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Biological agent for increasing adaptive breeding survival rate of young litopenaeus vannamei

A technology of biological agents and white shrimp, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as neglect, poor application effect, and breeding failure

Inactive Publication Date: 2020-10-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological agents in the field of prawns adjust the breeding environment from the perspective of "individual microorganisms". Lead to poor application effect or even breeding failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Application case of indoor standard thickness and high density of prawns. This embodiment includes component A and component B, wherein component A is Thiobacillus thiooxidans. Component B is Steiner's denitrifying bacteria, the applicable amount of component A is 2L, component B is 1L, and the aquaculture water body is 200m 2 .

[0019] The expansion method of component A: 100 g of component A is cultivated in 100 L of nutrient solution. In component A, 70 g of pure bacteria powder after freeze-drying is mixed with 15 g of sodium alginate and 15 g of skimmed milk powder. The nutrient solution contains: Sodium sulfate 1000 mg / L, magnesium sulfate heptahydrate 50 mg / L, potassium dihydrogen phosphate 15 mg / L, calcium bicarbonate 250 mg / L, calcium carbonate 500 mg / L, culture water temperature 25-35 ℃, culture time 48 -72 hours.

[0020] The expansion method of component B: 50 g of component B is cultivated in 200 L of liquid, in component B, 35 g of pure bacteria powder...

Embodiment 2

[0026] An application case of thick and high-density marking in indoor cement ponds for prawns. This embodiment includes component A and component B, wherein component A is Thiobacillus thiooxidans. Component B is Steiner's denitrifying bacteria, the applicable amount of component A is 1.5L, component B is 2L, and the aquaculture water body is 300m 2 .

[0027] Table 2 is the effect that this embodiment is applied to prawn elevated pond culture:

[0028] Table 2

[0029] project value Breeding survival rate a

[0030] .

Embodiment 3

[0032] An application case of thick and high-density labeling in shrimp sheds. This embodiment includes component A and component B, wherein component A is Thiobacillus thiooxidans. Component B is Steiner's denitrifying bacteria, the applicable amount of component A is 1.5L, component B is 2L, and the aquaculture water body is 250m 2 .

[0033] Table 3 is the effect that this embodiment is applied to prawn elevated pond culture:

[0034] table 3

[0035] project value Breeding survival rate a

[0036] .

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PUM

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Abstract

The invention discloses a biological agent for increasing the adaptive breeding survival rate of young litopenaeus vannamei. The biological agent mainly comprises a component A and a component B, wherein the component A is thiobacillus thiooxidans, and the component B is aerobic denitrifying bacteria. According to the biological agent, the component B is firstly used, and meanwhile the component Ais used in a delayed mode. The biological agent can improve the redox potential of the bottom of a breeding environment, and reduces harmful flora in the environment based on the principles of microbial ecological occupancy, microbial matrix competition and the like through the synergistic sequence application of the component A and the component B, thereby basically eradicating the generation oftoxic substances such as hydrogen sulfide, ammonia nitrogen and nitrite, and further improving the survival rate of the bred litopenaeus vannamei. Compared with conventional biological agent and chemical agent, the biological agent has the advantages of quick effect taking and long duration.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and specifically relates to a compound biological agent preparation technology and a use method for improving the survival rate of prawn seedlings by improving the environment for raising prawn seedlings. Background technique [0002] Litopenaeus vannamei, Litopenaeus vannamei It has become an important aquaculture species in my country. It also provides effective support to ensure the safety of the vegetable basket. [0003] However, due to the large amount of high-protein feed fed during the breeding process, it is very easy to cause toxic ammonia nitrogen, hydrogen sulfide, nitrite and other substances in the breeding environment. Due to its own physiological characteristics, the bottom environment is the main habitat for prawns, and the foraging, movement, and shelling of prawns are all completed at the bottom. If the bottom environment deteriorates, it will inevitably lead to an incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/59A01K61/13C12R1/01C12R1/38
CPCA01K61/13A01K61/59C12N1/20Y02A40/81
Inventor 阮贇杰周凡丁雪燕马文君孔德栋殷旭旺陆慧锋
Owner ZHEJIANG UNIV
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