Nano liposome containing hydrophilic drug core and preparation method of nano liposome
A technology of nano-liposomes and hydrophilic drugs, which is applied in the directions of liposome delivery, medical preparations of inactive ingredients, and drug combinations, etc., can solve the problems of affecting the stability of liposomes and reducing the drug load of drugs, etc. , to achieve the effect of improving stability and drug loading, beneficial to industrial production, and simple preparation process
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Embodiment 1
[0022] The lycopene albumin nano liposome has the following composition in terms of mass percentage: 10% lycopene, 20% bovine serum albumin, 55% soybean lecithin, and 15% cholesterol.
[0023] Concrete preparation steps are as follows:
[0024] (1) Preparation of lycopene-albumin complex: Weigh a specific weight of lycopene and bovine serum albumin and dissolve them in chloroform, with a mass volume ratio of 20%, and place them in a round bottom flask. After mixing and incubating for 30 minutes, chloroform was evaporated on a rotary evaporator under reduced pressure, and purified water was added for hydration to obtain a lycopene-albumin complex with a mass concentration of 10% (w / v).
[0025] (2) Preparation of lycopene-albumin nanoliposomes: Weigh a specific weight of soybean lecithin, dissolve cholesterol in a mixed solvent of ether-n-hexane with a volume ratio of 9:1, and slowly use a syringe during ultrasonication in a water bath. Inject the aqueous solution dissolved wi...
Embodiment 2
[0028] The lycopene albumin nano liposome has the following composition in terms of mass percentage: 10% lycopene, 20% bovine serum albumin, 60% soybean lecithin, and 10% cholesterol.
[0029] Concrete preparation steps are as follows:
[0030] (1) Preparation of lycopene-albumin complex: Weigh a specific weight of lycopene and bovine serum albumin and dissolve them in chloroform, with a mass volume ratio of 30%, and place them in a round bottom flask. After mixing and incubating for 30 minutes, chloroform was evaporated on a rotary evaporator under reduced pressure, and purified water was added for hydration to obtain a lycopene-albumin complex with a mass concentration of 10% (w / v).
[0031] (2) Preparation of lycopene-albumin nanoliposomes: Weigh a specific weight of soybean lecithin, dissolve cholesterol in a mixed solvent of ether-n-hexane with a volume ratio of 9:1, and slowly use a syringe during ultrasonication in a water bath. Inject the aqueous solution dissolved wi...
Embodiment 3
[0034] The lycopene albumin nano liposome has the following composition in terms of mass percentage: 8% lycopene, 15% bovine serum albumin, 62% soybean lecithin, and 15% cholesterol.
[0035] Concrete preparation steps are as follows:
[0036] (1) Preparation of lycopene-albumin complex: Weigh a specific weight of lycopene and bovine serum albumin and dissolve them in chloroform, with a mass volume ratio of 20%, and place them in a round bottom flask. After mixing and incubating for 30 minutes, chloroform was evaporated on a rotary evaporator under reduced pressure, and purified water was added for hydration to obtain a lycopene-albumin complex with a mass concentration of 10% (w / v).
[0037] (2) Preparation of lycopene-albumin nanoliposomes: Weigh a specific weight of soybean lecithin, dissolve cholesterol in a mixed solvent of ether-n-hexane with a volume ratio of 9:1, and slowly use a syringe during ultrasonication in a water bath. Inject the aqueous solution dissolved wit...
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