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Method for simultaneously detecting trace residues of 14 drug substances in environmental water body

A technology for detecting environment and drugs, applied in the field of environmental analysis, can solve the problem of high concealment of drug production and trafficking activities, and achieve the effects of traceability of drug sources, simple operation, and accurate quantification

Pending Publication Date: 2020-11-24
GUANGDONG INST OF ANALYSIS CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current popular drug components are diverse, no longer a single methamphetamine component, but a mixture of multiple drug components, and the concealment of drug trafficking activities is high

Method used

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  • Method for simultaneously detecting trace residues of 14 drug substances in environmental water body
  • Method for simultaneously detecting trace residues of 14 drug substances in environmental water body
  • Method for simultaneously detecting trace residues of 14 drug substances in environmental water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] In this embodiment, surface water is selected as the representative of the environmental water body, and a standard addition recovery and sensitivity detection experiment of 14 target drug substances in the surface water sample is provided, including the following steps:

[0060] 1. Sample collection

[0061] Surface water samples were collected from Liuxihe Reservoir in 1L brown glass bottles. Immediately after sample collection, 50 mL of methanol was added to inhibit microbial activity. The sample was transported back to the laboratory as soon as possible in a refrigerated incubator, and the sample extraction was completed within 48 hours.

[0062] 2. Materials and Reagents

[0063] The reagents used in the following are all analytical reagents unless otherwise specified: Water is grade-1 water in accordance with GB / T 6682.

[0064] 2.1 Methamphetamine, Ketamine, Morphine, Codeine, Cocaine, Midazolam, Nitrazepam, Lorazepam, Alprazolam, Clonazepam, Triazolam, Diazepam...

Embodiment 2

[0120] In this example, the extraction effects of 4 kinds of commonly used solid-phase extraction columns on 14 kinds of target drug substances are investigated, as shown in Table 4.

[0121] Table 4: Recovery (%) ± standard deviation (%) of 14 target drug substances in different extraction column conditions

[0122]

[0123]

[0124] Note: Bold indicates that the recovery range exceeds 70% to 120%.

[0125] From Table 4, the recoveries of the 14 target drug substances in the HLB SPE column, ENVI-Carb SPE column, MCX SPE column and MAX SPE column were 49% to 145%, respectively. 50%-259%, 55%-164% and 56%-303%. On the whole, the HLB solid-phase extraction column has a good extraction effect on 14 target drug substances, while the ENVI column has a good extraction effect on the internal standard substance ketamine-D of cocaine. 4 The extraction efficiency of cocaine is low, resulting in a relatively high recovery of cocaine, MAX solid-phase extraction column for cocaine ...

Embodiment 3

[0127] In this example, under different pH conditions, the extraction effect of HLB solid-phase extraction column on 14 target drug substances was investigated, as shown in Table 5.

[0128] Table 5: Recovery rate (%) ± standard deviation (%) of 14 kinds of target drug substances under different pH conditions

[0129]

[0130]

[0131] Note: Bold indicates that the recovery range exceeds 70% to 120%.

[0132] From Table 5, the recoveries of 14 kinds of target drug substances in the HLB solid-phase extraction column under the conditions of pH=3, pH=7 and pH=9 were 43%~1761%, 48%~138% respectively. % and 38% to 151%. Compared with other pH conditions, under the condition of pH=7, the HLB solid-phase extraction column has a better extraction effect on 14 target drug substances, while under the condition of pH=3, the HLB solid-phase extraction column has a better extraction effect. The internal standard substance Ketamine-D of cocaine and chlordiazepoxide 4 The extraction ...

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Abstract

The invention discloses a method for simultaneously detecting trace residues of 14 drug substances in an environmental water body. The method comprises the following steps: S1, adding a filtered watersample into an internal standard solution to obtain a mixed sample solution; loading the mixed sample solution to an activated solid-phase extraction column, drying the solid-phase extraction columnafter loading is completed, eluting 14 target drug substances reserved on the solid-phase extraction column with eluent, combining the eluent, concentrating the eluent, re-dissolving with methanol, and filtering with a filter membrane to obtain a sample extracting solution; and S2, before detection, blowing the sample extracting solution to be nearly dry, re-dissolving by using a mixed solution ofan ammonium acetate-containing formic acid aqueous solution and acetonitrile, and detecting by using an ultra-high performance liquid chromatography / triple quadrupole tandem mass spectrometer. The method provided by the invention has the advantages of strong pertinence, high sensitivity and simple operation, can accurately quantify trace drug substances in the environmental water body, is helpfulfor tracing drug sources, and provides technical support for illegal criminal activities such as drug selling, drug absorption and the like of Ministry of Public Security.

Description

technical field [0001] The invention relates to the technical field of environmental analysis, in particular to a method for simultaneously detecting trace residues of 14 drug substances in environmental water bodies. Background technique [0002] Against the background of the spread of global drug epidemics, the situation of drug control in China is still grim and complicated. On the one hand, criminals mix various drug substances together by changing the form and packaging, and spread and infiltrate members of all levels of society, which is highly camouflaged and confusing, making drug abusers gradually become popular and younger. On the other hand, drug manufacturing activities are increasingly rampant and covert. Criminals manufacture drugs in sparsely populated areas, and the drugs produced are entrained and trafficked through Internet channels, which is highly concealed and harmful, which is not conducive to drug enforcement personnel to trace drug trafficking crimina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14G01N30/04G01N30/34G01N30/86G01N30/88
CPCG01N30/02G01N30/72G01N30/06G01N30/14G01N30/04G01N30/34G01N30/8634G01N30/8679G01N30/88G01N2030/062G01N2030/146G01N2030/045G01N2030/065G01N2030/884
Inventor 姚理马艳芳窦文渊巫培山石燕丽王威姚智锴陈泽炫
Owner GUANGDONG INST OF ANALYSIS CHINA NAT ANALYTICAL CENT GUANGZHOU
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