Reagent composition for skin care
A technology of reagents and thickeners, applied in the field of skin care products, can solve the problems of large molecular weight of polypeptides, poor practicability, and affecting skin care effects
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[0063] The preparation method of umbilical cord mesenchymal stem cell exosome extract comprises the following steps:
[0064] Step 1. Culture of umbilical cord mesenchymal stem cells
[0065] Well-grown umbilical cord mesenchymal stem cells were taken and cultured in α-MEM medium until they grew to a confluence of more than 80%. The culture medium was collected, washed three times with PBS, digested and subcultured with 0.25% trypsin, and continued to be subcultured. The culture supernatant was collected once and replaced with fresh medium, and the culture supernatant was collected during continuous culture.
[0066] Step 2. Extraction of exosomes
[0067] a. Take the cell culture supernatant and centrifuge at 300-400g for 10-20min, collect the supernatant, and discard the precipitate;
[0068] b. Take the supernatant collected in step a and centrifuge at 2000-3000g for 20-30min, continue to collect the supernatant, and discard the precipitate;
[0069] c. Filter the supern...
Embodiment 1
[0116] This embodiment provides a reagent combination for skin care, the reagent combination comprises a first reagent, a second reagent and a third reagent, wherein, in parts by weight,
[0117] The first reagent comprises: 1 part of glycerin, 1 part of butanediol, 0.1 part of sodium hyaluronate and 80.8 parts of water;
[0118] The second reagent comprises: 1 part of tranexamic acid, 1 part of ascorbic acid, 0.1 part of dipotassium glycyrrhizinate and 14.9 parts of water;
[0119] The third reagent comprises: 0.1 part of umbilical cord mesenchymal stem cell exosome extract.
[0120] The preparation method of the reagent combination that this embodiment is used for skin care is as follows:
[0121] Step 1. Add 1 part of glycerin, 1 part of butanediol and 0.1 part of sodium hyaluronate into 80.8 parts of water, heat to 80°C, stir well to dissolve, then cool to below 40°C to obtain the first reagent;
[0122] Step 2. Add 1 part of tranexamic acid, 1 part of ascorbic acid and ...
Embodiment 2
[0126] This embodiment provides a reagent combination for skin care, the reagent combination comprises a first reagent, a second reagent and a third reagent, wherein, in parts by weight,
[0127] The first reagent comprises: 6 parts of glycerin, 6 parts of butanediol, 1.5 parts of sodium hyaluronate and 68.5 parts of water;
[0128] The second reagent comprises: 4 parts of tranexamic acid, 4 parts of ascorbic acid, 1 part of dipotassium glycyrrhizinate and 8 parts of water;
[0129] The third reagent includes: 1 part of umbilical cord mesenchymal stem cell exosome extract.
[0130] The preparation method of the reagent combination that this embodiment is used for skin care is as follows:
[0131] Step 1. Add 6 parts of glycerin, 6 parts of butanediol and 1.5 parts of sodium hyaluronate into 68.5 parts of water, heat to 80°C, fully stir and dissolve, then cool to below 40°C to obtain the first reagent;
[0132] Step 2. Add 4 parts of tranexamic acid, 4 parts of ascorbic acid ...
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