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Photo-thermal immune nanofiber as well as preparation method and application thereof

A nanofiber, photothermal technology, applied in the field of biomedicine, can solve the problems of limited application, unknown side effects, large amount of excipients, etc., and achieves mild and durable immune regulation, enhanced photothermal conversion ability, and good photothermal conversion effect.

Active Publication Date: 2020-12-01
中科南京绿色制造产业创新研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in clinical pre-clinical experiments, the choice of immunomodulatory molecules is often protein macromolecules with complex structures. In the application process of such macromolecules, there are problems such as poor cell penetration, high immunogenicity, unknown side effects, and complex synthesis.
Therefore, the search for small molecules with high activity, clear structure, and clear pathway of action has become an urgent scientific problem to be solved; in addition, for the coupling of immune molecules and other therapeutic modes, it is often used to carry different kinds of active drugs or before and after. The method of separate injection, its low drug concentration, large amount of excipients and cumbersome injection and treatment mode limit further application

Method used

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  • Photo-thermal immune nanofiber as well as preparation method and application thereof
  • Photo-thermal immune nanofiber as well as preparation method and application thereof
  • Photo-thermal immune nanofiber as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] The present embodiment provides a kind of thymopentin-indocyanine green nanofiber, and its preparation method is as follows:

[0055] Prepare thymopentin aqueous solution with a volume mass concentration of 8 mg / mL and indocyanine green aqueous solution with a volume mass concentration of 0.4 mg / mL; add 500 μL thymopentin aqueous solution to 500 μL indocyanine green aqueous solution, and mix well , adjust the pH value of the mixed solution to 7.2 with 0.1mol / L NaOH aqueous solution; age at 4°C in the dark for 24 hours to obtain thymopentin-indocyanine green nanofibers, wherein the molar ratio of thymopentin to indocyanine green It is 23.3:1.

[0056] figure 1 The optical photo of the thymopentin-indocyanine green nanofiber solution obtained for the present embodiment, from figure 1 It can be seen that the obtained nanofibers are uniformly dispersed and have good opalescence.

Embodiment 2

[0058] The present embodiment provides a kind of thymopentin-indocyanine green nanofiber, and its preparation method is as follows:

[0059] Prepare thymopentin aqueous solution with volume mass concentration of 16 mg / mL and indocyanine green aqueous solution with volume mass concentration of 4 mg / mL; after mixing 500 μL thymopentin aqueous solution with 500 μL indocyanine green aqueous solution, use 1mol / L NaOH aqueous solution adjusted the pH value of the mixed solution to 7.2; aged at 25°C for 12 hours in the dark to obtain thymopentin-indocyanine green nanofibers, wherein the molar ratio of thymopentin to indocyanine green was 4.7:1.

[0060] Thymopentin-indocyanine green nanofiber provided by the present embodiment is characterized by transmission electron microscope, the results are as follows figure 2 As shown, it can be seen that the morphology of nanofibers is uniform, and the fiber diameter is <50nm.

Embodiment 3

[0062] Prepare respectively the Thymopentin aqueous solution whose volume mass concentration is 8mg / mL, and the indocyanine green aqueous solution whose volume mass concentration is 0.2mg / mL;

[0063] Sample 1: Mix 500 μL Thymopentin aqueous solution and 500 μL Indocyanine Green aqueous solution, adjust the pH value of the mixed solution to 7.2 with 0.1mol / L NaOH aqueous solution; age at 4°C for 24 hours in the dark to obtain Thymopentin 4mg / mL - Sample 1 of indocyanine green 0.1 mg / mL (the molar ratio of thymopentin to indocyanine green is 46.5:1).

[0064] Sample 2: Mix 20 μL thymopentin aqueous solution and 500 μL indocyanine green aqueous solution, adjust the pH value of the mixed solution to 7.2 with 0.1mol / L NaOH aqueous solution, dilute to 1mL with ultrapure water and disperse evenly; at 25°C Aging in the dark for 12 hours, sample 2 of Thymopentin 0.16mg / mL-Indocyanine Green 0.1mg / mL was obtained (the molar ratio of Thymopentin to Indocyanine Green was 1.9:1);

[0065]...

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Abstract

The invention provides a photo-thermal immune nanofiber as well as a preparation method and application thereof. The photo-thermal immune nanofiber comprises immune active peptide and a photo-thermalreagent, and the molar ratio of the immune active peptide to the photo-thermal reagent is (4-50):1. The photo-thermal immune nanofiber is formed by mixing an immune active peptide solution and the photo-thermal reagent solution and regulating and assembling the photo-thermal reagent by utilizing immune active molecules under the interaction of hydrogen bonds, static electricity and the like. The photo-thermal immune nanofiber provided by the invention can give play to the advantages of photo-thermal treatment and immune regulation at the same time, has the advantages of good photo-thermal conversion effect, mild and lasting immune regulation effect and adjustable absorption wavelength, and can be used for preparing photo-thermal immune combined treatment medicines for tumors.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a photothermal immunity nanofiber, its preparation method and application. Background technique [0002] In recent years, the rapid development of tumor immunotherapy has provided a powerful weapon for the treatment of malignant tumors. However, at present, its mild regulatory effect can only exert 20% of the therapeutic effect in clinical applications. Therefore, immunomodulation combined with other therapeutic modes has become the main strategy to improve the clinical therapeutic effect. Among them, photothermal therapy is a treatment mode with simple operation and biological safety. This treatment method uses photothermal reagents to quickly convert the absorbed light energy into heat, resulting in local high temperature of tumor lesions, and achieving necrosis or apoptosis of tumor cells. . Based on this, the combination of photothermal immunity shows unique advantage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/70A61P35/00A61P35/04A61K38/08A61K38/05A61K38/06
CPCA61K41/0052A61K38/08A61K38/05A61K38/06A61K9/70A61P35/00A61P35/04A61K2300/00
Inventor 闫学海李淑坤邢蕊蕊
Owner 中科南京绿色制造产业创新研究院