A fructosamine assay kit

A kit and fructosamine technology, applied in the field of fructosamine determination kits, can solve the problems of affecting the stability and accuracy of detection reagents, affecting the accuracy of fructosamine, poor solubility of NBT, etc., to enhance solubility and reagent stability. , Improve the precision and linear range, the effect of excellent linearity and precision

Active Publication Date: 2021-11-19
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, during the detection process, there will be interference from reducing substances such as ascorbic acid, bilirubin, and cholesterol in serum samples, which will affect the accuracy of fructosamine detection results, and it is difficult to effectively solve all interference problems only by relying on a strong alkaline environment; secondly The solubility of NBT is poor, so the substrate will precipitate out during the long-term storage of the reagent, which affects the stability and detection accuracy of the detection reagent.

Method used

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  • A fructosamine assay kit
  • A fructosamine assay kit
  • A fructosamine assay kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The optimization of embodiment 1 R1 reagent

[0023] This embodiment mainly optimizes the R1 reagent, wherein the R1 reagent includes: 250mmol Na 2 CO 3 -NaHCO 3 Buffer solution, 5g / L NaCl, 1g / L sodium azide and Emulgen series surfactants (all are Kao products). Among them, the specific material selection and concentration of Emulegen series surfactants are mainly optimized, as shown in the following table:

[0024] group Emulegen series surfactants concentration 1 Emulgen A90: Emulgen B66 = 1:1 1g / L 2 Emulgen A90 1g / L 3 Emulgen B66 1g / L 4 Emulgen A90: Emulgen 430 = 1:1 1g / L 5 Emulgen B66: Emulgen A60 = 1:1 1g / L 6 Emulgen A500: Emulgen709 = 1:1 1g / L 7 Emulgen A90: Emulgen B66 = 1:1 0.5g / L 8 Emulgen A90: Emulgen B66 = 1:1 2g / L 9 Emulgen A90: Emulgen B66 = 1:1 2.5g / L 10 Surfactant free 0g / L

[0025] The R2 reagent is a conventional reagent, specifically including: pH7.5 100...

Embodiment 2R2

[0031] The optimization of embodiment 2R2 reagent

[0032] R1 reagent is in the present embodiment: 250mmol Na 2 CO 3 -NaHCO 3 Buffer, 5g / L NaCl, 1g / L sodium azide and 1g / L mixture of Emulgen A90 and Emulgen B66 in equal volume ratio. This embodiment mainly optimizes the R2 reagent, as shown in the following table:

[0033] group Citric acid-sodium citrate buffer isopropylamine benzene sulfonate NBT Sodium azide 1 pH 4.5, 15mmol / L 1g / L 2g / L 2g / L 2 pH7.0, 15mmol / L 1g / L 2g / L 2g / L 3 pH3.0, 15mmol / L 1g / L 2g / L 2g / L 4 pH 4.5, 15mmol / L 0.3g / L 2g / L 2g / L 5 pH 4.5, 15mmol / L 2.5g / L 2g / L 2g / L 6 pH 4.0, 15mmol / L 2g / L 2g / L 2g / L 7 pH 5.0, 15mmol / L 0.5g / L 2g / L 2g / L

[0034] Perform performance evaluation on the above 7 groups of kits, including:

[0035] (1) Blank absorbance: the absorbance value of the reagent at a wavelength of 546nm.

[0036] (2) Accuracy: Detect the quality contro...

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Abstract

The invention discloses a fructosamine determination kit, which includes R1 reagent and R2 reagent, wherein the pH of the R1 reagent is 10.0-10.8, including: 200-500mmol / L alkaline buffer solution, 1-10g / L metal salt, 0.5- 2g / L mixture of Emulgen A90 and Emulgen B66 in equal volume ratio, 0.5-2g / L preservative; R2 reagent pH 4.0-5.0, including: 5mmol / L-25mmol / L acidic buffer, 0.5-2g / L isopropylamine phenyl sulfonate; 1~5g / L NBT and 0.5~2g / L preservative. The present invention specially selects the equal volume ratio mixture of Emulgen A90 and Emulgen B66 in the R1 reagent, cooperates with the strong alkaline environment to effectively remove the interfering substances in the serum sample; and in the R2 reagent, the acidic environment and isopropylamine alkyl The benzenesulfonate is used in conjunction with it, and the concentration of the buffer is appropriately reduced to enhance the solubility of NBT and the stability of the reagent, and at the same time improve the precision and linear range of the reagent, thus obtaining a significantly enhanced anti-interference ability and stability , and the linearity and precision of the reagent are also more excellent fructosamine determination kit.

Description

technical field [0001] The invention belongs to the technical field of clinical chemical detection, and in particular relates to a fructosamine determination kit. Background technique [0002] Fructosamine is a substance formed by protein in plasma during non-enzymatic saccharification of glucose. Its concentration is positively correlated with blood sugar level and relatively stable, and the determination of fructosamine is not affected by blood sugar. Since the half-life of plasma protein is 17-20 days, fructosamine can reflect the average blood sugar level of diabetic patients within 1-3 weeks before testing. To a certain extent, it makes up for the deficiency that glycosylated hemoglobin cannot reflect the change of blood sugar concentration in a short period of time, and is a research index for the long-term blood sugar control level of clinical diabetic patients. [0003] There are many methods for the determination of fructosamine, mainly chemical and chromatography....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/66
CPCG01N33/66
Inventor 吴年芬龚婷梁艳张雪娇舒芹
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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