A kind of yeast ry-6 and its application
A technology of RY-6 and yeast, which is applied in the field of environmental microorganisms, can solve problems such as neglect, achieve the effects of reducing odor pollution, improving phosphorus removal efficiency, and practical and economical effects
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Embodiment 1
[0020] Example 1 Saccharomyces RY-6 Isolation and Preservation
[0021] Saccharomyces RY-6 was isolated from the soil samples of Linzhang County, Handan City, Hebei Province through enrichment culture, subculture, primary screening of blue and white spots, secondary screening of heterochromatic particle dyeing, etc.
[0022] The specific method is as follows: collect soil samples at a depth of 0-20 cm in the experimental field of Linzhang County, Handan City, Hebei Province, accurately weigh 1 g and add it to 100 mL enrichment medium, and shake at 28 °C and 180 r / min for 2-3 days to obtain Acclimatized bacteria liquid. After 5 consecutive passages and stabilization, single colonies of different shapes were obtained by gradient dilution plate method, purified by streaking multiple times, transferred to a slant and cultured at 28 °C for 48 h, and stored in a refrigerator at 4 °C for later use.
[0023] The primary screening of phosphorus accumulating bacteria was carried out on...
Embodiment 2
[0034] Example 2 Growth and phosphorus removal characteristics of yeast RY-6 under different initial phosphorus concentrations
[0035] After the yeast RY-6 is activated, adjust the concentration uniformly to 1×10 9 CFU / mL, inoculated at 1% by volume in phosphorus-rich liquid medium with initial phosphorus concentrations of 2, 5, 8, 10, 15, and 20 mg / L, and cultured with shaking at 180 r / min at 28°C for 48–72 h until the bacterial solution becomes turbid. Fermentation broth 8000 r·min -1 After centrifugation for 10 min, the supernatant was taken and passed through a 0.22 μm filter membrane to measure the mass concentration of phosphorus in the culture solution before and after cultivation, and calculate the phosphorus removal rate of each strain.
[0036] test methods:
[0037] (1) Determination of bacterial concentration: turbidimetric method, the absorbance value OD at 600 nm by spectrophotometer 600 To represent the cell concentration of bacteria.
[0038] (2) Total p...
Embodiment 3
[0043] Example 3 Saccharomyces RY-6 Deodorization Ability Determination
[0044] Take 18 mL of bacterial liquid in a 2 L large beaker, and add 2 mL of 500 mg / L ammonia water; then put a 50 mL small beaker containing 20 mL of 0.005 N sulfuric acid absorption solution into the large beaker, and cover with a double layer Plastic film seal. The same amount of sterile water was used as the control, repeated three times, and cultured in a 28 °C incubator. After 24 h, the small beaker was taken out to detect the concentration of ammonia.
[0045] Ammonia determination method: Nessler's reagent colorimetric method.
[0046] Ammonia removal rate: Ammonia removal rate = (ammonia concentration in control absorption solution - ammonia concentration in treatment absorption solution) / ammonia concentration in control absorption solution × 100%. The results showed that the deamination rate of yeast RY-6 reached over 65% within 24 h.
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