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Clostridium perfringens beta toxin mutant protein and its preparation method, application and vaccine

A technology of Clostridium perfringens and β-toxin, which is applied in anti-bacterial immunoglobulin, botany equipment and methods, biochemical equipment and methods, etc. It can solve the problems of poor immunogenicity and protein structure changes, etc. To achieve the effect of toxin safety, good effectiveness and low production cost

Active Publication Date: 2022-06-03
天康制药股份有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In these reports, IPTG was used as an inducer to express in the E. coli system, and a fusion-promoting tag was added at the same time, resulting in changes in the protein structure and poor immunogenicity; at the same time, the β-toxin recombinant protein was expressed in the cell and required ultrasonic disruption and nickel column purification and other complex processes

Method used

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  • Clostridium perfringens beta toxin mutant protein and its preparation method, application and vaccine
  • Clostridium perfringens beta toxin mutant protein and its preparation method, application and vaccine
  • Clostridium perfringens beta toxin mutant protein and its preparation method, application and vaccine

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Embodiment 1

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Embodiment 3

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Abstract

The invention provides a beta toxin mutant protein of Clostridium perfringens and its preparation method, application and vaccine, and relates to the field of biological products. The Clostridium perfringens beta toxin mutant protein includes: the 212th arginine and the 266th tyrosine mutation of the wild-type Clostridium perfringens beta toxin whose amino acid sequence is shown in SEQ ID NO.3 Compared with wild-type Clostridium perfringens beta toxin, the mutant protein of Clostridium perfringens beta toxin has significantly reduced toxicity, while retaining good immunogenicity.

Description

Clostridium perfringens beta toxin mutant protein and its preparation method, application and vaccine technical field The present invention relates to the field of biological products, especially relate to a kind of Clostridium perfringens beta toxin mutant protein and its Preparation methods, applications and vaccines. Background technique Clostridium perfringens (Clostridium Perfringens), also known as Clostridium perfringens or Clostridium perfringens, is a Clostridium, the most common type of gas gangrene pathogens in bed, can break down sugar in muscle and connective tissue, producing a large amount of gas, It leads to severe emphysema of the tissue, which in turn affects the blood supply and causes extensive necrosis of the tissue. Clostridium perfringens is also a sheep epidemic, It is one of the main pathogens of lamb dysentery, necrotizing enteritis of cattle and sheep, and enterotoxemia of cattle and sheep, which has caused huge economic damage to animal h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/33C07K16/12C12N15/31C12N15/74C12N15/75C12N15/77G01N33/569A61K39/08A61P31/04
CPCC07K14/33C07K16/1282C12N15/74C12N15/75C12N15/77G01N33/56911A61K39/08A61P31/04G01N2333/33Y02A50/30
Inventor 贺笋王钢候凤张慧敏周涛刘强德鲍子磊张艳盟姜峰
Owner 天康制药股份有限公司
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