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A method of utilizing molasses to produce biological feed with the function of replacing antibiotics

A technology of biological feed and molasses, applied in the field of agricultural biology, can solve problems such as unclear specific reasons, and achieve the effect of reducing production cost and improving conversion rate

Active Publication Date: 2022-05-31
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when molasses is converted by commercially available fructosyltransferase, the conversion rate of sucrose in molasses is usually 25-30%, which is far lower than that of pure sucrose (conversion rate is 80-85%). The specific reasons It is not yet clear, which is the fundamental reason why molasses has not adopted this scheme for mass production

Method used

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  • A method of utilizing molasses to produce biological feed with the function of replacing antibiotics

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the effect of fructosyltransferase on the conversion rate of sucrose in molasses

[0022] The fructosyltransferase currently used commercially is the fructosyltransferase derived from Aspergillus niger, and its gene sequence is shown in the NCBI database ABB59678.1, and the optimum temperature is 45-50°C. The fructosyltransferase FRC1 in the present invention The similarity is 64.4%, and the optimum temperature is 70-75 °C. Using molasses as raw material, the commercial enzyme activity is calibrated at 50 °C, and the FRC1 is calibrated at 75 °C. When the addition amount is 1000U / L, respectively After 12 and 24 hours of conversion at the optimum temperature, the conversion of molasses by commercial enzymes was 22.4% and 23.6%, respectively, and the conversion of molasses by FRC1 was 64.2% and 67.4%, respectively.

Embodiment 2

[0023] Embodiment 2, the preparation method of biological feed additive with the function of substituting antibiotics

[0024] (1) In a 2-ton reaction kettle, weigh 1 ton of beet molasses with a dry matter content of 70%, in which the sucrose content is 46%, add 400 kg of water, start stirring to mix the materials evenly, and use 10% hydrogen peroxide. Potassium adjusts pH to 6.5. Then adjust the stirring speed at 60-80 r / min, gradually heat up to 75 °C, add 1 kg of fructosyltransferase with an enzyme activity of 1500 U / mL, and keep the temperature at 75 °C for 12 hours. , the fructooligosaccharide content was 18.2%. The enzymatic activity determination of fructosyltransferase and the content determination of fructooligosaccharides were performed according to GBT-23528-2009.

[0025] (2) After reducing the temperature to 45°C, add 80-100 kg of calcium carbonate, stir evenly, and then add 1 kg of 10,000 U / mL glucose oxidase and 200,000 U / mL catalase in sequence, with a ventil...

Embodiment 3

[0030] Example 3. Effect verification of biological feed with the function of replacing antibiotics in broiler experiments

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Abstract

The invention relates to the field of agricultural biotechnology, in particular to a method for using molasses to produce biological feed with the function of replacing antibiotics. The shown method comprises the step of using fructosyltransferase FRC1 to convert fructo-oligosaccharides by using molasses, a by-product of agricultural product processing, as a raw material. The functional biological feed produced by the present invention can be used with other full-price feeds according to the addition amount of 0.5%-1%, and the biological feed produced by using this technology, when the addition amount is 1%, died in the broiler feeding test. The washing rate, feed-to-meat ratio and daily growth were significantly better than those of the control group, and there was no significant difference compared with the antibiotic group, indicating that it can be an effective substitute for antibiotics.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for producing biological feed with the function of replacing antibiotics by using molasses. Background technique [0002] For a long time, the abuse of antibiotics in feed has brought serious food safety problems, and the research on antibiotic substitutes has been paid more and more attention. Functional oligosaccharides have the characteristics of no pollution, no residue, participate in intestinal metabolism in the body, and maintain the microecological balance of the host. They are an ideal feed additive that can replace antibiotics. The role of fructooligosaccharides in livestock and poultry breeding is mainly manifested in the selective proliferation of beneficial bacteria such as bifidobacteria, probiotics proliferate in large quantities, generate acidic substances to reduce intestinal pH, inhibit the growth of harmful bacteria, and improve animal disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/33A23K10/14
CPCA23K10/33A23K10/14Y02P60/87
Inventor 柏映国姚斌罗会颖涂涛王亚茹黄火清苏小运王苑师霞
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI