Livestock and poultry manure deodorizing microbial agent as well as preparation method and application thereof

A technology for microbial inoculum and livestock manure, applied in the field of microorganisms, can solve the problems of limited area of ​​action, secondary pollution, expensive drugs, etc., and achieve the effects of simple usage, long life cycle and low cost

Inactive Publication Date: 2020-11-03
陕西均良土壤环境技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical deodorization can be achieved by applying strong oxidizing substances to feces to degrade odorous substances, using mixed aromatic compounds to cover odors, and using adsorbents (peat, zeolite) to absorb odorous substances, although the effect is quick , strong purpose but expensive medicines, large amount of spraying per unit, limited area of ​​action, and sometimes the residue of chemical agents will cause secondary pollution

Method used

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  • Livestock and poultry manure deodorizing microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The preparation of the microbial bacterial agent of the embodiment of the present invention comprises:

[0066] (1) Activate Bacillus subtilis, Lysinobacillus pakistani, Lysinobacillus borate, and Bacillus ginseng in LB solid slant medium respectively, and culture Aspergillus niger and Phalaenopsis fungus on PDA solid slant respectively Activation in base, LB solid medium formula: tryptone 10g, yeast extract powder 5g, sodium chloride 10g, deionized water 1000mL, agar 15-20g, pH 7.0, PDA solid medium formula: potato 200g, glucose 20g , agar 15-20g, water 1000mL, pH value is natural.

[0067] (2) The activated Bacillus subtilis, Lysinobacillus pakistani, Lysinobacillus borate, and Bacillus ginseng were respectively inoculated into sterilized LB liquid medium, and cultivated in a constant temperature incubator at 37°C for 12 hours. The activated Aspergillus niger and Phalaenopsis fungus were respectively inoculated into PDA liquid medium for culture, and cultured in a co...

Embodiment 2

[0071] The application of a kind of microbial bacterial agent of the embodiment of the present invention comprises:

[0072] Add 3kg of fresh pig manure, 3kg of sawdust, 200g of corn bran and 6g of urea into a barrel reactor with a height of 40cm and a diameter of 35cm; add 250g of self-made solid microbial agent to the first group of barrels, and add Commercially available bacterium agent 250g, the third group does not add other substances and is used as a blank control group, and each group of experiments is set up three times again, measure the indole concentration, skatole concentration, ammonia concentration after 18 days, the measurement results are shown in the following table 1 .

[0073] Table 1: Deodorizing effect of bacteria agent

[0074]

[0075] It can be seen from Table 1 that compared with the control group, the indole concentration, skatole concentration, and ammonia gas concentration of the two groups added with bacterial agents all decreased significantl...

Embodiment 3

[0077] A microbial agent for deodorizing poultry and livestock manure according to an embodiment of the present invention, said microbial agent is uniformly prepared by mixing protein-degrading bacterial strains, starch-degrading bacterial strains, and cellulose-degrading bacterial strains;

[0078] Among them, the protein-degrading strain is Bacillus subtilis; the starch-degrading strain is Aspergillus niger; the cellulose-degrading strain is Phalaenopsis.

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Abstract

The invention discloses a livestock and poultry manure deodorizing microbial agent as well as a preparation method and application thereof. The microbial agent is prepared by uniformly mixing a protein degradation strain with a starch degradation strain and a cellulose degradation strain, wherein the protein degradation strain is one or two of bacillus subtilis and Pakistan lysine bacillus; the starch degradation strain is one or two of aspergillus niger and lysine borate bacillus; and the cellulose degradation strain is one or two of botrytis fungi and panax ginseng bacillus. Screened proteindegradation microorganisms, starch degradation microorganisms and cellulose degradation microorganisms are adopted to prepare a complex microbial inoculant, the problem of stink is solved by using the complex microbial inoculant as a feed additive or directly using the complex microbial inoculant for stink excrement and the like. The livestock and poultry manure deodorizing microbial agent is simple in use method, remarkable in effect, low in cost, and is safe and environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial agent for deodorizing poultry manure and its preparation method and application. Background technique [0002] With the rapid development of large-scale breeding industry, a large amount of poultry manure will be produced every year. If these substances are not properly treated, they will cause odor pollution. Toxic malodorous gases mainly including ammonia, hydrogen sulfide, and indole will not only cause people's unpleasantness through the olfactory system, but also strongly stimulate the respiratory, nervous, circulatory, endocrine and other systems of people who are frequently in the odorous environment, and even Some people have adverse effects such as loss of smell, anorexia, nausea, and physical damage. In addition, the malodorous gas entering the atmosphere may form acid rain and cause more serious environmental pollution. Therefore, resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F11/02A23K10/18C02F103/20C12R1/125C12R1/01C12R1/685C12R1/07C12R1/645
CPCC12N1/20C12N1/14C02F11/02A23K10/18C02F2103/20
Inventor 李宇刘永军张至张驰白慧
Owner 陕西均良土壤环境技术有限责任公司
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