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Tumor-targeted photoacoustic imaging-guided multi-stage therapy nanoprobe and preparation method

A nanoprobe, photoacoustic imaging technology, applied in the field of biomedical nanomaterials, can solve problems such as poor penetration ability, and achieve the effect of increasing the intake

Active Publication Date: 2022-05-17
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, domestic and foreign researches on nanoprobes that use albumin biomineralization to integrate photoacoustic imaging and photothermal therapy functions of NIR-II, and have tumor-targeted nanoprobes assisted by starvation therapy and chemodynamic therapy. There are few studies on integrated probes for diagnosis and treatment, and they are mainly limited to the research on the function of nanoprobes in the near-infrared region with poor penetrating ability.

Method used

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  • Tumor-targeted photoacoustic imaging-guided multi-stage therapy nanoprobe and preparation method
  • Tumor-targeted photoacoustic imaging-guided multi-stage therapy nanoprobe and preparation method
  • Tumor-targeted photoacoustic imaging-guided multi-stage therapy nanoprobe and preparation method

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preparation example Construction

[0036] A method for preparing a tumor-targeted photoacoustic imaging-guided multi-stage treatment integrated nanoprobe, comprising the following steps:

[0037] S1. According to the mass ratio of bovine serum albumin: deionized water = 100:3, add bovine serum albumin into deionized water, ultrasonically disperse for 15 minutes, and stir at room temperature for 15 minutes to form a transparent bovine serum albumin aqueous solution;

[0038] S2. According to the molar ratio of inorganic copper source: nitric acid = 48.32mg: 1mL, add the solid inorganic copper source into 2mol / L nitric acid solution, ultrasonically disperse it for 5 minutes, then quickly add it to the bovine serum albumin aqueous solution in S1, and stir vigorously for 10 minutes ;

[0039] S3, adding dropwise a sodium hydroxide solution with a concentration of 2 mol / L to the mixed solution in S2, adjusting the pH of the mixed solution to be 12;

[0040] S4. Take 0.5 mL of sodium sulfide aqueous solution with a ...

Embodiment 1

[0047] Take 138.6mL of nitric acid with a concentration of 65% and 806mL of deionized water, put them into a beaker and mix them thoroughly, transfer the mixed solution to a volumetric flask, and prepare a nitric acid solution with a concentration of 2moL / L for later use;

[0048] Take 83.33g of sodium hydroxide and 1L of deionized water, add them into a beaker and mix them thoroughly, transfer the mixed solution to a volumetric flask, and prepare a sodium hydroxide solution with a concentration of 2moL / L for later use;

[0049] Take 24.22g of sodium sulfide nonahydrate and 1L of deionized water, add them together to a beaker and mix thoroughly, transfer the mixed solution to a volumetric flask, and prepare a 0.1mol / L sodium sulfide solution for later use;

[0050] Take 105mL of deionized water and 3.75g of bovine serum albumin, add them together into a beaker, place them in an ultrasonic disperser for dissolution and dispersion, the frequency is 50KHz, the dispersion time is 1...

Embodiment 2

[0064] Take 138.6mL of nitric acid with a concentration of 65% and 806mL of deionized water, put them into a beaker and mix them thoroughly, transfer the mixed solution to a volumetric flask, and prepare a nitric acid solution with a concentration of 2moL / L for later use;

[0065] Take 83.33g of sodium hydroxide and 1L of deionized water, add them into a beaker and mix them thoroughly, transfer the mixed solution to a volumetric flask, and prepare a sodium hydroxide solution with a concentration of 2moL / L for later use;

[0066]Take 24.22g of sodium sulfide nonahydrate and 1L of deionized water, add them together to a beaker and mix thoroughly, transfer the mixed solution to a volumetric flask, and prepare a 0.1mol / L sodium sulfide solution for later use;

[0067] Take 105mL of deionized water and 3.75g of bovine serum albumin, add them together into a beaker, place them in an ultrasonic disperser for dissolution and dispersion, the frequency is 50KHz, the dispersion time is 15...

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Abstract

The invention relates to the technical field of biomedical nanomaterials, in particular to a tumor-targeted photoacoustic imaging-guided multi-stage therapeutic nanoprobe and a preparation method thereof. The probe is based on bovine serum albumin-coated copper sulfide nanoparticles with uniform dispersion and negative surface charge synthesized by bovine serum albumin biomineralization method. Encapsulation of positively charged L-polylysine and negatively charged glucose oxidase mixed hyaluronic acid layer to provide a nanoprobe with photoacoustic imaging and multi-stage therapy, the material is in the tumor microenvironment It can rely on hyaluronic acid to actively target the abundant CD44 receptors on the surface of triple-negative breast cancer cell membranes, release glucose oxidase for starvation therapy, flip the surface charge of nanoparticles, increase its cell uptake rate, and further perform photothermal therapy and Chemodynamic therapy to enable and enhance imaging and therapeutic performance of nanoprobes.

Description

technical field [0001] The invention relates to the field of biomedical nanomaterials, more specifically, to a tumor-targeted photoacoustic imaging-guided multi-stage therapeutic nanoprobe and a preparation method. Background technique [0002] Breast cancer is a tumor with high mortality among women worldwide, especially triple-negative breast cancer (TNBC), which is a highly aggressive and metastatic subtype of breast cancer. With the increasing development of nanomaterials, the development of nanoplatforms for targeting, diagnosis and treatment of TNBC is an urgent need for effective treatment. [0003] Due to the severe hypoxia and low pH microenvironment, cancer cells are more sensitive to temperature increase than normal cells. Photothermal therapy (PTT), as a new type of physical therapy, has attracted increasing attention in the field of cancer treatment. Nanoparticle-mediated PTT uses photothermal agents to effectively ablate cancer cells and tissues by converting...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K47/69A61K47/61A61K47/64A61K38/44A61K41/00A61P35/00
CPCA61K49/225A61K47/6931A61K47/6939A61K47/6935A61K47/61A61K47/643A61K47/645A61K41/0052A61K38/443C12Y101/03004A61P35/00A61K2300/00
Inventor 张瑞平郑子良陈琪秦宇飞段艾林
Owner SHANXI MEDICAL UNIV