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Bifidobacterium animalis with bowel relaxing function as well as application and preparation of bifidobacterium animalis

A technology for animal bifidobacteria and bowel laxatives, applied in bifidobacteria, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. The high cost of yogurt can achieve the effect of good laxative function, enhanced oxygen tolerance and broad application prospects.

Active Publication Date: 2020-10-27
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on probiotics in my country started relatively late, and the mature and stable industrial preparations of probiotics are basically monopolized by foreign strain companies, resulting in a situation where the cost of probiotic yoghurt is relatively high and products are overly dependent on strain suppliers.

Method used

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  • Bifidobacterium animalis with bowel relaxing function as well as application and preparation of bifidobacterium animalis
  • Bifidobacterium animalis with bowel relaxing function as well as application and preparation of bifidobacterium animalis
  • Bifidobacterium animalis with bowel relaxing function as well as application and preparation of bifidobacterium animalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example provides a Bifidobacterium animalis MN-Gup, the preservation number of which is CGMCC No.15578.

[0024]In the present invention, Bifidobacterium animalis MN-Gup is obtained by strain isolation and aerospace mutagenesis of bacterial strains. Specifically, (1) the separation of bacterial strains is as follows: collecting intestinal contents of healthy and long-lived elderly people in Bama, Guangxi, and That is feces, the intestinal feces samples of the healthy and long-lived elderly in Bama, Guangxi were gradiently diluted with diluent, wherein, the preparation method of diluent: sodium dihydrogen phosphate 4.5g, disodium hydrogen phosphate 6.0g, L-cysteine ​​0.5 g, agar 0.5g, Tween 80 0.5ml, distilled water 1000ml, heat to dissolve, adjust the pH to 7.4-7.6, sterilize at 121°C for 15min, and use NPNL-X-Gal selective medium (containing neomycin sulfate , Paromomycin Sulfate, Sodium Propionate, Lithium Chloride) anaerobically isolate Bifidobacteria (Yang Haiy...

Embodiment 2

[0026] This embodiment provides a preparation, including Bifidobacterium animalis MN-Gup and inulin. Based on every gram of the preparation, the content of Bifidobacterium animalis MN-Gup is 4×10 6 CFU / g, the content of described inulin is 10mg / g;

[0027] The preparation method of above-mentioned preparation, comprises the steps:

[0028] (1) Preparation of bacterial suspension: culture the Bifidobacterium animalis MN-Gup strain with MRS liquid culture at 37°C for 12 hours, centrifuge at 4500r / min for 15min, and resuspend the bacterial pellet in normal saline , formulated as 8 x 10 6 CFU / g of bacterial suspension;

[0029] (2) Configuration of inulin: accurately weigh 0.2 g of inulin and dissolve it in 10 g of physiological saline (20 mg / g) to obtain inulin solution;

[0030] (3) Take the equal mass of the bacterial suspension and mix the inulin solution to obtain the preparation.

Embodiment 3

[0032] This embodiment provides a preparation, including Bifidobacterium animalis MN-Gup and inulin. Based on every gram of the preparation, the content of Bifidobacterium animalis MN-Gup is 4×10 6 CFU / g, the content of described inulin is 25mg / g.

[0033] The preparation method of above-mentioned preparation, comprises the steps:

[0034] (1) Preparation of bacterial suspension: culture the Bifidobacterium animalis MN-Gup strain with MRS liquid culture at 37°C for 12 hours, centrifuge at 4500r / min for 15min, and resuspend the bacterial pellet in normal saline , formulated as 8 x 10 6 CFU / g of bacterial suspension;

[0035] (2) Configuration of inulin: accurately weigh 0.5 g of inulin and dissolve it in 10 g of physiological saline (50 mg / g), to obtain inulin solution;

[0036] (3) Take the equal mass of the bacterial suspension and mix the inulin solution to obtain the preparation.

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Abstract

The invention discloses Bifidobacterium animalis with a bowel relaxing function and application and a preparation of the Bifidobacterium animalis. According to the Bifidobacterium animalis, Bifidobacterium lactis BB-11 is separated from an intestinal content of healthy and long-lived elderly people in Bama, Guangxi, the strain is carried on a spacecraft, finally, a Bifidobacterium animalis MN-Gupwith significantly improved oxygen resistance or significantly enhanced oxygen resisting capacity is screened by space mutagenesis, and evaluation of constipation model mice proves that the strain hasthe good bowel relaxing function and has broad application prospects in bowel relaxing drugs, health foods and foods.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an animal bifidobacterium (Bifidobacterium animalis) for laxative and laxative, as well as its application and preparation. Background technique [0002] In recent years, the yogurt market has developed rapidly and continued to grow at a double-digit growth rate. In 2018, the overall growth rate of yogurt slowed down. However, it can be seen from the development trend of the yogurt market segment that functional yogurt is being sought after by more and more consumers. Moreover, when analyzing the development of the yogurt market in developed countries, it can be seen that functional yogurt in the Japanese market is an important reason for the continuous growth of the entire Japanese yogurt market. Therefore, functional yogurt is an important sub-category for future yogurt market development. [0003] With the continuous acceleration of the pace of modern life, people's ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L11/00A23L2/385A23L2/38A23G9/36A23G3/36A23C9/123A21D8/04A61K35/745A61K47/36A61P1/00A61P1/10C12R1/01
CPCA23L33/135A23C9/1234A23G9/363A23G3/366A61K35/745A61P1/10A23V2002/00A61K31/733C12N1/20C12N1/02A23L33/21A23L2/39A23L11/00A23L2/52A23L2/02A21D13/06A61K2300/00A23V2200/3204A23V2250/5062
Inventor 孙二娜孙健牛天娇康小红赵红峰陈建国姚斐邓凤生赵伊凡刘巨龙江雷
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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