Biological fermentation preparation method of ergosterol peroxide

A technology of ergosterol peroxide and biological fermentation, which is applied in the field of bioengineering and can solve problems such as resource limitation and content instability

Pending Publication Date: 2020-11-17
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, ergosterol peroxide mainly comes from plants or medicinal fungus fruiting bodies, and is limited by resources, and ergosterol supertrophic compounds are unstable due to the influence of geological environment and climate, etc. The purpose of the present invention is to: Provided is a Paecilomyces cicadae mycelium obtained by liquid fermentation of Paecilomyces cicadae, the mycelium of Paecilomyces cicadae is extracted by ethanol, adsorbed and separated by macroporous resin Amberlite XE-243, and purified on a C-18 column to obtain a drug capable of inhibiting renal cell carcinoma. Ergosterol peroxide method

Method used

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  • Biological fermentation preparation method of ergosterol peroxide

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Experimental program
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Embodiment 1

[0034] The preparation of embodiment 1 ergosterol peroxide

[0035] a. with Paecilomyces cicadae (Paecilomyces cicadae, purchased from Beijing Bei Nachuanglian Institute of Biotechnology, No.: BNCC114807) preserved test tube strains as object, Paecilomyces cicadae strains were inoculated in potato glucose medium, 25 Cultivate at 10°C for 7 days, store at 10°C, and prepare Paecilomyces cicadae slant strains;

[0036] b. the Paecilomyces cicadae slant bacterial classification that step a makes is cut into 3 * 3mm size, picks 4 and inoculates in the shake flask that liquid shake flask culture medium is housed, cultivates 7 days at 100rpm, 25 ℃, A liquid shake flask bacterial strain is obtained; wherein, each 1L liquid shake flask culture medium consists of: glucose 10g, corn flour 45g, wheat flour 25g, yeast extract 5g;

[0037] c. the Paecilomyces cicadae liquid shake flask bacterial classification that step b makes is inoculated into the seed tank medium by 5% (shake flask bac...

Embodiment 2

[0039] The preparation of embodiment 2 ergosterol peroxides

[0040] a. Taking Paecilomyces cicadae (a gift from Jiangnan University, No. bio-33088) as an object, inoculate Paecilomyces cicadae in potato glucose medium, cultivate it at 25°C for 3 days, and place it in Preserved at 2°C to obtain Paecilomyces cicadae slant strains;

[0041] b. the Paecilomyces cicadae slant bacterial classification that step a makes is cut into 3 * 3mm size, picks 5 and inoculates in the shake flask that liquid shake flask culture medium is housed, cultivates 6 days at 150rpm, 31 ℃, A liquid shake flask bacterial strain is obtained; wherein, each 1L liquid shake flask culture medium consists of: glucose 31g, corn flour 32g, wheat flour 16g, yeast extract 18g;

[0042] c. the Paecilomyces cicadae liquid shake flask bacterial classification that step b makes is inoculated into the seed tank medium by 20% (shake flask bacterial classification liquid volume / seed tank culture medium liquid volume) i...

Embodiment 3

[0044] The preparation of embodiment 3 ergosterol peroxides

[0045] a. take Paecilomyces cicadae (Paecilomyces cicadae, purchased from Beijing Bei Nachuanglian Institute of Biotechnology, No.: BNCC114807) preserved test tube strains as object, Paecilomyces cicadae strains are inoculated in potato glucose medium, 30 Cultivate for 5 days at ℃, place and preserve at 5 ℃, and prepare Paecilomyces cicadae slant strain;

[0046] b. the Paecilomyces cicadae slant bacterial classification that step a makes is cut into 3 * 3mm size, picks 6 and inoculates in the shake flask that liquid shake flask culture medium is housed, cultivates 3 days at 200rpm, 35 ℃, A liquid shake flask bacterial strain is obtained; wherein, each 1L liquid shake flask culture medium is composed of: glucose 50g, corn flour 15g, wheat flour 5, yeast extract 25g;

[0047] c. the Paecilomyces cicadae liquid shake flask bacterial classification that step b makes is inoculated to the seed tank medium by 13% (shake ...

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Abstract

The invention discloses a biological fermentation preparation method of ergosterol peroxide, and relates to the technical field of bioengineering. Glycerin, yeast powder and peptone are used as main raw materials, and Paecilomyces cicadae mycelium is obtained through liquid shake-flask culture of Paecilomyces cicadae and enlarged culture of a liquid seeding tank. The paecilomyces cicadae myceliumis subjected to ethanol extraction, macroporous resin Amberlite XE-243 separation and C-18 column purification to obtain the ergosterol peroxide. Through experimental study in paecilomyces cicadae liquid fermentation and separation and purification of the ergosterol peroxide from the paecilomyces cicadae liquid fermentation liquid, it proves that the ergosterol peroxide can be used as a medicine for treating kidney tumors, and an ideal medicine is provided for kidney cancer patients.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing ergosterol peroxide through the liquid fermentation technology of Paecilomyces cicadae species that infects cicadas to form cicadas. Background technique [0002] Renal cell carcinoma originates from the epithelial cells of the kidney. It accounts for more than 90 percent of kidney cancers and 3 percent of all fatal, malignancies. Up to 30% of renal cell carcinoma patients experience local recurrence or distant metastasis, and the current five-year survival rate is not ideal. Despite remarkable advances in RCC treatment using first-line targeted tyrosine kinase inhibitors (such as sorafenib or sunitinib), new agents with superior efficacy and safety are still needed in RCC treatment. food or medicine. Natural foods, animals and plants are very valuable resources for drug development, and the utilization of active products is a promising strategy t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P33/00A61K9/28A61K47/38A61K47/36A61K31/58A61P13/12A61P35/00C12R1/79
CPCC12N1/14C12P33/00A61K9/28A61K9/2054A61K9/2059A61K31/58A61P13/12A61P35/00
Inventor 张志才余卓苏琪慧王苗苗史粉慧
Owner JIANGSU UNIV
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