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A kind of bifidobacterium lactis for laxative and its application and preparation

A technology of Bifidobacterium lactis and bowel laxative, which is applied in the direction of Bifidobacterium, medical preparations with no active ingredients, medical preparations containing active ingredients, etc., which can solve the transitional dependence of products on strain suppliers, probiotics The problem of high cost of yogurt can achieve the effect of good bowel laxative function, enhanced oxygen resistance and broad application prospects

Active Publication Date: 2022-06-21
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on probiotics in my country started relatively late, and the mature and stable industrial preparations of probiotics are basically monopolized by foreign strain companies, resulting in a situation where the cost of probiotic yoghurt is relatively high and products are overly dependent on strain suppliers.

Method used

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  • A kind of bifidobacterium lactis for laxative and its application and preparation
  • A kind of bifidobacterium lactis for laxative and its application and preparation

Examples

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Comparison scheme
Effect test

Embodiment 1

[0023] This example provides a Bifidobacterium animalis MN-Gup, whose deposit number is CGMCC No.15578.

[0024]In the present invention, Bifidobacterium animalis MN-Gup is obtained by bacterial strain isolation and spaceflight mutagenesis of the bacterial strain. Specifically, (1) the isolation of the bacterial strain is: collecting the intestinal contents of healthy and long-lived elderly people in Bama, Guangxi, namely, Stool, use dilution liquid to dilute the intestinal stool samples of Guangxi Bama healthy and long-lived elderly, wherein, the preparation method of dilution liquid: sodium dihydrogen phosphate 4.5g, disodium hydrogen phosphate 6.0g, L-cysteine ​​0.5g , agar 0.5g, Tween 80 0.5ml, distilled water 1000ml, heat to dissolve, adjust pH to 7.4-7.6, sterilize at 121°C for 15min, spare, use NPNL-X-Gal selective medium (containing neomycin sulfate, Paromomycin sulfate, sodium propionate, lithium chloride) anaerobic separation of bifidobacteria (Yang Haiying can be us...

Embodiment 2

[0026] This example provides a formulation comprising Bifidobacterium animalis MN-Gup and inulin. Based on the preparation per gram, the content of Bifidobacterium animalis MN-Gup is 4×10 6 CFU / g, the content of the inulin is 10mg / g;

[0027] The preparation method of above-mentioned preparation, comprises the steps:

[0028] (1) Preparation of bacterial suspension: Bifidobacterium animalis MN-Gup strain was cultured at 37°C for 12h with MRS liquid culture, and centrifuged at 4500r / min for 15min, and the bacterial pellet was resuspended in normal saline , formulated as 8×10 6 CFU / g bacterial suspension;

[0029] (2) Configuration of inulin: accurately weigh 0.2 g of inulin and dissolve it in 10 g of physiological saline (20 mg / g) to obtain an inulin solution;

[0030] (3) Mix the bacterial suspension of equal quality with the inulin solution to obtain the preparation.

Embodiment 3

[0032] This example provides a formulation comprising Bifidobacterium animalis MN-Gup and inulin. Based on the preparation per gram, the content of Bifidobacterium animalis MN-Gup is 4×10 6 CFU / g, the content of the inulin is 25mg / g.

[0033] The preparation method of above-mentioned preparation, comprises the steps:

[0034] (1) Preparation of bacterial suspension: Bifidobacterium animalis MN-Gup strain was cultured at 37°C for 12h with MRS liquid culture, and centrifuged at 4500r / min for 15min, and the bacterial pellet was resuspended in normal saline , formulated as 8×10 6 CFU / g bacterial suspension;

[0035] (2) Configuration of inulin: accurately weigh 0.5 g of inulin and dissolve it in 10 g of physiological saline (50 mg / g) to obtain an inulin solution;

[0036] (3) Mix the bacterial suspension of equal quality with the inulin solution to obtain the preparation.

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Abstract

The invention discloses a laxative Bifidobacterium lactis and its application and preparation. The Bifidobacterium animalis was isolated from the intestinal contents of healthy and long-lived elderly in Bama, Guangxi to obtain Bifidobacterium lactis BB‑11, carried the strain into spaceflight, and finally obtained a resistant strain through space mutagenesis. Bifidobacterium animalis MN‑Gup with significantly improved oxygen performance, that is, has significantly enhanced oxygen tolerance, and the evaluation of constipation model mice has proved that the strain has a good bowel laxative function, and it is used in moistening It has broad application prospects in bowel laxative medicine, health food and food.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Bifidobacterium animalis for laxative bowel movement and application and preparation thereof. Background technique [0002] In recent years, the yogurt market has developed rapidly and continued to grow at a double-digit growth rate. In 2018, the growth rate of yogurt slowed down as a whole. However, it can be seen from the development trend of yogurt market segments that functional yogurt is being sought after by more and more consumers. Moreover, when analyzing the development of the yogurt market in developed countries, it can be seen that functional yogurt in the Japanese market is an important reason for the continuous growth of the entire Japanese yogurt market. Therefore, functional yogurt is an important subcategory for the future development of the yogurt market. [0003] With the continuous acceleration of the pace of modern life, people's diet has entered the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L11/50A23L2/385A23L2/38A23G9/36A23G3/36A23C9/123A21D8/04A61K35/745A61K47/36A61P1/00A61P1/10C12R1/01
CPCA23L33/135A23C9/1234A23G9/363A23G3/366A61K35/745A61P1/10A23V2002/00A61K31/733C12N1/20C12N1/02A23L33/21A23L2/39A23L11/00A23L2/52A23L2/02A21D13/06A61K2300/00A23V2200/3204A23V2250/5062
Inventor 孙二娜孙健牛天娇康小红赵红峰陈建国姚斐邓凤生赵伊凡刘巨龙江雷
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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