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Adsorption film for biofilm DGT device, preparation method and application

A biofilm and adsorption film technology, applied in the field of environmental science, can solve the problems of increased sensitivity, high input cost, and reduced cost, and achieve the effects of accurate and effective monitoring, sensitive detection, and improved sensitivity

Active Publication Date: 2020-11-27
南京维申环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem that the sensitivity of the existing biofilm DGT device in the monitoring method of target cell genotoxicity needs to be improved for the monitoring of low-concentration targets, one of the purposes of the present invention is to: , to prepare an adsorption film containing sensing microorganisms and iron oxide, thereby improving the monitoring sensitivity of low-concentration targets
[0009] The second object of the present invention is: in view of the problem that agarose hydrogel needs to be used in the preparation process of the diffusion membrane of the existing biofilm DGT device, and the input cost is relatively high, the diffusion membrane of the DGT device of the present invention can directly use polystyrene Amide hydrogel membrane, the commercially available diffusion membrane can meet the demand and effectively reduce the cost

Method used

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  • Adsorption film for biofilm DGT device, preparation method and application
  • Adsorption film for biofilm DGT device, preparation method and application
  • Adsorption film for biofilm DGT device, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This example is the preparation of the sensory microbial adsorption film, the assembly and sampling of the biofilm DGT device.

[0054] The preparation method of the sensory state microbial adsorption film comprises the following steps:

[0055] 1) First prepare a suspension containing the sensory microorganism ADPWH_recA: the luxCDABE gene is fused into the chromosome of the Acinetobacter baylyi ADP1 host cell to construct;

[0056] 2) The constructed sensory microorganisms were first cultured at a constant temperature (30°C) for 16 hours in a medium (Luria-Bertani, LB) supplemented with 10 μg / mL kanamycin;

[0057] 3) The sensitive microorganisms in each 1 mL of the above medium were recovered by centrifugation (3000 rpm, 10 minutes), and the recovered sensitive microorganisms ADPWH_recA were redissolved in 10 mL of LB medium for use.

[0058] 4) Add agarose powder purchased from Sigma-Aldrich into 100 mL of deionized water, and mix well to obtain an agarose solution...

Embodiment 3

[0067] This embodiment is a comparison of the As concentration in the measured water body of the biofilm DGT device assembled in Example 2 and the conventional DGT device and the relationship with time. The diffusion membrane and the filter membrane of the conventional DGT device are the same as the biofilm DGT device. , the adsorption film adopts the iron oxide adsorption film which has adsorption effect on As. The method for preparing the iron oxide adsorption film is basically the same as the method for preparing the mixed adsorption film in Patent Example 1 with the Chinese patent application number "201510781428.9", except that only ferrous sulfate solution is prepared in the initial solution, and no Introducing aluminum ions and cerium ions, only the iron oxide adsorption film was prepared.

[0068] The above two DGT devices were used to measure the concentration of As in the water and the relationship with time. The specific steps are as follows:

[0069] 1) Solution p...

Embodiment 4

[0079] This example is the relationship between the response of the sensitive microorganism ADPWH_recA to As in the biofilm DGT and the predicted value of the genotoxicity model.

[0080] 1) Solution preparation: prepare As solutions with As concentrations of 1 μg / L, 10 μg / L, 100 μg / L, 1 mg / L, 10 mg / L, and 100 mg / L, the pH value of the solution is 6.0±0.5, and the ionic strength is 0.01 mol / L, the ionic strength is prepared by adding NaNO to deionized water 3 After preparation, the preparation is completed. In an open environment, use a magnetic stirrer to continuously stir the solution for at least 24 hours, so that the carbon dioxide concentration reaches equilibrium, and then start the experiment. During the experimental period, the magnetic stirrer was kept in a stirring state.

[0081] 2) After placing the DGT device of Example 2 in the above-mentioned solutions with different As concentrations for 48 hours, take it out for pretreatment and analysis. The pretreatment pr...

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Abstract

The invention belongs to the field of environmental science, and discloses an adsorption film for a biofilm DGT device, a preparation method and applications thereof. The preparation method comprisesthe following steps: 1) preparing an agarose aqueous solution, and heating and cooling the agarose aqueous solution; 2) uniformly mixing the agarose aqueous solution treated in the step 1) with a suspension containing sensing-state microorganisms to obtain a mixed solution, and preparing a film; and 3) soaking the film in a ferric nitrate solution for a period of time to prepare an agarose hydrogel film containing sensing-state microorganisms and ferric oxide, and rinsing and soaking the film in a buffer solution, carrying out shaking for a period of time, and washing and storing the film. According to the method, the sensing-state microorganisms and the ferric oxide are combined and integrated in the adsorption film, and compared with the mode that in the prior art, the ferric oxide adsorption film serves as the adsorption film, and the sensing-state microorganisms are loaded in the diffusion film, the provided method is more sensitive to toxicity response and is more conducive to accurate monitoring of a low-concentration target As.

Description

technical field [0001] The invention belongs to the field of environmental science, and specifically discloses an adsorption film used for a biofilm DGT device, a preparation method and an application. Background technique [0002] Gradient diffusion film technology (Diffusive gradients in thin films, DGT) is an in-situ, non-destructive passive sampling technology invented by David Williams and Zhang Hao of Lancaster University in the United Kingdom. The gradient diffusion technology invented by British scientists Davison and Zhang Hao Thin-film technology can effectively measure the effective state of heavy metals in nature. Compared with other traditional speciation analysis, it can better reflect the heavy metals absorbed by organisms. It introduces a dynamic concept, which can simulate plants or other organisms. The absorption process of heavy metals is used to study the bioavailability of heavy metals. At present, DGT technology has been widely used in the determination...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30G01N21/64G01N27/62C12Q1/02
CPCB01J20/24B01J20/28033B01J20/28047G01N21/6486G01N27/62G01N33/5005
Inventor 吴双李寒冰昊张·戴维森成皓
Owner 南京维申环保科技有限公司
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