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Method for determining content of four alkaloids of uncaria rhynchophylla in Qingda granules

A technology of alkaloids and isorhynchophylline, which is applied in the direction of measuring devices, anti-vector-borne diseases, instruments, etc., to achieve the effect of simple method, reducing solvent loss and solvent consumption

Active Publication Date: 2020-11-13
JIANGYIN TIANJIANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is no report on the content determination of the four alkaloids of Uncaria uncaria in the Qingda granule system

Method used

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  • Method for determining content of four alkaloids of uncaria rhynchophylla in Qingda granules
  • Method for determining content of four alkaloids of uncaria rhynchophylla in Qingda granules
  • Method for determining content of four alkaloids of uncaria rhynchophylla in Qingda granules

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Experimental program
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preparation example Construction

[0065]

[0066] Qingda Granules are produced from Qingxuan Jiangya Decoction, and are mainly prepared from raw materials with the following weight ratio: Gastrodia elata 10-30 parts, Uncaria 10-20 parts, Lianzixin 5-15 parts, Scutellaria baicalensis 5 parts -15 copies. Qingda granules are mainly used for prehypertension or grade 1 hypertension. Gastrodia elata is used as the king to calm the liver and subdue yang, supplemented by Uncaria elata, to help Gastrodia elata clear heat, calm the liver and calm wind, and add Scutellaria baicalensis to clear liver heat, and lotus seed core to vent the heart fire, thereby The floating yang of the liver is hidden, and the heat of the heart and liver is reduced, so as to subdue the yang and calm the liver, restrain the wind, lower the blood pressure, and recover the damage of the heart, brain and kidney. Uncaria in the prescription has multiple functions such as lowering blood pressure, calming, and anti-oxidation. The main active ingr...

Embodiment

[0104] 1. Experimental materials:

[0105] 1.1 Test drug:

[0106] 1.1.1 Reference substance (for content determination, no treatment is required before use):

[0107] Rhynchophylline, batch number: 112028-201601, purchased from China Institute for Food and Drug Control;

[0108] Isorhynchophylline, batch number: 11927-201403, purchased from China Institute for Food and Drug Control;

[0109] Dehydrorhynchophylline, product number: ST10570120MG 630-94-4, purity ≥ 98.0%, purchased from Shanghai Shidande Biotechnology Co., Ltd.;

[0110] Isodehydrorhynchophylline, product number: ST11080120MG 51014-29-0, purity ≥ 98.0%, purchased from Shanghai Shidande Biotechnology Co., Ltd.

[0111] 1.1.2 Test product

[0112] Batch numbers of Qingda Granules: 1905310, 1905001, 1905002, 1905003, provided by Jiangyin Tianjiang Pharmaceutical Co., Ltd.

[0113] 1.1.3 Other reagents:

[0114] Methanol, superior grade;

[0115] Acetonitrile, chromatographically pure;

[0116] Ammonia, chro...

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Abstract

The invention provides a method for determining the content of four alkaloids of uncaria rhynchophylla in Qingda granules. The method comprises the following steps: preparing a test solution, preparing a reference solution and determining by adopting an ultra-high performance liquid chromatograph, wherein the preparation of the test solution comprises the following steps: taking Qingda granules under a loading amount difference item; grinding, precisely weighing, adding methanol, weighing, carrying out ultrasonic treatment, cooling, weighing again, supplementing the reduced weight with methanol, shaking up, filtering, precisely absorbing subsequent filtrate, passing through an alkaline alumina column, adding an elution solvent for elution, collecting effluent and eluent, drying by distillation, adding a redissolving solvent into residues, dissolving, and shaking up. The method provided by the invention solves the problem of full extraction of uncaria related components from Qingda granules, reduces the damage of impurities to a chromatographic column in the detection process, and also can realize qualitative research of lotus plumule detection.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for determining the contents of four alkaloids in Uncaria granules. Background technique [0002] Hypertension is a common cardiovascular disease, and its complications are one of the main causes of human death. In recent years, the prevalence of hypertension has risen rapidly, and its prevention and treatment are urgently needed. At present, drug therapy is the most important treatment method for hypertension, among which Chinese medicine treatment has gradually become a hot spot in the early prevention and treatment of hypertension (Xu Hongye, Wang Youqun. Research and application of Chinese medicine in the treatment of hypertension [J]. China Practical Medicine, 2008, 3(25):189-192). Academician Chen Keji, a master of traditional Chinese medicine, believes that the onset of hypertension has an evolutionary process from excess of yang to deficiency of both yin an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679Y02A50/30
Inventor 王协和陈盛君陈可冀李松范恺磊杜俊潮
Owner JIANGYIN TIANJIANG PHARMA