Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of content determination method of four kinds of alkaloids of Uncaria in Qingda granules

A technology of alkaloids and granules, which is used in measuring devices, resistance to vector-borne diseases, instruments, etc., to achieve the effect of simple method, reducing interference, and improving detection standards

Active Publication Date: 2022-08-09
JIANGYIN TIANJIANG PHARMA
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is no report on the content determination of the four alkaloids of Uncaria uncaria in the Qingda granule system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of content determination method of four kinds of alkaloids of Uncaria in Qingda granules
  • A kind of content determination method of four kinds of alkaloids of Uncaria in Qingda granules
  • A kind of content determination method of four kinds of alkaloids of Uncaria in Qingda granules

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0065]

[0066] Qingda granules are made from Qingxuan Jiangyatang, and are mainly prepared from the following raw materials by weight: 10-30 parts of Tianma, 10-20 parts of Uncaria, 5-15 parts of lotus seed heart, 5 parts of Scutellaria baicalensis -15 servings. Qingda granules are mainly used for pre-hypertension or grade 1 hypertension. Gastrodia elata is used as the king to calm the liver and yang, supplemented with Uncaria to help clear heat and calm the liver and extinguish wind. It can make the floating yang of the liver hide, and the fire of the heart and liver can be reduced, so as to achieve the hidden yang and calm the liver, suppress the wind, so that the blood pressure can be lowered, and the damage of the heart, brain and kidney can be recovered. Uncaria in the recipe has a variety of effects such as blood pressure, sedation, and antioxidant. The main active ingredients of Uncaria are oxyindole alkaloids such as uricine, isorhynchine, dehydroursinine and isode...

Embodiment

[0104] 1. Experimental materials:

[0105] 1.1 Reagents:

[0106] 1.1.1 Reference substance (for content determination, no need to be treated before use):

[0107] Unchristine, batch number: 112028-201601, purchased from China Institute for Food and Drug Control;

[0108] Isortychnine, batch number: 11927-201403, purchased from China Institute for Food and Drug Control;

[0109] Dehydrourchophylline, item number: ST10570120MG 630-94-4, purity ≥98.0%, purchased from Shanghai Shidander Biotechnology Co., Ltd.;

[0110] Isodehydrourchophylline, item number: ST11080120MG 51014-29-0, purity ≥98.0%, purchased from Shanghai Shidander Biotechnology Co., Ltd.

[0111] 1.1.2 Test article

[0112] Qingda Granules batch numbers: 1905310, 1905001, 1905002, 1905003, provided by Jiangyin Tianjiang Pharmaceutical Co., Ltd.

[0113] 1.1.3 Other reagents:

[0114] Methanol, excellent grade;

[0115] Acetonitrile, chromatographically pure;

[0116] Ammonia, chromatographically pure;

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for determining the content of four alkaloids of Uncaria in Qingda granules. The method includes: preparation of a test solution, preparation of a reference solution, and determination by using an ultra-high performance liquid chromatograph, wherein, The preparation of the test solution includes taking the Qingda granules under the item of the difference in the loading volume, grinding finely, accurately weighing, adding methanol, weighing, sonicating, letting cool, weighing again, and adding methanol to reduce the loss. The weight of the filtrate was filtered, and the subsequent filtrate was accurately drawn through an alkaline alumina column, and the elution solvent was added to elute. The invention solves the problem of fully extracting the related components of Uncaria chinensis from Qingda granules, reduces the damage to the chromatographic column caused by impurities in the detection process, and can also realize the qualitative research of detecting lotus seed hearts.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for determining the content of four alkaloids of Uncaria in Qingda granules. Background technique [0002] Hypertension is a common cardiovascular disease, and its complications are one of the leading causes of human death. In recent years, the prevalence of hypertension has risen rapidly, and its prevention and treatment status is urgent. At present, drug treatment is the most important treatment method for hypertension, among which traditional Chinese medicine treatment has gradually become a hot spot in the early prevention and treatment of hypertension (Xu Hongye, Wang Youqun. Research and application of traditional Chinese medicine in the treatment of hypertension [J]. China Practical Medicine, 2008, 3(25):189-192). Academician Chen Keji, a master of traditional Chinese medicine, believes that the incidence of hypertension has an evolutionary process from Yang...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679Y02A50/30
Inventor 王协和陈盛君陈可冀李松范恺磊杜俊潮
Owner JIANGYIN TIANJIANG PHARMA