Method for cultivating pentaploid seedless momordica grosvenori
A cultivation method and technique of Monk fruit, applied in the field of plant cultivation, can solve the problems of high production cost, lack, low mogroside content, etc., and achieve the effect of great economic value
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Embodiment 1
[0042] Choose a Luo Han Guo variety with larger fruit (70-80g per fruit) and about 0.46% sweet glycoside V content, peel off 120 seeds and clean them in tap water, put them in a container after shelling, and rinse them with sterile water for 3 times , until the liquid is clear, add 75% alcohol to disinfect for 2 minutes, pour out the alcohol, add 40% sodium hypochlorite and place it on a shaker, sterilize at 150rpm for 20 minutes, and finally rinse with sterile water for 5 times, pour out the water and set aside .
[0043] Put 8-10 layers of neutral sterile filter paper in a sterile petri dish, add sterile water until the sterile filter paper is completely submerged and no water flows. Put the sterilized Luo Han Guo seeds on the filter paper and cover it with a lid Seal and culture in the dark at 36-38°C for 2 days, then at 25-26°C for 5 days.
[0044] Select 100 seeds whose embryos have begun to germinate during pre-cultivation, put them into a sterile petri dish with steril...
Embodiment 2
[0066] Choose the Luo Han Guo variety with larger fruit and about 0.45% sweet glycoside V content, peel off 120 seeds and wash them in tap water, put them in a container after shelling, and rinse them with sterile water for 3 times until the liquid is clear. Add 75% alcohol to disinfect for 2 minutes, pour out the alcohol, add 40% sodium hypochlorite and place it on a shaker, disinfect at 150rpm for 20 minutes, and finally rinse with sterile water 5 times, pour out the water and set aside.
[0067] Put 8-10 layers of neutral sterile filter paper in a sterile petri dish, add sterile water until the sterile filter paper is completely submerged and no water flows. Put the sterilized Luo Han Guo seeds on the filter paper and cover it with a lid Sealed, cultured in the dark at 38-40°C for 2 days, and then cultured at 25-26°C for 5 days.
[0068] Select 100 seeds whose embryos have begun to germinate during pre-cultivation, put them into a sterile petri dish with sterile filter pape...
Embodiment 3
[0088] Choose the Luo Han Guo variety with larger fruit and about 0.45% sweet glycoside V content, peel off 120 seeds and wash them in tap water, put them in a container after shelling, and rinse them with sterile water for 3 times until the liquid is clear. Add 75% alcohol to disinfect for 2 minutes, pour out the alcohol, add 40% sodium hypochlorite and place it on a shaker, disinfect at 150rpm for 20 minutes, and finally rinse with sterile water 5 times, pour out the water and set aside.
[0089] Put 8-10 layers of neutral sterile filter paper in a sterile petri dish, add sterile water until the sterile filter paper is completely submerged and no water flows. Put the sterilized Luo Han Guo seeds on the filter paper and cover it with a lid Sealed, cultured in the dark at 35-37°C for 2 days, and then cultured at 25-26°C for 5 days.
[0090] Select 100 seeds whose embryos have begun to germinate during pre-cultivation, put them into a sterile petri dish with sterile filter pape...
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