydroxypropyl carboxymethyl cellulose scaffold, and then the 3D printed silk fibroin/hydroxypropyl carboxymethyl cellulose scaffold is obtained. The silk fibroin 3D printing based scaffold can be used for tracheal defect repair and can provide meaningful reference for development of tissue engineering artificial trachea.

Description

technical field [0001] The invention belongs to the field of tissue engineering and medical materials, and relates to a biological scaffold based on silk fibroin 3D printing and its preparation method and application, in particular to a biological scaffold adapted to the growth of bronchial mucosal epithelium based on silk fibroin 3D printing and its preparation method . Background technique [0002] The trachea is a hollow cylindrical organ lined with a layer of pseudostratified ciliated columnar epithelium that is important for mucus clearance and protection against infection. At present, in clinical treatment, various types of materials such as silicone rubber prosthesis and polyethylene prosthesis are used to repair tracheal defects, but it is difficult to promote the regeneration of airway epithelium and easily lead to complications such as granulation tissue hyperplasia and infection. It is very important to develop scaffolds that can regenerate the epithelium. [00...

AI Technical Summary

Problems solved by technology

At present, bio-3D printing has been widely used in tissue engineering and regenerative medicine, transplantation and clinical research, drug screening and high-throughput analysis, and tumor research; however, not all materials can be used for 3D printing, and the factors affecting 3D printing are more Many, such as the surface tension, viscosity, rheological characteristics and cross-linking mechanism of materials, can affect 3D printing; there are relatively few materials that can be used for biological 3D printing, and most of them are artificially synthesized polymer materials such as polylactic acid and polycaprolactone. Ester, polyglycolic acid, the material can be quickly formed and maintain its specific shape after passing through the printing nozzle, but the biocompatibility of the above materials is poor

The natural polymer materials such as silk fibroin are difficult to solidify and form, so it is difficult to carry out 3D printing. Although there are currently studies on low-temperature printing or cross-linking silk fibroin solution with other substances to form a gel for printing, printing The process is unstable, the requirements for printer equipment are high, or the mechanical properties of the printed scaffold are weak, which cannot meet the needs of tissue repair

[0006] In recent years, some scholars have paid attention to the preparation and characteristics of thixotropic gel; said thixotropic gel means that when the gel is not subjected to shear force, the gel remains in a solid state. force, the gel becomes fluid and is extruded from the syringe, and when the syringe is extruded, the gel quickly returns to a solid state; the hydroxypropyl carboxymethyl cellulose (Hydroxypropyl methylcellulose, HPMC) is a fiber Vegetarian ether derivative, non-toxic, it can form thixotropic gel after mixing and heating with silk fibroin and has good mechanical properties. So far, there is no silk fibroin / hydroxypropyl carboxymethyl fiber at home and abroad. Preparation and Application of 3D Printed Scaffolds for the Growth of Bronchial Epithelial Cells

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