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Chinese yam polysaccharide extraction method

An extraction method and technology of Huaishan polysaccharide, applied in the field of extraction of Huaishan polysaccharide, can solve the problems of large solvent consumption, low polysaccharide yield, easy gelatinization, etc., to improve yield and purity, improve enzymatic hydrolysis efficiency, and reduce gelatinization. Effect

Active Publication Date: 2020-12-11
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods commonly used in yam polysaccharides include hot water extraction, ultrasonic-assisted extraction, microwave-assisted extraction and enzyme-assisted extraction, but these extraction processes generally have two major problems: one is the lack of effective measures to remove yam starch
The mass fraction of starch in yam is as high as 70% (DW), of which amylopectin is 2-3 times that of amylose. Because the extraction temperature of polysaccharides is generally high, amylopectin is easy to gelatinize after boiling. If the starch is not treated, It will cause the extracted yam polysaccharide to contain starch; second, the traditional extraction method takes a long time, consumes a lot of solvent, and the yield of polysaccharide is low, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The configuration of urea-choline chloride deep eutectic solvent: mix urea, choline chloride and water with a mass ratio of 30:14:11 to obtain a urea-choline chloride deep eutectic solvent;

[0065] Preparation of yam freeze-dried powder: select vacuum freeze-dried yam, crush it and pass through an 80-mesh sieve to get yam freeze-dried powder;

[0066] Configuration of sevag reagent: mix chloroform and n-butanol at a volume ratio of 5:1 to obtain sevag reagent;

[0067] (1) Mix 400mL of urea-choline chloride deep eutectic solvent with 10g of Chinese yam freeze-dried powder, conduct ultrasonic-assisted extraction at 100W for 30min, and extract in a water bath at 100°C for 40min. After the extraction is completed, cool to room temperature and collect the extract spare;

[0068] (2) Add 9.2mL of α-amylase aqueous solution (140U / mL) to the above extract, and hydrolyze it for 50min at 50°C to obtain a primary enzymolysis solution; Lulanase aqueous solution (230U / mL), hydro...

Embodiment 2

[0073] The configuration of the urea-choline chloride deep eutectic solvent: urea, choline chloride and water are mixed with a mass ratio of 30:14:11 to obtain the urea-choline chloride deep eutectic solvent;

[0074] Preparation of yam freeze-dried powder: select vacuum freeze-dried yam, crush it and pass through a 60-mesh sieve to get yam freeze-dried powder;

[0075] Configuration of sevag reagent: mix chloroform and n-butanol at a volume ratio of 4:1 to obtain sevag reagent;

[0076] (1) Mix 500mL of urea-choline chloride deep eutectic solvent with 10g of Chinese yam freeze-dried powder, conduct ultrasonic-assisted extraction at 200W for 20min, and extract in a water bath at 80°C for 60min. After the extraction is completed, cool to room temperature and collect the extract spare;

[0077] (2) Add 10mL of α-amylase aqueous solution (150U / mL) to the above extract, and hydrolyze it for 60min at 51°C to obtain a primary enzymolysis solution; Lanzyme aqueous solution (250U / mL...

Embodiment 3

[0082] The configuration of the urea-choline chloride deep eutectic solvent: urea, choline chloride and water are mixed with a mass ratio of 30:14:11 to obtain the urea-choline chloride deep eutectic solvent;

[0083] Preparation of yam freeze-dried powder: select vacuum freeze-dried yam, crush it and pass through an 80-mesh sieve to get yam freeze-dried powder;

[0084]Configuration of sevag reagent: mix chloroform and n-butanol at a volume ratio of 6:1 to obtain sevag reagent;

[0085] (1) Mix 300mL of urea-choline chloride deep eutectic solvent with 10g of yam freeze-dried powder, extract with ultrasonic assistance for 30min at 150W, and extract in a water bath at 90°C for 40min. After the extraction is completed, cool to room temperature and collect the extract spare;

[0086] (2) Add 7.5mL of α-amylase aqueous solution (120U / mL) to the above extract, and hydrolyze it for 50min at 52°C to obtain a first-level enzymolysis solution; Lanzyme aqueous solution (200U / mL), hydr...

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Abstract

The invention relates to the field of plant polysaccharide extraction, and discloses a Chinese yam polysaccharide extraction method. The method comprises the steps of (1) mixing Chinese yam and a urea-choline chloride eutectic solvent, carrying out heating extraction, cooling, and separating to obtain an extracting solution; (2) mixing the extracting solution obtained in the step (1) with an enzyme, carrying out enzymolysis, and carrying out solid-liquid separation on an enzymolysis product to obtain a filtrate; and (3) carrying out alcohol precipitation on the filtrate obtained in the step (2), and washing to obtain a crude Chinese yam polysaccharide precipitate. According to the extraction method, starch contained in the Chinese yams can be effectively removed, the extraction efficiencyof the Chinese yam polysaccharide is improved, and the extraction method is environmentally friendly, efficient and free of pollution.

Description

technical field [0001] The invention relates to the field of extracting plant polysaccharides, in particular to a method for extracting yam polysaccharides. Background technique [0002] Huaishan, also known as yam, yam, etc., is a high-quality crop of medicine, vegetable, and grain in my country. It is rich in various nutrients, such as starch, polysaccharides, allantoin, choline, saponins, minerals, etc. Among them, polysaccharides It is considered as its main active substance, and its content reaches 0.5%-10.0% (DW). Most active polysaccharides have good pharmacological activity and unique physical and chemical properties, such as: ginseng polysaccharides that have been used in clinical treatment of tumors, cordyceps polysaccharides used as immune enhancers, and pectin used as food additives, etc. Yam polysaccharides have also been used It has been proven to have functions such as anti-oxidation, hypoglycemia, anti-tumor, and immune regulation. Under certain conditions, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/10
Inventor 王锋张锦钰苏小军李清明秦丹郭红英刘志伟
Owner HUNAN AGRICULTURAL UNIV
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